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Protein

Adhesin/invasin TibA autotransporter

Gene

tibA

Organism
Escherichia coli O78:H11 (strain H10407 / ETEC)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates both adhesion to and invasion of human intestine epithelial cells. Also mediates bacterial cell aggregation via intercellular TibA-TibA interaction. Enhances biofilm formation.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Virulence

Protein family/group databases

TCDBi1.B.12.8.3. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesin/invasin TibA autotransporter
Cleaved into the following 2 chains:
Alternative name(s):
Glycoprotein TibA
Gene namesi
Name:tibA
Ordered Locus Names:ETEC_2141
OrganismiEscherichia coli O78:H11 (strain H10407 / ETEC)
Taxonomic identifieri316401 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000006877 Componenti: Chromosome

Subcellular locationi

Adhesin/invasin TibA translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 542 PublicationsAdd BLAST54
ChainiPRO_000038758455 – 989Adhesin/invasin TibA autotransporterAdd BLAST935
ChainiPRO_000004460755 – 677Adhesin/invasin TibAAdd BLAST623
ChainiPRO_0000044608678 – 989Adhesin/invasin TibA translocatorAdd BLAST312

Post-translational modificationi

Glycosylated by TibC. Glycosylation is required for TibA-mediated adhesion and invasion of human cells but not for aggregation.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9XD84.

Structurei

Secondary structure

1989
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi56 – 59Combined sources4
Beta strandi61 – 63Combined sources3
Beta strandi69 – 73Combined sources5
Beta strandi78 – 81Combined sources4
Beta strandi89 – 92Combined sources4
Beta strandi97 – 100Combined sources4
Beta strandi108 – 111Combined sources4
Beta strandi116 – 119Combined sources4
Beta strandi127 – 130Combined sources4
Beta strandi135 – 138Combined sources4
Beta strandi146 – 149Combined sources4
Beta strandi154 – 157Combined sources4
Beta strandi165 – 169Combined sources5
Beta strandi173 – 176Combined sources4
Beta strandi184 – 188Combined sources5
Beta strandi192 – 195Combined sources4
Beta strandi203 – 206Combined sources4
Beta strandi211 – 214Combined sources4
Beta strandi222 – 226Combined sources5
Beta strandi230 – 233Combined sources4
Beta strandi241 – 246Combined sources6
Beta strandi248 – 254Combined sources7
Beta strandi259 – 262Combined sources4
Beta strandi267 – 273Combined sources7
Beta strandi278 – 281Combined sources4
Beta strandi286 – 292Combined sources7
Beta strandi297 – 300Combined sources4
Beta strandi305 – 311Combined sources7
Beta strandi316 – 318Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q1QX-ray2.11A/B55-350[»]
ProteinModelPortaliQ9XD84.
SMRiQ9XD84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati82 – 1001-1Add BLAST19
Repeati101 – 1191-2Add BLAST19
Repeati120 – 1381-3Add BLAST19
Repeati139 – 1571-4Add BLAST19
Repeati158 – 1761-5Add BLAST19
Repeati177 – 1951-6Add BLAST19
Repeati196 – 2141-7Add BLAST19
Repeati215 – 2331-8Add BLAST19
Repeati234 – 2511-9Add BLAST18
Repeati252 – 2701-10Add BLAST19
Repeati271 – 2891-11Add BLAST19
Repeati290 – 3081-12Add BLAST19
Repeati639 – 6432-15
Repeati644 – 6482-25
Repeati649 – 6532-35
Repeati654 – 6582-45
Repeati659 – 6632-55
Repeati664 – 6682-65
Repeati669 – 6732-75
Repeati674 – 6782-85
Domaini721 – 989AutotransporterPROSITE-ProRule annotationAdd BLAST269

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 30812 X 19 AA approximate repeatsAdd BLAST227
Regioni639 – 6788 X 5 AA repeats of P-[DG]-[AGT]-[DGA]-[NKT]Add BLAST40

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi87 – 447Gly-richAdd BLAST361

