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Q9XDB4

- SYI_FERPD

UniProt

Q9XDB4 - SYI_FERPD

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Ferpe_1791
Organism
Fervidobacterium pennivorans (strain DSM 9078 / Ven5)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei549 – 5491Aminoacyl-adenylate By similarity
Binding sitei593 – 5931ATP By similarity
Metal bindingi880 – 8801Zinc By similarity
Metal bindingi883 – 8831Zinc By similarity
Metal bindingi900 – 9001Zinc By similarity
Metal bindingi903 – 9031Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciFPEN771875:GLDA-1791-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Ferpe_1791
OrganismiFervidobacterium pennivorans (strain DSM 9078 / Ven5)
Taxonomic identifieri771875 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaeFervidobacterium
ProteomesiUP000007384: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 912912Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098387Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi590 – 5945"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

KOiK01870.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XDB4-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKATLNLP QTNFQMKANL VNKEPEMLKF WEEKEIYKKT LETRANAPTY    50
LLHDGPPYAN GDIHLGTAMN KILKDFVTRY KTMRGYRVPF VPGWDTHGLP 100
IEHRVTTSLG EEAKKKSPAE IRKLCKEFAL KYVDIQREEF KRLGVKGDWE 150
HPYITLDPDY EYHILDVFKT LVENGNVYRG NKPVYWCPTC RTALAEAEIE 200
YHDHESPSIY VKFQMVDKPD TYIVIWTTTP WTIPANVAIA LHPDYTYVKI 250
KVDEEYWIVA EGLLQKFAAD VGIDFEVVEK FVGKELEGKL TKHPLYDRTS 300
VVVLADYVTL EDGTGCVHTA PGHGEEDYQT GLKYNLPVLS PVDDEGRFTK 350
EAGKYEGLKI WDANKVIVED LKNNGSLIKA GKISHSYPHC WRCKGPVMFR 400
ATPQWFISVD KNNLRGKVLE EIKKVKWYPA WGETRITAMV QERPDWTISR 450
QRVWGVPIPA VKCKDCGEVT LEPKVIEHFA NIVKEKGTDA WFELDVNELI 500
PADFKCPACG SKNFEKTHDT LDVWIDSGCS WEAVIRSKGE RFPVDLYLEG 550
DDQHRGWFQS SIFLSTAKAG TAPFKAVVTH GFIKDEQGRK MSKSLGNVID 600
PMEIVNKYGA DILRLWVAST DFFDNIRVGK NIIEQQVEVY RKLRNTLRYL 650
LSNLYDFTEA DLLPYEKLLP LDKWALGRLQ KFIEQITQYY EGFEYSKVYN 700
ATVKYCTTEL SAVYLDILKD RLYVEAKDSI YRRSAQTALH YILEALIKIL 750
APIIPFTAEE AYQESHLKRY ESVHLEYWPE VRKEFIDEAL LEEFDHLLLI 800
RDDVLKALEN ARASDIIGHS LDAHVIIEAK NEELKNLLRK YESLLEEFFI 850
VSKVTLSENI SGLNGQFANV LVQRAEGQKC QRCWKYHPDT GKDLEHPETC 900
PRCSAVLRGE RK 912
Length:912
Mass (Da):105,259
Last modified:September 5, 2012 - v3
Checksum:iA0C8FA7C5CE20B7E
GO

Sequence cautioni

The sequence AAD30388.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti525 – 5251I → M in AAD30388. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF096862 Genomic DNA. Translation: AAD30388.1. Different initiation.
CP003260 Genomic DNA. Translation: AFG35844.1.
RefSeqiYP_005471963.1. NC_017095.1.

Genome annotation databases

EnsemblBacteriaiAFG35844; AFG35844; Ferpe_1791.
GeneIDi12058543.
KEGGifpe:Ferpe_1791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF096862 Genomic DNA. Translation: AAD30388.1 . Different initiation.
CP003260 Genomic DNA. Translation: AFG35844.1 .
RefSeqi YP_005471963.1. NC_017095.1.

3D structure databases

ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AFG35844 ; AFG35844 ; Ferpe_1791 .
GeneIDi 12058543.
KEGGi fpe:Ferpe_1791.

Phylogenomic databases

KOi K01870.

Enzyme and pathway databases

BioCyci FPEN771875:GLDA-1791-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pullulanase type I from fervidobacterium pennavorans ven5: cloning, sequencing, and expression of the gene and biochemical characterization of the recombinant enzyme."
    Bertoldo C., Duffner F., Jorgensen P.L., Antranikian G.
    Appl. Environ. Microbiol. 65:2084-2091(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 9078 / Ven5.
  2. "Complete sequence of Fervidobacterium pennivorans DSM 9078."
    Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Ovchinnikova G., Lu M., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Noll K.M., Woyke T.
    Submitted (MAR-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 9078 / Ven5.

Entry informationi

Entry nameiSYI_FERPD
AccessioniPrimary (citable) accession number: Q9XDB4
Secondary accession number(s): H9UEA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: September 5, 2012
Last modified: April 16, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi