Reviewed, UniProtKB/Swiss-Prot Q9XEA6 (CYSK1_ORYSJ)

Names and origin

Protein names

Recommended name:
    Cysteine synthase
      Short name=CSase
    EC=2.5.1.47
Alternative name(s):
    O-acetylserine sulfhydrylase
    O-acetylserine (thiol)-lyase
      Short name=OAS-TL

Gene names

Name:	RCS1
Ordered Locus Names:	Os12g0625000, LOC_Os12g42980

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	321 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 321	321	Cysteine synthase		PRO_0000167121
Regions
	Region	181 – 185	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	77	1	Pyridoxal phosphate By similarity
	Binding site	269	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	46	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XEA6-1 [UniParc]. Last modified December 20, 2005. Version 2. Checksum: 9DCC3A2D961C7FD6	FASTA	321	33,838
	10 20 30 40 50 60 MGETIAKDVT ELIGNTPLVY LNRVTDGCVG RVAAKLESME PCSSVKDRIG YSMITDAEEK 70 80 90 100 110 120 GLITPGKSVL IEPTSGNTGI GLAFMAAAKG YRLVLTMPAS MSMERRIILK AFGAELILTD 130 140 150 160 170 180 PLLGMKGAVQ KAEELAAKTN NSFILQQFEN PANPKIHYET TGPEIWKGTG GKVDGLVSGI 190 200 210 220 230 240 GTGGTITGAG RYLREQNPDI KIYGVEPVES AVLSGGKPGP HKIQGIGAGF VPGVLDVDLI 250 260 270 280 290 300 NETVQVSSDE AIEMAKALAL KEGLLVGISS GAAAAAAVRL AQRPENEGKL FVVVFPSFGE 310 320 RYLSSVLFQS IKKEAENMVV E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Four rice genes encoding cysteine synthase: isolation and differential responses to sulfur, nitrogen and light." Nakamura T., Yamaguchi Y., Sano H. Gene 229:155-161(1999) [PubMed: 10095115] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF073695 mRNA. Translation: AAD23907.1. BX000561 Genomic DNA. No translation available.
RefSeq	NP_001067319.1.
UniGene	Os.2258
3D structure databases
HSSP	HSSP built from PDB template 1FCJ based on UniProtKB P12674.
SMR	Q9XEA6. Positions 5-321.
ModBase	Search...
Genome annotation databases
GeneID	4352839.
KEGG	osa:4352839.
Organism-specific databases
Gramene	Q9XEA6.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSK1_ORYSJ

Accession

Primary (citable) accession number: Q9XEA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 20, 2005
Last modified:	June 16, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)