Q9XEA6 (CYSK1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine synthase
Short name=CSase
EC=2.5.1.47

Alternative name(s):
O-acetylserine (thiol)-lyase
Short name=OAS-TL
O-acetylserine sulfhydrylase

Gene names

Name:	RCS1
Ordered Locus Names:	Os12g0625000, LOC_Os12g42980

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Cysteine synthase		PRO_0000167121
Regions
	Region	181 – 185	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	77	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XEA6 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 9DCC3A2D961C7FD6	FASTA	321	33,838
	10 20 30 40 50 60 MGETIAKDVT ELIGNTPLVY LNRVTDGCVG RVAAKLESME PCSSVKDRIG YSMITDAEEK 70 80 90 100 110 120 GLITPGKSVL IEPTSGNTGI GLAFMAAAKG YRLVLTMPAS MSMERRIILK AFGAELILTD 130 140 150 160 170 180 PLLGMKGAVQ KAEELAAKTN NSFILQQFEN PANPKIHYET TGPEIWKGTG GKVDGLVSGI 190 200 210 220 230 240 GTGGTITGAG RYLREQNPDI KIYGVEPVES AVLSGGKPGP HKIQGIGAGF VPGVLDVDLI 250 260 270 280 290 300 NETVQVSSDE AIEMAKALAL KEGLLVGISS GAAAAAAVRL AQRPENEGKL FVVVFPSFGE 310 320 RYLSSVLFQS IKKEAENMVV E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four rice genes encoding cysteine synthase: isolation and differential responses to sulfur, nitrogen and light." Nakamura T., Yamaguchi Y., Sano H. Gene 229:155-161(1999) [PubMed: 10095115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"The rice annotation project database (RAP-DB): 2008 update." The rice annotation project (RAP) Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed: 18089549] [Abstract] Cited for: GENOME REANNOTATION. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF073695 mRNA. Translation: AAD23907.1. AP008218 Genomic DNA. Translation: BAF30338.1.
RefSeq	NP_001067319.1. NM_001073851.1.
UniGene	Os.2258.
3D structure databases
ProteinModelPortal	Q9XEA6.
SMR	Q9XEA6. Positions 5-321.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9XEA6.
Proteomic databases
PRIDE	Q9XEA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4352839.
KEGG	osa:4352839.
Organism-specific databases
Gramene	Q9XEA6.
Phylogenomic databases
GeneTree	EPGT00050000010053.
PhylomeDB	Q9XEA6.
ProtClustDB	PLN02565.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
KO	K01738.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR01139. CysK. 1 hit. TIGR01136. CysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK1_ORYSJ

Accession

Primary (citable) accession number: Q9XEA6
Secondary accession number(s): Q0ILS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 20, 2005
Last modified:	November 16, 2011
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program