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Protein

ENHANCER OF AG-4 protein 2

Gene

HUA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that functions as repressor of flowering by enhancing the expression of several genes that delay flowering including FLC, FLM/MAF1, MAF2 and SVP (PubMed:15659097). Also acts in the floral homeotic AGAMOUS (AG) pathway, specifically by processing the AGAMOUS pre-mRNA (PubMed:10198637, PubMed:12530963). Functions in association with HUA1 and HEN4 in AG pre-mRNA processing (PubMed:12530963). Involved in all three aspects of the AG functions, the specification of stamen and carpel identities, the control of floral determinacy, and the spatial restriction of AP1 expression (PubMed:10198637, PubMed:12530963, PubMed:15659097). Acts as transcription regulator that controls anthocyanin accumulation (PubMed:25425527).4 Publications

GO - Molecular functioni

  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • anthocyanin accumulation in tissues in response to UV light Source: UniProtKB
  • cell differentiation Source: UniProtKB-KW
  • maintenance of floral organ identity Source: TAIR
  • mRNA processing Source: UniProtKB
  • negative regulation of flower development Source: TAIR
  • regulation of timing of transition from vegetative to reproductive phase Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Flowering, mRNA processing, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
ENHANCER OF AG-4 protein 2
Alternative name(s):
Protein AERIAL ROSETTE 1
Gene namesi
Name:HUA21 Publication
Synonyms:ART1
Ordered Locus Names:At5g23150Imported
ORF Names:MKD15, MYJ24.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G23150.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Hua2 mutations enhance the phenotype of mild ag-4 and elf4 alleles and also induce early flowering by reducing the expression of several MADS genes that act as floral repressors like FLC, FLM/MAF1, MAF2 and SVP.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13921392ENHANCER OF AG-4 protein 2PRO_0000418856Add
BLAST

Proteomic databases

PaxDbiQ9XER9.
PRIDEiQ9XER9.

PTM databases

iPTMnetiQ9XER9.

Expressioni

Tissue specificityi

Expressed in the inflorescence meristem, floral primordia, inflorescence stem, and floral pedicels. Also detected in the shoot apical meristem, stems, leaves, embryos, and roots.1 Publication

Gene expression databases

GenevisibleiQ9XER9. AT.

Interactioni

Protein-protein interaction databases

BioGridi17654. 6 interactions.
STRINGi3702.AT5G23150.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XER9.
SMRiQ9XER9. Positions 21-71, 774-912.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 7758PWWPPROSITE-ProRule annotationAdd
BLAST
Domaini771 – 912142CIDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1056 – 1156101Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 CID domain.PROSITE-ProRule annotation
Contains 1 PWWP domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410ZJEY. LUCA.
HOGENOMiHOG000090612.
InParanoidiQ9XER9.
OMAiPARPDIH.
OrthoDBiEOG093600BT.
PhylomeDBiQ9XER9.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR006569. CID_dom.
IPR008942. ENTH_VHS.
IPR000313. PWWP_dom.
IPR006903. RNA_pol_II-bd.
[Graphical view]
PfamiPF04818. CTD_bind. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
SM00582. RPR. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS51391. CID. 1 hit.
PS50812. PWWP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XER9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPGRKRGAS KAKAKGQLVL GDLVLAKVKG FPAWPAKISR PEDWDRAPDP
60 70 80 90 100
KKYFVQFFGT EEIAFVAPPD IQAFTSEAKS KLLARCQGKT VKYFAQAVEQ
110 120 130 140 150
ICTAFEGLQN HKSNALGDED SLDATEPGLT KAEIVDGTDH IVIESERTDN
160 170 180 190 200
FNFRVDPCFP KLDENNGEER KAEIRKLDSS SFLESKVKTT SPVSESLEHS
210 220 230 240 250
SFDPKIKKED FDKGTDGSAC NEHFGNGQKK LANGKRIKKE AGGSDRKGED
260 270 280 290 300
TVHRDKSNNS HVPGGRTASG NSDSKKSKGL LTEKTSSKVS ADKHENSPGI
310 320 330 340 350
KVGVSGKKRR LESEQGKLAP RVDESSRAAK KPRCESADNK VKCEIDDGSD
360 370 380 390 400
STGTVSDIKR EIVLGLGARG GNFQYDKEAV AYTKRQRQTM EHATSPSFSG
410 420 430 440 450
SRDKSGKGHL EQKDRSSPVR NVKAPAAQSL KKRRAVCIYD EDDDEDPKTP
460 470 480 490 500
LHGKPAIVPQ AASVLTDGPK RANVCHSTST KAKISAGSTE STEVRKFPLR
510 520 530 540 550
KHCEDASRVL PSNAENSTNS LPVVKPINEL PPKDVKQILQ SPKMSPQLVL
560 570 580 590 600
TNKHVAGQHK VVKSSVKVSG VVMAKKPQSD SCKEAVAGSD KISSSQSQPA
610 620 630 640 650
NQRHKSASVG ERLTVVSKAA SRLNDSGSRD MSEDLSAAML DLNREKGSAT
660 670 680 690 700
FTSAKTPDSA ASMKDLIAAA QAKRKLAHTQ NSIFGNLNPS FLSISDTQGR
710 720 730 740 750
SHSPFMVQNA SASAAISMPL VVQGHHQQGS SPSNHGHQSL SRNQIETDDN
760 770 780 790 800
EERRLSSGHK SVGGSLSCST EAAISRDAFE GMLETLSRTR ESIGRATRLA
810 820 830 840 850
IDCAKYGLAS EVVELLIRKL ESESHFHRKV DLFFLVDSIT QHSHSQKGIA
860 870 880 890 900
GASYVPTVQA ALPRLLGAAA PPGTGASDNR RKCLKVLKLW LERKVFPESL
910 920 930 940 950
LRRYIDDIRA SGDDATGGFS LRRPSRSERA VDDPIREMEG MLVDEYGSNA
960 970 980 990 1000
TFQLPGFFSS HNFEDDEEDD DLPTSQKEKS TSAGERVSAL DDLEIHDTSS
1010 1020 1030 1040 1050
DKCHRVLEDV DHELEMEDVS GQRKDVAPSS FCENKTKEQS LDVMEPVAEK
1060 1070 1080 1090 1100
STEFNPLPED SPPLPQESPP PLPPLPPSPP PPSPPLPPSS LPPPPPAALF
1110 1120 1130 1140 1150
PPLPPPPSQP PPPPLSPPPS PPPPPPPPSQ SLTTQLSIAS HHQIPFQPGF
1160 1170 1180 1190 1200
PPPTYPLSHQ TYPGSMQQDR SSIFTGDQIV QGPGNSSRGG LVEGAGKPEY
1210 1220 1230 1240 1250
FVQQSSSFSP AGVCSSREPS SFTSSRQLEF GNSDVLFNPE ASSQNHRFQP
1260 1270 1280 1290 1300
STPLSQRPMV RLPSAPSSHF SYPSHIQSQS QHSYTHPYPF PPQRDDARRY
1310 1320 1330 1340 1350
RNEEPWRIPS SGHSAENQNG AWIHGRNSHP GLPRVTDSFF RPPPERPPSG
1360 1370 1380 1390
TMNYQPSAAS NLQAVPAIPG HTAPQMLPSR PDIPTVNCWR PA
Length:1,392
Mass (Da):151,078
Last modified:November 1, 1999 - v1
Checksum:iD2AC1B0D2AA70F0F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti525 – 5251K → E in strain: cv. Sy-0; causes late flowering phenotype. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116556 mRNA. Translation: AAD31171.1.
AB007648, AB006708 Genomic DNA. Translation: BAB11170.1.
CP002688 Genomic DNA. Translation: AED93127.1.
AB493755 mRNA. Translation: BAH30593.1.
PIRiT51947.
RefSeqiNP_197706.1. NM_122221.2.
UniGeneiAt.11269.