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

OMAiNDDARSH.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF16168. AIDA. 8 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
TIGR04415. O_hepto_targRPT. 7 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XD84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVYNTVWN ESTGTWVVTS ELTRKGGLRP RQIKRTVLAG LIAGLLMPSM
60 70 80 90 100
PALAAAYDNQ TIGRGETSKS MHLSAGDTAK NTTINSGGKQ YVSSGGSATS
110 120 130 140 150
TTINIGGVQH VSSGGSATSS TINSGGHQHV SSGGSATNTT VNNGGRQTVF
160 170 180 190 200
SGGSAMGTII NSGGDQYVIS GGSATSASVT SGARQFVSSG GIVKATSVNS
210 220 230 240 250
GGRQYVRDGG SATDTVLNNT GRQFVSSGGS AAKTTINSGG GMYLYGGSAT
260 270 280 290 300
GTSIYNGGRQ YVSSGGSATN TTVYSGGRQH VYIDGNVTET TITSGGMLQV
310 320 330 340 350
EAGGSASKVI QNSGGAVITN TSAAVSGTND NGSFSIAGGS AVNMLLENGG
360 370 380 390 400
YLTVFDGHQA SDTMVGSDGT LDVRSGGVLY GTTTLTDKGA LVGDVVTNEG
410 420 430 440 450
NLYYLNNSTA TFTGTLTGTG TLTQEGGNTR FSGLLSQDGG IFLQSGGAMT
460 470 480 490 500
MDALQAKANV TTQSGTTLTL DNGTILTGNV AGDSTGAGDM AVKGASVWHL
510 520 530 540 550
DGDSTVGALT LDNGTVDFRP STTTRMTPAF QAVSLALGSL SGSGTFQMNT
560 570 580 590 600
DIASHTGDML NVAGNASGNF VLDIKNTGLE PVSAGAPLQV VQTGGGDAAF
610 620 630 640 650
TLKGGKVDAG TWEYGLSKEN TNWYLKADTP PPVTPPTNPD ADNPDAGNPD
660 670 680 690 700
AGNPDAGNPD AGNPDAGKPG TGKPDAGTSS SPVRRTTKSV DAVLGMATAP
710 720 730 740 750
AYVFNSELDN LRFRHGDVMQ NTRAPGGVWG RYTGSDNRIS GGASSGYTLT
760 770 780 790 800
QNGFETGADM VFDLSDSSLA VGTFFSYSDN SIKHARGGKS NVDSSGGGLY
810 820 830 840 850
ATWFDNDGYY VDGVLKYNRF NNELRTWMSD GTAVKGDYSQ NGFGGSLEAG
860 870 880 890 900
RTFSLNENAW AQPYVRTTAF RADKKEIRLN NGMKASIGAT KSLQAEAGLK
910 920 930 940 950
LGMTLDVAGK EVKPYLSAAV SHEFSDNNKV RINDTYDFRN DISGTTGKYG
960 970 980
LGVNAQLTPN AGVWAEARYE NGKQTESPIT GGVGFRINF
Length:989
Mass (Da):101,112
Last modified:November 1, 1999 - v1
Checksum:i6A925466416E63BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109215 Genomic DNA. Translation: AAD41751.1.
FN649414 Genomic DNA. Translation: CBJ01643.1.
RefSeqiWP_001045641.1. NC_017633.1.

Genome annotation databases

EnsemblBacteriaiCBJ01643; CBJ01643; ETEC_2141.
KEGGielh:ETEC_2141.
PATRICi42960174. VBIEscCol136115_2454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109215 Genomic DNA. Translation: AAD41751.1.
FN649414 Genomic DNA. Translation: CBJ01643.1.
RefSeqiWP_001045641.1. NC_017633.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Q1QX-ray2.11A/B55-350[»]
ProteinModelPortaliQ9XD84.
SMRiQ9XD84.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.B.12.8.3. the autotransporter-1 (at-1) family.

Proteomic databases

PRIDEiQ9XD84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBJ01643; CBJ01643; ETEC_2141.
KEGGielh:ETEC_2141.
PATRICi42960174. VBIEscCol136115_2454.

Phylogenomic databases

OMAiNDDARSH.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF16168. AIDA. 8 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
TIGR04415. O_hepto_targRPT. 7 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIBA_ECOH1
AccessioniPrimary (citable) accession number: Q9XD84
Secondary accession number(s): E3PBM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

TibA-mediated aggregation is blocked upon capsule expression and fimbriation and is sensitive to pH extremes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.