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Protein

GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase

Gene

ALG11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for N-linked oligosaccharide assembly. Has a role in the last step of the synthesis of the Man5GlcNAc(2)-PP-dolichol core oligosaccharide on the cytoplasmic face of the endoplasmic reticulum.1 Publication

Catalytic activityi

2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = 2 GDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol.1 Publication

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

  • GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity Source: UniProtKB

GO - Biological processi

  • oligosaccharide-lipid intermediate biosynthetic process Source: UniProtKB
  • plant-type cell wall biogenesis Source: TAIR
  • protein N-linked glycosylation Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to osmotic stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase (EC:2.4.1.131)
Alternative name(s):
Alpha-1,2-mannosyltransferase ALG11
Asparagine-linked glycosylation protein 11
Protein LEAF WILTING 3
Gene namesi
Name:ALG11
Synonyms:LEW3
Ordered Locus Names:At2g40190
ORF Names:T07M07.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40190.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21
Transmembranei204 – 226HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Lethality.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi148G → E in lew3; deficiency in N-glycosylation leading to dwarf phenotype, reduced fertility and impaired cellulose synthesis. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004125851 – 463GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferaseAdd BLAST463

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi227N-linked (GlcNAc...)Sequence analysis1
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi423N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ9XEE9.

Expressioni

Tissue specificityi

Expressed in shoots, leaves, sepals, filaments, siliques, stems, roots and guard cells.1 Publication

Gene expression databases

ExpressionAtlasiQ9XEE9. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G40190.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XEE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi46 – 49Poly-Gly4

Sequence similaritiesi

Belongs to the glycosyltransferase 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1387. Eukaryota.
COG0438. LUCA.
HOGENOMiHOG000209670.
InParanoidiQ9XEE9.
KOiK03844.
OMAiGTVILAH.
OrthoDBiEOG093608V7.

Family and domain databases

InterProiIPR031814. ALG11_N.
IPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF15924. ALG11_N. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9XEE9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAIYFILYTL LTIIFAVSLS LFLSVINARK SRKRAVGFFH PYTNDGGGGE
60 70 80 90 100
RVLWCAVKAI QEENPDLDCV IFTGDHDSSS DSLARRAVDR FGVHLQSPPK
110 120 130 140 150
VIHLNKRKWI EESTYPHFTM IGQSLGSVYL AWEALRMFTP LYFLDTSGYA
160 170 180 190 200
FTYPLARIFG CKVVCYTHYP TISLDMISRV RQRNSMYNND ASIAKSNWLS
210 220 230 240 250
TCKLVYYRAF SWMYGMVGSC THLAMVNSSW TKSHIEVLWR IPERITRVYP
260 270 280 290 300
PCDTSGLQAF PLERSSDPPK IISVAQFRPE KAHMLQLEAF SLALEKLDAD
310 320 330 340 350
VPRPKLQFVG SCRNNSDEER LQKLKDRAVE LKVDGDVQFY KNAMYRELVE
360 370 380 390 400
LLGNAVAGLH GMIDEHFGIS VVEYMAAGAI PIAHNSAGPK MDIVLEEDGQ
410 420 430 440 450
KTGFLAETVE EYAEAILEIV KMNETERLKM AESARKRAAR FSEQRFCEDF
460
KTAIRPIFTG PLK
Length:463
Mass (Da):52,573
Last modified:September 21, 2011 - v2
Checksum:i7092C34149CD4AE1
GO
Isoform 2 (identifier: Q9XEE9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-212: Missing.

Show »
Length:251
Mass (Da):28,261
Checksum:iCFB26F285390374C
GO

Sequence cautioni

The sequence AAD25934 differs from that shown. Reason: Erroneous gene model prediction.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0417141 – 212Missing in isoform 2. 1 PublicationAdd BLAST212

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1.
AK227731 mRNA. Translation: BAE99716.1.
BT009712 mRNA. Translation: AAP88346.1.
PIRiD84826.
RefSeqiNP_181548.2. NM_129577.5. [Q9XEE9-1]
UniGeneiAt.21597.

Genome annotation databases

EnsemblPlantsiAT2G40190.1; AT2G40190.1; AT2G40190. [Q9XEE9-1]
GeneIDi818610.
GrameneiAT2G40190.1; AT2G40190.1; AT2G40190.
KEGGiath:AT2G40190.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF085279 Genomic DNA. Translation: AAD25934.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09794.1.
AK227731 mRNA. Translation: BAE99716.1.
BT009712 mRNA. Translation: AAP88346.1.
PIRiD84826.
RefSeqiNP_181548.2. NM_129577.5. [Q9XEE9-1]
UniGeneiAt.21597.

3D structure databases

ProteinModelPortaliQ9XEE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G40190.1.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Proteomic databases

PaxDbiQ9XEE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40190.1; AT2G40190.1; AT2G40190. [Q9XEE9-1]
GeneIDi818610.
GrameneiAT2G40190.1; AT2G40190.1; AT2G40190.
KEGGiath:AT2G40190.

Organism-specific databases

TAIRiAT2G40190.

Phylogenomic databases

eggNOGiKOG1387. Eukaryota.
COG0438. LUCA.
HOGENOMiHOG000209670.
InParanoidiQ9XEE9.
KOiK03844.
OMAiGTVILAH.
OrthoDBiEOG093608V7.

Enzyme and pathway databases

UniPathwayiUPA00378.

Miscellaneous databases

PROiQ9XEE9.

Gene expression databases

ExpressionAtlasiQ9XEE9. baseline and differential.

Family and domain databases

InterProiIPR031814. ALG11_N.
IPR001296. Glyco_trans_1.
[Graphical view]
PfamiPF15924. ALG11_N. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALG11_ARATH
AccessioniPrimary (citable) accession number: Q9XEE9
Secondary accession number(s): F4IH01, Q0WT32, Q7DNH4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In the absence of ALG11 activity, no N-glycans are produced and transferred to proteins.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.