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Protein

Aspartic proteinase A3

Gene

APA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing and degradation of storage proteins.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei105PROSITE-ProRule annotation1
Active sitei292PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein catabolic process Source: GO_Central
  • proteolysis Source: GO_Central
  • response to cadmium ion Source: TAIR

Keywordsi

Molecular functionAspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

ReactomeiR-ATH-2132295. MHC class II antigen presentation.
R-ATH-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiA01.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase A3 (EC:3.4.23.-)
Gene namesi
Name:APA3
Ordered Locus Names:At4g04460
ORF Names:T26N6.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G04460.
TAIRilocus:2137189. AT4G04460.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
PropeptideiPRO_000042062926 – 69Activation peptideSequence analysisAdd BLAST44
ChainiPRO_000042063070 – 508Aspartic proteinase A3Add BLAST439

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi118 ↔ 124PROSITE-ProRule annotation
Disulfide bondi283 ↔ 287PROSITE-ProRule annotation
Disulfide bondi322 ↔ 413PROSITE-ProRule annotation
Disulfide bondi347 ↔ 385PROSITE-ProRule annotation
Disulfide bondi353 ↔ 382PROSITE-ProRule annotation
Glycosylationi399N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi427 ↔ 464PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9XEC4.
PRIDEiQ9XEC4.

Expressioni

Tissue specificityi

Expressed in petals, carpels and seed pods.1 Publication

Gene expression databases

ExpressionAtlasiQ9XEC4. baseline and differential.
GenevisibleiQ9XEC4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G04460.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XEC4.
SMRiQ9XEC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini87 – 505Peptidase A1PROSITE-ProRule annotationAdd BLAST419
Domaini317 – 419Saposin B-typePROSITE-ProRule annotationAdd BLAST103

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiQ9XEC4.
KOiK08245.
OMAiLDSQYFG.
OrthoDBiEOG093606Q0.
PhylomeDBiQ9XEC4.

Family and domain databases

CDDicd06098. phytepsin. 1 hit.
Gene3Di2.40.70.10. 2 hits.
InterProiView protein in InterPro
IPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR033869. Phytepsin.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
PANTHERiPTHR13683. PTHR13683. 1 hit.
PfamiView protein in Pfam
PF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiView protein in PROSITE
PS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: Q9XEC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTRFQSFLL VFLLSCLILI STASCERNGD GTIRIGLKKR KLDRSNRLAS
60 70 80 90 100
QLFLKNRGSH WSPKHYFRLN DENADMVPLK NYLDAQYYGD ITIGTPPQKF
110 120 130 140 150
TVIFDTGSSN LWIPSTKCYL SVACYFHSKY KASQSSSYRK NGKPASIRYG
160 170 180 190 200
TGAISGYFSN DDVKVGDIVV KEQEFIEATS EPGITFLLAK FDGILGLGFK
210 220 230 240 250
EISVGNSTPV WYNMVEKGLV KEPIFSFWLN RNPKDPEGGE IVFGGVDPKH
260 270 280 290 300
FKGEHTFVPV THKGYWQFDM GDLQIAGKPT GYCAKGCSAI ADSGTSLLTG
310 320 330 340 350
PSTVITMINH AIGAQGIVSR ECKAVVDQYG KTMLNSLLAQ EDPKKVCSQI
360 370 380 390 400
GVCAYDGTQS VSMGIQSVVD DGTSGLLNQA MCSACEMAAV WMESELTQNQ
410 420 430 440 450
TQERILAYAA ELCDHIPTQN QQSAVDCGRV SSMPIVTFSI GGRSFDLTPQ
460 470 480 490 500
DYIFKIGEGV ESQCTSGFTA MDIAPPRGPL WILGDIFMGP YHTVFDYGKG

RVGFAKAA
Length:508
Mass (Da):55,571
Last modified:November 1, 1999 - v1
Checksum:i2A4F1BC4F598F8F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076243 Genomic DNA. Translation: AAD29758.1.
AL161500 Genomic DNA. Translation: CAB77914.1.
CP002687 Genomic DNA. Translation: AEE82390.1.
AF372974 mRNA. Translation: AAK50111.1.
PIRiD85056.
RefSeqiNP_192355.1. NM_116684.4. [Q9XEC4-1]
UniGeneiAt.20045.
At.4020.

Genome annotation databases

EnsemblPlantsiAT4G04460.1; AT4G04460.1; AT4G04460. [Q9XEC4-1]
GeneIDi825776.
GrameneiAT4G04460.1; AT4G04460.1; AT4G04460.
KEGGiath:AT4G04460.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPA3_ARATH
AccessioniPrimary (citable) accession number: Q9XEC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1999
Last modified: August 30, 2017
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families