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Protein

Aspartic proteinase A3

Gene

APA3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing and degradation of storage proteins.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei105 – 1051PROSITE-ProRule annotation
Active sitei292 – 2921PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • lipid metabolic process Source: InterPro
  • protein catabolic process Source: GO_Central
  • proteolysis Source: GO_Central
  • response to cadmium ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl protease, Hydrolase, Protease

Enzyme and pathway databases

BioCyciARA:AT4G04460-MONOMER.
ARA:GQT-23-MONOMER.
ReactomeiR-ATH-2132295. MHC class II antigen presentation.

Protein family/group databases

MEROPSiA01.A03.

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartic proteinase A3 (EC:3.4.23.-)
Gene namesi
Name:APA3
Ordered Locus Names:At4g04460
ORF Names:T26N6.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G04460.

Subcellular locationi

  • Secreted 1 Publication

  • Note: Identified by mass spectrometry in phloem exudate.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Propeptidei26 – 6944Activation peptideSequence analysisPRO_0000420629Add
BLAST
Chaini70 – 508439Aspartic proteinase A3PRO_0000420630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi118 ↔ 124PROSITE-ProRule annotation
Disulfide bondi283 ↔ 287PROSITE-ProRule annotation
Disulfide bondi322 ↔ 413PROSITE-ProRule annotation
Disulfide bondi347 ↔ 385PROSITE-ProRule annotation
Disulfide bondi353 ↔ 382PROSITE-ProRule annotation
Glycosylationi399 – 3991N-linked (GlcNAc...)PROSITE-ProRule annotation
Disulfide bondi427 ↔ 464PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9XEC4.
PRIDEiQ9XEC4.

Expressioni

Tissue specificityi

Expressed in petals, carpels and seed pods.1 Publication

Gene expression databases

ExpressionAtlasiQ9XEC4. baseline and differential.
GenevisibleiQ9XEC4. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G04460.1.

Structurei

3D structure databases

ProteinModelPortaliQ9XEC4.
SMRiQ9XEC4. Positions 33-508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini87 – 505419Peptidase A1PROSITE-ProRule annotationAdd
BLAST
Domaini317 – 419103Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase A1 family.Curated
Contains 1 peptidase A1 domain.PROSITE-ProRule annotation
Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiQ9XEC4.
OMAiGYCAKGC.
PhylomeDBiQ9XEC4.

Family and domain databases

Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: Q9XEC4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGTRFQSFLL VFLLSCLILI STASCERNGD GTIRIGLKKR KLDRSNRLAS
60 70 80 90 100
QLFLKNRGSH WSPKHYFRLN DENADMVPLK NYLDAQYYGD ITIGTPPQKF
110 120 130 140 150
TVIFDTGSSN LWIPSTKCYL SVACYFHSKY KASQSSSYRK NGKPASIRYG
160 170 180 190 200
TGAISGYFSN DDVKVGDIVV KEQEFIEATS EPGITFLLAK FDGILGLGFK
210 220 230 240 250
EISVGNSTPV WYNMVEKGLV KEPIFSFWLN RNPKDPEGGE IVFGGVDPKH
260 270 280 290 300
FKGEHTFVPV THKGYWQFDM GDLQIAGKPT GYCAKGCSAI ADSGTSLLTG
310 320 330 340 350
PSTVITMINH AIGAQGIVSR ECKAVVDQYG KTMLNSLLAQ EDPKKVCSQI
360 370 380 390 400
GVCAYDGTQS VSMGIQSVVD DGTSGLLNQA MCSACEMAAV WMESELTQNQ
410 420 430 440 450
TQERILAYAA ELCDHIPTQN QQSAVDCGRV SSMPIVTFSI GGRSFDLTPQ
460 470 480 490 500
DYIFKIGEGV ESQCTSGFTA MDIAPPRGPL WILGDIFMGP YHTVFDYGKG

RVGFAKAA
Length:508
Mass (Da):55,571
Last modified:November 1, 1999 - v1
Checksum:i2A4F1BC4F598F8F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076243 Genomic DNA. Translation: AAD29758.1.
AL161500 Genomic DNA. Translation: CAB77914.1.
CP002687 Genomic DNA. Translation: AEE82390.1.
AF372974 mRNA. Translation: AAK50111.1.
PIRiD85056.
RefSeqiNP_192355.1. NM_116684.3. [Q9XEC4-1]
UniGeneiAt.20045.
At.4020.

Genome annotation databases

EnsemblPlantsiAT4G04460.1; AT4G04460.1; AT4G04460. [Q9XEC4-1]
GeneIDi825776.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF076243 Genomic DNA. Translation: AAD29758.1.
AL161500 Genomic DNA. Translation: CAB77914.1.
CP002687 Genomic DNA. Translation: AEE82390.1.
AF372974 mRNA. Translation: AAK50111.1.
PIRiD85056.
RefSeqiNP_192355.1. NM_116684.3. [Q9XEC4-1]
UniGeneiAt.20045.
At.4020.

3D structure databases

ProteinModelPortaliQ9XEC4.
SMRiQ9XEC4. Positions 33-508.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT4G04460.1.

Protein family/group databases

MEROPSiA01.A03.

Proteomic databases

PaxDbiQ9XEC4.
PRIDEiQ9XEC4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G04460.1; AT4G04460.1; AT4G04460. [Q9XEC4-1]
GeneIDi825776.

Organism-specific databases

TAIRiAT4G04460.

Phylogenomic databases

eggNOGiKOG1339. Eukaryota.
ENOG410XNV7. LUCA.
HOGENOMiHOG000197681.
InParanoidiQ9XEC4.
OMAiGYCAKGC.
PhylomeDBiQ9XEC4.

Enzyme and pathway databases

BioCyciARA:AT4G04460-MONOMER.
ARA:GQT-23-MONOMER.
ReactomeiR-ATH-2132295. MHC class II antigen presentation.

Miscellaneous databases

PROiQ9XEC4.

Gene expression databases

ExpressionAtlasiQ9XEC4. baseline and differential.
GenevisibleiQ9XEC4. AT.

Family and domain databases

Gene3Di1.10.225.10. 2 hits.
2.40.70.10. 3 hits.
InterProiIPR001461. Aspartic_peptidase_A1.
IPR001969. Aspartic_peptidase_AS.
IPR033121. PEPTIDASE_A1.
IPR021109. Peptidase_aspartic_dom.
IPR007856. SapB_1.
IPR008138. SapB_2.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PANTHERiPTHR13683. PTHR13683. 2 hits.
PfamiPF00026. Asp. 1 hit.
PF05184. SapB_1. 1 hit.
PF03489. SapB_2. 1 hit.
[Graphical view]
PRINTSiPR00792. PEPSIN.
SUPFAMiSSF47862. SSF47862. 1 hit.
SSF50630. SSF50630. 2 hits.
PROSITEiPS00141. ASP_PROTEASE. 2 hits.
PS51767. PEPTIDASE_A1. 1 hit.
PS50015. SAP_B. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed."
    Chen X., Pfeil J.E., Gal S.
    Eur. J. Biochem. 269:4675-4684(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. "Identification of lipids and lipid-binding proteins in phloem exudates from Arabidopsis thaliana."
    Guelette B.S., Benning U.F., Hoffmann-Benning S.
    J. Exp. Bot. 63:3603-3616(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiAPA3_ARATH
AccessioniPrimary (citable) accession number: Q9XEC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.