Genome annotation databases

EnsemblPlantsiAT5G23150.1; AT5G23150.1; AT5G23150.
GeneIDi832379.
GrameneiAT5G23150.1; AT5G23150.1; AT5G23150.
KEGGiath:AT5G23150.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116556 mRNA. Translation: AAD31171.1.
AB007648, AB006708 Genomic DNA. Translation: BAB11170.1.
CP002688 Genomic DNA. Translation: AED93127.1.
AB493755 mRNA. Translation: BAH30593.1.
PIRiT51947.
RefSeqiNP_197706.1. NM_122221.2.
UniGeneiAt.11269.

3D structure databases

ProteinModelPortaliQ9XER9.
SMRiQ9XER9. Positions 21-71, 774-912.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17654. 6 interactions.
STRINGi3702.AT5G23150.1.

PTM databases

iPTMnetiQ9XER9.

Proteomic databases

PaxDbiQ9XER9.
PRIDEiQ9XER9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G23150.1; AT5G23150.1; AT5G23150.
GeneIDi832379.
GrameneiAT5G23150.1; AT5G23150.1; AT5G23150.
KEGGiath:AT5G23150.

Organism-specific databases

TAIRiAT5G23150.

Phylogenomic databases

eggNOGiKOG1904. Eukaryota.
ENOG410ZJEY. LUCA.
HOGENOMiHOG000090612.
InParanoidiQ9XER9.
OMAiPARPDIH.
OrthoDBiEOG093600BT.
PhylomeDBiQ9XER9.

Miscellaneous databases

PROiQ9XER9.

Gene expression databases

GenevisibleiQ9XER9. AT.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR006569. CID_dom.
IPR008942. ENTH_VHS.
IPR000313. PWWP_dom.
IPR006903. RNA_pol_II-bd.
[Graphical view]
PfamiPF04818. CTD_bind. 1 hit.
PF00855. PWWP. 1 hit.
[Graphical view]
SMARTiSM00293. PWWP. 1 hit.
SM00582. RPR. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS51391. CID. 1 hit.
PS50812. PWWP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUA2_ARATH
AccessioniPrimary (citable) accession number: Q9XER9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: November 1, 1999
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In cv. Sy-0, a naturally occurring allele K525E, termed ART1, together with active FRI and FLC alleles causes delayed flowering of primary and axillary meristems, resulting in the distinctive Sy-0 morphology (PubMed:17764945).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.