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Protein

Leucine--tRNA ligase, chloroplastic/mitochondrial

Gene

EMB2369

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a two step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA.By similarity

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei733ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • leucyl-tRNA aminoacylation Source: GO_Central
  • mitochondrial translation Source: GO_Central

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligase, chloroplastic/mitochondrialCurated (EC:6.1.1.4Curated)
Alternative name(s):
Leucyl-tRNA synthetaseCurated
Short name:
LeuRSCurated
Protein EMBRYO DEFECTIVE 23691 Publication
Gene namesi
Name:EMB23691 Publication
Ordered Locus Names:At4g04350Imported
ORF Names:T19B17.7Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G04350
TAIRilocus:2134887 AT4G04350

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Disruption phenotypei

Embryo defective. Developmental arrest of the embryo at the globular stage.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000433548? – 973Leucine--tRNA ligase, chloroplastic/mitochondrialCurated
Transit peptidei1 – ?Chloroplast and mitochondrionCurated

Proteomic databases

PaxDbiQ9XEA0
PRIDEiQ9XEA0

Expressioni

Gene expression databases

ExpressionAtlasiQ9XEA0 baseline and differential
GenevisibleiQ9XEA0 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G04350.1

Structurei

3D structure databases

ProteinModelPortaliQ9XEA0
SMRiQ9XEA0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi126 – 135"HIGH" regionCurated10
Motifi730 – 734"KMSKS" regionCurated5

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0435 Eukaryota
COG0495 LUCA
HOGENOMiHOG000200748
InParanoidiQ9XEA0
KOiK01869
OMAiTFMVLAP
OrthoDBiEOG093601DH
PhylomeDBiQ9XEA0

Family and domain databases

Gene3Di3.40.50.620, 3 hits
3.90.740.10, 1 hit
HAMAPiMF_00049_B Leu_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR002302 Leu-tRNA-ligase
IPR025709 Leu_tRNA-synth_edit
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
PANTHERiPTHR43740 PTHR43740, 1 hit
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF13603 tRNA-synt_1_2, 1 hit
PF09334 tRNA-synt_1g, 1 hit
PRINTSiPR00985 TRNASYNTHLEU
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00396 leuS_bact, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XEA0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSHHQILQI RSDPFVLSHC CRHTRLTSSL TLQSPLKQPF SCLPFRWRRS
60 70 80 90 100
YRGGVRSSTT ETHGSKKEAL VSETATTSIE LKRVYPFHEI EPKWQRYWED
110 120 130 140 150
NRIFRTPDDV DTSKPKFYVL DMFPYPSGAG LHVGHPLGYT ATDILARLRR
160 170 180 190 200
MQGYNVLHPM GWDAFGLPAE QYAIETGTHP KTTTLKNIDR FRLQLKSLGF
210 220 230 240 250
SYDWDRELST TEPDYYKWTQ WIFLQLYKKG LAYQAEVPVN WCPALGTVLA
260 270 280 290 300
NEEVVDGVSE RGGHPVIRKP MRQWMLKITA YADRLLEDLD ELEWPESIKE
310 320 330 340 350
MQRNWIGRSE GAELNFSILD GEGRETDKEI TVYTTRPDTL FGATYMVVAP
360 370 380 390 400
EHQLLSYFVT AEQKQQVEEY KDFASRKSDL ERTELQKDKT GVFTGCYAKN
410 420 430 440 450
PANGDAIPIW VADYVLASYG TGAIMAVPAH DTRDNEFALK YNIPIKWVVR
460 470 480 490 500
NEANSSDDAK QVYPGLGIIE NSSTLETGLD INQLSSKEAA LKVIEWAERT
510 520 530 540 550
GNGKKKVNYK LRDWLFARQR YWGEPIPILI LDESGETIAI SESELPLTLP
560 570 580 590 600
ELNDFTPTGT GEPPLSKAVS WVNTVDPSTG KPAKRETSTM PQWAGSCWYY
610 620 630 640 650
LRFMDPKNPE ALVDKEKEKY WSPVDVYVGG AEHAVLHLLY SRFWHKVLYD
660 670 680 690 700
IGVVSTKEPF KCVINQGIIL GEVQYTAWKD QEGNYVSADT EERLNEHQQV
710 720 730 740 750
TIPEEKVIKS GDHFVLKEDP SIRLIPRVYK MSKSRGNVVN PDDVVLEYGA
760 770 780 790 800
DSLRLYEMFM GPFRDSKTWN TSGIEGVHRF LARTWRLVIG LPQSDGSFKD
810 820 830 840 850
GTLVTDDEPT LEQLRTLHKC IAKVTEEIES TRFNTGISGM MEFVNAAYKW
860 870 880 890 900
NNQPRGIIEP FVLLLSPYAP HMAEELWSRL GHPNSLAYES FPKANPDYLK
910 920 930 940 950
NTTIVLPVQI NGKTRGTIEV EEGCSEDDAF VLASQDDKLR KYLDGQSIKK
960 970
RIYVPGKILN VILDRTNVKV TTK
Length:973
Mass (Da):111,033
Last modified:November 1, 1999 - v1
Checksum:i286C9807D33D93F2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF069441 Genomic DNA Translation: AAD36946.1
AL161500 Genomic DNA Translation: CAB77903.1
CP002687 Genomic DNA Translation: AEE82383.1
AY079159 mRNA Translation: AAL84999.1
BT002249 mRNA Translation: AAN72260.1
PIRiA85055
RefSeqiNP_192344.1, NM_116673.7
UniGeneiAt.27749
At.74904

Genome annotation databases

EnsemblPlantsiAT4G04350.1; AT4G04350.1; AT4G04350
GeneIDi825755
GrameneiAT4G04350.1; AT4G04350.1; AT4G04350
KEGGiath:AT4G04350

Similar proteinsi

Entry informationi

Entry nameiSYLM_ARATH
AccessioniPrimary (citable) accession number: Q9XEA0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 22, 2015
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

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