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Q9XEE9

- ALG11_ARATH

UniProt

Q9XEE9 - ALG11_ARATH

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Protein
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Gene
ALG11, LEW3, At2g40190, T07M07.7
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.1 Publication

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

Pathwayi

GO - Molecular functioni

  1. GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  1. mannosylation Source: GOC
  2. oligosaccharide-lipid intermediate biosynthetic process Source: UniProtKB
  3. plant-type cell wall biogenesis Source: TAIR
  4. protein N-linked glycosylation Source: TAIR
  5. response to abscisic acid Source: TAIR
  6. response to osmotic stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Protein LEAF WILTING 3
Gene namesi
Name:ALG11
Synonyms:LEW3
Ordered Locus Names:At2g40190
ORF Names:T07M07.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G40190.

Subcellular locationi

Endoplasmic reticulum membrane; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Reviewed prediction
Add
BLAST
Transmembranei204 – 22623Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB
  2. endoplasmic reticulum membrane Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Lethality.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi148 – 1481G → E in lew3; deficiency in N-glycosylation leading to dwarf phenotype, reduced fertility and impaired cellulose synthesis. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 463463GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
PRO_0000412585Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi227 – 2271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi314 – 3141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi423 – 4231N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9XEE9.

Expressioni

Tissue specificityi

Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.1 Publication

Gene expression databases

GenevestigatoriQ9XEE9.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40190.1-P.

Structurei

3D structure databases

ProteinModelPortaliQ9XEE9.
SMRiQ9XEE9. Positions 272-442.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi46 – 494Poly-Gly

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0438.
HOGENOMiHOG000209670.
InParanoidiQ0WT32.
KOiK03844.
OMAiGTVILAH.

Family and domain databases

InterProiIPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q9XEE9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MAIYFILYTL LTIIFAVSLS LFLSVINARK SRKRAVGFFH PYTNDGGGGE    50
RVLWCAVKAI QEENPDLDCV IFTGDHDSSS DSLARRAVDR FGVHLQSPPK 100
VIHLNKRKWI EESTYPHFTM IGQSLGSVYL AWEALRMFTP LYFLDTSGYA 150
FTYPLARIFG CKVVCYTHYP TISLDMISRV RQRNSMYNND ASIAKSNWLS 200
TCKLVYYRAF SWMYGMVGSC THLAMVNSSW TKSHIEVLWR IPERITRVYP 250
PCDTSGLQAF PLERSSDPPK IISVAQFRPE KAHMLQLEAF SLALEKLDAD 300
VPRPKLQFVG SCRNNSDEER LQKLKDRAVE LKVDGDVQFY KNAMYRELVE 350
LLGNAVAGLH GMIDEHFGIS VVEYMAAGAI PIAHNSAGPK MDIVLEEDGQ 400
KTGFLAETVE EYAEAILEIV KMNETERLKM AESARKRAAR FSEQRFCEDF 450
KTAIRPIFTG PLK 463
Length:463
Mass (Da):52,573
Last modified:September 21, 2011 - v2
Checksum:i7092C34149CD4AE1
GO
Isoform 2 (identifier: Q9XEE9-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-212: Missing.

Show »
Length:251
Mass (Da):28,261
Checksum:iCFB26F285390374C
GO

Sequence cautioni

The sequence AAD25934.1 differs from that shown. Reason: Erroneous gene model prediction.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 212212Missing in isoform 2.
VSP_041714Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1.
AK227731 mRNA. Translation: BAE99716.1.
BT009712 mRNA. Translation: AAP88346.1.
PIRiD84826.
RefSeqiNP_181548.2. NM_129577.4. [Q9XEE9-1]
UniGeneiAt.21597.

Genome annotation databases

EnsemblPlantsiAT2G40190.1; AT2G40190.1; AT2G40190. [Q9XEE9-1]
GeneIDi818610.
KEGGiath:AT2G40190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1 .
AK227731 mRNA. Translation: BAE99716.1 .
BT009712 mRNA. Translation: AAP88346.1 .
PIRi D84826.
RefSeqi NP_181548.2. NM_129577.4. [Q9XEE9-1 ]
UniGenei At.21597.

3D structure databases

ProteinModelPortali Q9XEE9.
SMRi Q9XEE9. Positions 272-442.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G40190.1-P.

Protein family/group databases

CAZyi GT4. Glycosyltransferase Family 4.

Proteomic databases

PRIDEi Q9XEE9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G40190.1 ; AT2G40190.1 ; AT2G40190 . [Q9XEE9-1 ]
GeneIDi 818610.
KEGGi ath:AT2G40190.

Organism-specific databases

TAIRi AT2G40190.

Phylogenomic databases

eggNOGi COG0438.
HOGENOMi HOG000209670.
InParanoidi Q0WT32.
KOi K03844.
OMAi GTVILAH.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

PROi Q9XEE9.

Gene expression databases

Genevestigatori Q9XEE9.

Family and domain databases

InterProi IPR001296. Glyco_trans_1.
[Graphical view ]
Pfami PF00534. Glycos_transf_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07."
    Wang M.L., Belmonte S., Kim U., Dolan M., Morris J.W., Goodman H.M.
    Genome Res. 9:325-333(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Morosawa T., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K.
    , Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 284-463.
  6. "LEW3, encoding a putative alpha-1,2-mannosyltransferase (ALG11) in N-linked glycoprotein, plays vital roles in cell-wall biosynthesis and the abiotic stress response in Arabidopsis thaliana."
    Zhang M., Henquet M., Chen Z., Zhang H., Zhang Y., Ren X., van der Krol S., Gonneau M., Bosch D., Gong Z.
    Plant J. 60:983-999(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF GLY-148, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiALG11_ARATH
AccessioniPrimary (citable) accession number: Q9XEE9
Secondary accession number(s): F4IH01, Q0WT32, Q7DNH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: May 14, 2014
This is version 65 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG11 activity, no N-glycans are produced and transferred to proteins.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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