Reviewed, UniProtKB/Swiss-Prot Q9XEL8 (HMDH2_CAPAN)

Names and origin

Protein names

Recommended name:
    3-hydroxy-3-methylglutaryl-coenzyme A reductase 2
      Short name=HMG-CoA reductase 2
    EC=1.1.1.34

Gene names

Name:

HMGR2

Organism

Capsicum annuum (Bell pepper)

Taxonomic identifier

4072 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
Catalytic activity	(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
Pathway	Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the HMG-CoA reductase family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	coenzyme A metabolic process Inferred from electronic annotation. Source: InterPro isoprenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro hydroxymethylglutaryl-CoA reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	3-hydroxy-3-methylglutaryl-coenzyme A reductase 2		PRO_0000114436
Regions
	Transmembrane	47 – 67	21	Potential
	Transmembrane	91 – 111	21	Potential
	Region	112 – 188	77	Linker By similarity
	Region	189 – 604	416	Catalytic By similarity
Sites
	Active site	283	1	Charge relay system By similarity
	Active site	415	1	Charge relay system By similarity
	Active site	491	1	Charge relay system By similarity
	Active site	589	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	347	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
	Glycosylation	593	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XEL8-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: CCCE77884BC5A362	FASTA	604	64,881
	10 20 30 40 50 60 MDVRRRSEEA VYSSKVFAAD EKPLKPHKQQ QEEDNTLLID ASDALPLPLY FTNGLFFTMF 70 80 90 100 110 120 FSVMYFLLSR WREKIRNSTP LHVVTLSELG AIVSLIASVI YLLGFFGIGF VQTFVARGNN 130 140 150 160 170 180 DSWDEEDEND EQFILEEDSR RGPCAAATTL GCAVPTPPAK HIAPIVPQQP AVSIAEKPAP 190 200 210 220 230 240 LVTPAASEED EEIIKSVVQG KIPSYSLESK LGDCKRAASI RKEVLQRITG KSLEGLPLDG 250 260 270 280 290 300 FNYESILGQC CEMTIGYVQI PVGIAGPLLL NGREYSVPMA TTEGCLVAST NRGCKAIYAS 310 320 330 340 350 360 GGATSILLRD GMTRAPCVRF GTAKRAAELK FFVEDPINFE TLANVFNQSS RFARLQRIQC 370 380 390 400 410 420 AIAGKNLHMR FVCSTGDAMG MNMVSKGVQN VLDYLQNEYA DMDVIGISAN FCSDKKPAAV 430 440 450 460 470 480 NWIEGRGKSV VCEAIITEEV VKKVLKTEVA ALVELNMLKN LTGSALAGAL GGFNAHASNI 490 500 510 520 530 540 VSAVYIATGQ DPAQNIESSH CITMMEAVND GKDLHISVTM PSIEVGTVGG GTQLASQSAC 550 560 570 580 590 600 LNLLGVKGAN REAPGSNARL LATIVAGSVL AGELSLMSAI SAGQLVNSHM KYNRSTKDVT KASS

References

[1]	"Pathogen-inducible HMGR2 gene from hot pepper." Ha S.H., Kim J.B., Lee S.W., Hwang Y.S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF110383 mRNA. Translation: AAD28179.1.
3D structure databases
SMR	Q9XEL8. Positions 186-586.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.34. 1261.
Family and domain databases
InterPro	IPR002202. HMG_CoA_Rdtase_cat. IPR004554. HMG_CoA_Rdtase_I_cat. IPR009023. HMG_CoA_Rdtase_NAD(P)-bd. IPR009029. HMG_CoA_Rdtase_sub-bd. [Graphical view]
Gene3D	G3DSA:3.90.770.10. HMG-CoA_red. 1 hit.
PANTHER	PTHR10572. HMG-CoA_red. 1 hit.
Pfam	PF00368. HMG-CoA_red. 1 hit. [Graphical view]
PRINTS	PR00071. HMGCOARDTASE.
TIGRFAMs	TIGR00533. HMG_CoA_R_NADP. 1 hit.
PROSITE	PS00066. HMG_COA_REDUCTASE_1. 1 hit. PS00318. HMG_COA_REDUCTASE_2. 1 hit. PS01192. HMG_COA_REDUCTASE_3. 1 hit. PS50065. HMG_COA_REDUCTASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HMDH2_CAPAN

Accession

Primary (citable) accession number: Q9XEL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)