Reviewed, UniProtKB/Swiss-Prot Q9XEA8 (CYSK2_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	RCS3
Ordered Locus Names:	Os03g0747800, LOC_Os03g53650
ORF Names:	OJ1124_H03.2, OSJNBa0069E14.1

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.
Sequence caution	The sequence AAL58961.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Cysteine synthase		PRO_0000167122
Regions
	Region	184 – 188	5	Pyridoxal phosphate binding By similarity
	Compositional bias	275 – 280	6	Poly-Ala
Sites
	Binding site	80	1	Pyridoxal phosphate By similarity
	Binding site	272	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	49	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XEA8-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8F6B0CDCCD7DCAC4	FASTA	325	34,307
	10 20 30 40 50 60 MAESGQSIAS DVTALIGNTP LVYLNKVVDG CEAQIAAKLE IMEPCSSVKD RIGYSMITDA 70 80 90 100 110 120 EEKGLITPGK SVLIEPTSGN TGIGLAFMAA AKGYKLILTM PASMSMERRI ILKAFGAELV 130 140 150 160 170 180 LTDPLLGMKG AIQKADELAA KMPNSYILQQ FENPANPKIH YETTGPEIWK ATAGKVDILV 190 200 210 220 230 240 SGIGTGGTVT GTGKYLKEQN PEIKIYGVEP TESAILSGGR PGPHKIQGIG AGFVPGVLDV 250 260 270 280 290 300 NLLDEVVQVS SDEAISMAKQ LALKEGLLVG ISSGAAAVAA IRVAQRPENK GKLVVVVFPS 310 320 FGERYLSSVL FESIKREAEN MVFEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four rice genes encoding cysteine synthase: isolation and differential responses to sulfur, nitrogen and light." Nakamura T., Yamaguchi Y., Sano H. Gene 229:155-161(1999) [PubMed: 10095115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"Sequence, annotation, and analysis of synteny between rice chromosome 3 and diverged grass species." The rice chromosome 3 sequencing consortium Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B., Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T., Fadrosh D., Bera J., Weaver B., Jin S. , Johri S., Reardon M., Webb K., Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T., Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R., Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J., Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S., Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M., Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M., Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L., O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R., Jin W., Lee H.R., Jiang J., Jackson S. Genome Res. 15:1284-1291(2005) [PubMed: 16109971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073697 mRNA. Translation: AAD23909.1. AC087852 Genomic DNA. Translation: AAK71541.1. AC091811 Genomic DNA. Translation: AAL58961.1. Sequence problems.
RefSeq	NP_001051262.1.
UniGene	Os.7102
3D structure databases
HSSP	HSSP built from PDB template 1FCJ based on UniProtKB P12674.
SMR	Q9XEA8. Positions 8-324.
ModBase	Search...
2-D gel databases
ANU-2DPAGE	Q9XEA8.
Genome annotation databases
GeneID	4334101.
KEGG	osa:4334101.
Organism-specific databases
Gramene	Q9XEA8.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK2_ORYSJ

Accession

Primary (citable) accession number: Q9XEA8
Secondary accession number(s): Q7G7J5, Q8W313

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	July 7, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)