Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

ALG11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.1 Publication

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

Pathwayi

GO - Molecular functioni

  1. GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mannosylation Source: GOC
  2. oligosaccharide-lipid intermediate biosynthetic process Source: UniProtKB
  3. plant-type cell wall biogenesis Source: TAIR
  4. protein N-linked glycosylation Source: TAIR
  5. response to abscisic acid Source: TAIR
  6. response to osmotic stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_250789. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Protein LEAF WILTING 3
Gene namesi
Name:ALG11
Synonyms:LEW3
Ordered Locus Names:At2g40190
ORF Names:T07M07.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G40190.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence AnalysisAdd
BLAST
Transmembranei204 – 22623HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Lethality.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi148 – 1481G → E in lew3; deficiency in N-glycosylation leading to dwarf phenotype, reduced fertility and impaired cellulose synthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferasePRO_0000412585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9XEE9.

Expressioni

Tissue specificityi

Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.1 Publication

Gene expression databases

GenevestigatoriQ9XEE9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40190.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9XEE9.
SMRiQ9XEE9. Positions 272-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 494Poly-Gly

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiQ9XEE9.
KOiK03844.
OMAiGTVILAH.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9XEE9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIYFILYTL LTIIFAVSLS LFLSVINARK SRKRAVGFFH PYTNDGGGGE
60 70 80 90 100
RVLWCAVKAI QEENPDLDCV IFTGDHDSSS DSLARRAVDR FGVHLQSPPK
110 120 130 140 150
VIHLNKRKWI EESTYPHFTM IGQSLGSVYL AWEALRMFTP LYFLDTSGYA
160 170 180 190 200
FTYPLARIFG CKVVCYTHYP TISLDMISRV RQRNSMYNND ASIAKSNWLS
210 220 230 240 250
TCKLVYYRAF SWMYGMVGSC THLAMVNSSW TKSHIEVLWR IPERITRVYP
260 270 280 290 300
PCDTSGLQAF PLERSSDPPK IISVAQFRPE KAHMLQLEAF SLALEKLDAD
310 320 330 340 350
VPRPKLQFVG SCRNNSDEER LQKLKDRAVE LKVDGDVQFY KNAMYRELVE
360 370 380 390 400
LLGNAVAGLH GMIDEHFGIS VVEYMAAGAI PIAHNSAGPK MDIVLEEDGQ
410 420 430 440 450
KTGFLAETVE EYAEAILEIV KMNETERLKM AESARKRAAR FSEQRFCEDF
460
KTAIRPIFTG PLK
Length:463
Mass (Da):52,573
Last modified:September 21, 2011 - v2
Checksum:i7092C34149CD4AE1
GO
Isoform 2 (identifier: Q9XEE9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-212: Missing.

Show »
Length:251
Mass (Da):28,261
Checksum:iCFB26F285390374C
GO

Sequence cautioni

The sequence AAD25934.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 212212Missing in isoform 2. 1 PublicationVSP_041714Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1.
AK227731 mRNA. Translation: BAE99716.1.
BT009712 mRNA. Translation: AAP88346.1.
PIRiD84826.
RefSeqiNP_181548.2. NM_129577.4. [Q9XEE9-1]
UniGeneiAt.21597.

Genome annotation databases

EnsemblPlantsiAT2G40190.1; AT2G40190.1; AT2G40190. [Q9XEE9-1]
GeneIDi818610.
KEGGiath:AT2G40190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1.
AK227731 mRNA. Translation: BAE99716.1.
BT009712 mRNA. Translation: AAP88346.1.
PIRiD84826.
RefSeqiNP_181548.2. NM_129577.4. [Q9XEE9-1]
UniGeneiAt.21597.

3D structure databases

ProteinModelPortaliQ9XEE9.
SMRiQ9XEE9. Positions 272-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G40190.1-P.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEiQ9XEE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40190.1; AT2G40190.1; AT2G40190. [Q9XEE9-1]
GeneIDi818610.
KEGGiath:AT2G40190.

Organism-specific databases

TAIRiAT2G40190.

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiQ9XEE9.
KOiK03844.
OMAiGTVILAH.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_250789. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ9XEE9.

Gene expression databases

GenevestigatoriQ9XEE9.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07."
    Wang M.L., Belmonte S., Kim U., Dolan M., Morris J.W., Goodman H.M.
    Genome Res. 9:325-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Morosawa T., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K.
    , Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-463.
  6. "LEW3, encoding a putative alpha-1,2-mannosyltransferase (ALG11) in N-linked glycoprotein, plays vital roles in cell-wall biosynthesis and the abiotic stress response in Arabidopsis thaliana."
    Zhang M., Henquet M., Chen Z., Zhang H., Zhang Y., Ren X., van der Krol S., Gonneau M., Bosch D., Gong Z.
    Plant J. 60:983-999(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-148, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiALG11_ARATH
AccessioniPrimary (citable) accession number: Q9XEE9
Secondary accession number(s): F4IH01, Q0WT32, Q7DNH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: January 7, 2015
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG11 activity, no N-glycans are produced and transferred to proteins.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.