Q9XEE8 (P2C30_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable protein phosphatase 2C 30
Short name=AtPP2C30
EC=3.1.3.16

Alternative name(s):
AthPP2C5

Gene names

Name:	PP2C5
Ordered Locus Names:	At2g40180
ORF Names:	T7M7.17

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 2 magnesium or manganese ions per subunit By similarity.
Sequence similarities	Belongs to the PP2C family. Contains 1 PP2C-like domain.
Sequence caution	The sequence AAO42063.1 differs from that shown. Reason: Probable cloning artifact leading to a large internal deletion. The sequence AAO50609.1 differs from that shown. Reason: Probable cloning artifact leading to a large internal deletion.

Ontologies

Keywords
Ligand	Magnesium Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	abscisic acid mediated signaling pathway Inferred from mutant phenotype PubMed 20488890. Source: TAIR protein dephosphorylation Inferred from electronic annotation. Source: InterPro stomatal lineage progression Inferred from mutant phenotype PubMed 21203456. Source: TAIR
Cellular_component	nucleus Inferred from direct assay PubMed 20488890PubMed 21203456. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 20488890. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Probable protein phosphatase 2C 30		PRO_0000367958
Regions
	Domain	127 – 378	252	PP2C-like
Sites
	Metal binding	166	1	Manganese 1 By similarity
	Metal binding	166	1	Manganese 2 By similarity
	Metal binding	167	1	Manganese 1; via carbonyl oxygen By similarity
	Metal binding	331	1	Manganese 2 By similarity
	Metal binding	376	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XEE8 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F8FD06B046044D60	FASTA	390	42,595
	10 20 30 40 50 60 MQLSKNPIKQ TRNREKNYTD DFTMKRSVIM APESPVFFPP PLVFSPTSVK TPLSSPRSSP 70 80 90 100 110 120 PKLTMVACPP RKPKETKTTG SDSETVLKRK RPPMLDLTAA PTVASWCSTT RETAEKGAEV 130 140 150 160 170 180 VEAEEDGYYS VYCKRGRRGP MEDRYFAAVD RNDDGGYKNA FFGVFDGHGG SKAAEFAAMN 190 200 210 220 230 240 LGNNIEAAMA SARSGEDGCS MESAIREGYI KTDEDFLKEG SRGGACCVTA LISKGELAVS 250 260 270 280 290 300 NAGDCRAVMS RGGTAEALTS DHNPSQANEL KRIEALGGYV DCCNGVWRIQ GTLAVSRGIG 310 320 330 340 350 360 DRYLKEWVIA EPETRTLRIK PEFEFLILAS DGLWDKVTNQ EAVDVVRPYC VGVENPMTLS 370 380 390 ACKKLAELSV KRGSLDDISL IIIQLQNFLP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A cluster of ABA-regulated genes on Arabidopsis thaliana BAC T07M07." Wang M.L., Belmonte S., Kim U., Dolan M., Morris J.W., Goodman H.M. Genome Res. 9:325-333(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis." Xue T., Wang D., Zhang S., Ehlting J., Ni F., Jacab S., Zheng C., Zhong Y. BMC Genomics 9:550-550(2008) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF085279 Genomic DNA. Translation: AAD25933.1. CP002685 Genomic DNA. Translation: AEC09793.1. BT004027 mRNA. Translation: AAO42063.1. Sequence problems. BT005076 mRNA. Translation: AAO50609.1. Sequence problems.
IPI	IPI00525239.
PIR	C84826.
RefSeq	NP_181547.1. NM_129576.2.
UniGene	At.37092.
3D structure databases
HSSP	HSSP built from PDB template 1A6Q based on UniProtKB P35813.
ProteinModelPortal	Q9XEE8.
SMR	Q9XEE8. Positions 136-386.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G40180.1; AT2G40180.1; AT2G40180.
GeneID	818609.
KEGG	ath:AT2G40180.
Organism-specific databases
TAIR	At2g40180.
Phylogenomic databases
eggNOG	COG0631.
HOGENOM	HOG000233896.
InParanoid	Q9XEE8.
OMA	FEFLILA.
PhylomeDB	Q9XEE8.
ProtClustDB	CLSN2682545.
Gene expression databases
ArrayExpress	Q9XEE8.
Genevestigator	Q9XEE8.
Family and domain databases
Gene3D	3.60.40.10. 1 hit.
InterPro	IPR001932. PP2C-like. IPR000222. PP2C_Mn2_Asp60_BS. IPR015655. Protein_Pase_2C. [Graphical view]
PANTHER	PTHR13832. PTHR13832. 1 hit.
Pfam	PF00481. PP2C. 1 hit. [Graphical view]
SMART	SM00331. PP2C_SIG. 1 hit. SM00332. PP2Cc. 1 hit. [Graphical view]
SUPFAM	SSF81606. PP2C-related. 1 hit.
PROSITE	PS01032. PP2C. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

P2C30_ARATH

Accession

Primary (citable) accession number: Q9XEE8
Secondary accession number(s): Q84K48

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	November 1, 1999
Last modified:	May 1, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program