Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Adhesin/invasin TibA autotransporter

Gene

tibA

Organism
Escherichia coli O78:H11 (strain H10407 / ETEC)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates both adhesion to and invasion of human intestine epithelial cells. Also mediates bacterial cell aggregation via intercellular TibA-TibA interaction. Enhances biofilm formation.2 Publications

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion, Virulence

Enzyme and pathway databases

BioCyciECOL316401:GLD0-2181-MONOMER.

Protein family/group databases

TCDBi1.B.12.8.3. the autotransporter-1 (at-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Adhesin/invasin TibA autotransporter
Cleaved into the following 2 chains:
Alternative name(s):
Glycoprotein TibA
Gene namesi
Name:tibA
Ordered Locus Names:ETEC_2141
OrganismiEscherichia coli O78:H11 (strain H10407 / ETEC)
Taxonomic identifieri316401 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000006877 Componenti: Chromosome

Subcellular locationi

Adhesin/invasin TibA translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 54542 PublicationsAdd
BLAST
Chaini55 – 989935Adhesin/invasin TibA autotransporterPRO_0000387584Add
BLAST
Chaini55 – 677623Adhesin/invasin TibAPRO_0000044607Add
BLAST
Chaini678 – 989312Adhesin/invasin TibA translocatorPRO_0000044608Add
BLAST

Post-translational modificationi

Glycosylated by TibC. Glycosylation is required for TibA-mediated adhesion and invasion of human cells but not for aggregation.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ9XD84.

Structurei

Secondary structure

1
989
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 594Combined sources
Beta strandi61 – 633Combined sources
Beta strandi69 – 735Combined sources
Beta strandi78 – 814Combined sources
Beta strandi89 – 924Combined sources
Beta strandi97 – 1004Combined sources
Beta strandi108 – 1114Combined sources
Beta strandi116 – 1194Combined sources
Beta strandi127 – 1304Combined sources
Beta strandi135 – 1384Combined sources
Beta strandi146 – 1494Combined sources
Beta strandi154 – 1574Combined sources
Beta strandi165 – 1695Combined sources
Beta strandi173 – 1764Combined sources
Beta strandi184 – 1885Combined sources
Beta strandi192 – 1954Combined sources
Beta strandi203 – 2064Combined sources
Beta strandi211 – 2144Combined sources
Beta strandi222 – 2265Combined sources
Beta strandi230 – 2334Combined sources
Beta strandi241 – 2466Combined sources
Beta strandi248 – 2547Combined sources
Beta strandi259 – 2624Combined sources
Beta strandi267 – 2737Combined sources
Beta strandi278 – 2814Combined sources
Beta strandi286 – 2927Combined sources
Beta strandi297 – 3004Combined sources
Beta strandi305 – 3117Combined sources
Beta strandi316 – 3183Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Q1QX-ray2.11A/B55-350[»]
ProteinModelPortaliQ9XD84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati82 – 100191-1Add
BLAST
Repeati101 – 119191-2Add
BLAST
Repeati120 – 138191-3Add
BLAST
Repeati139 – 157191-4Add
BLAST
Repeati158 – 176191-5Add
BLAST
Repeati177 – 195191-6Add
BLAST
Repeati196 – 214191-7Add
BLAST
Repeati215 – 233191-8Add
BLAST
Repeati234 – 251181-9Add
BLAST
Repeati252 – 270191-10Add
BLAST
Repeati271 – 289191-11Add
BLAST
Repeati290 – 308191-12Add
BLAST
Repeati639 – 64352-1
Repeati644 – 64852-2
Repeati649 – 65352-3
Repeati654 – 65852-4
Repeati659 – 66352-5
Repeati664 – 66852-6
Repeati669 – 67352-7
Repeati674 – 67852-8
Domaini721 – 989269AutotransporterPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni82 – 30822712 X 19 AA approximate repeatsAdd
BLAST
Regioni639 – 678408 X 5 AA repeats of P-[DG]-[AGT]-[DGA]-[NKT]Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi87 – 447361Gly-richAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

OMAiNDDARSH.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF16168. AIDA. 8 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
TIGR04415. O_hepto_targRPT. 7 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9XD84-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVYNTVWN ESTGTWVVTS ELTRKGGLRP RQIKRTVLAG LIAGLLMPSM
60 70 80 90 100
PALAAAYDNQ TIGRGETSKS MHLSAGDTAK NTTINSGGKQ YVSSGGSATS
110 120 130 140 150
TTINIGGVQH VSSGGSATSS TINSGGHQHV SSGGSATNTT VNNGGRQTVF
160 170 180 190 200
SGGSAMGTII NSGGDQYVIS GGSATSASVT SGARQFVSSG GIVKATSVNS
210 220 230 240 250
GGRQYVRDGG SATDTVLNNT GRQFVSSGGS AAKTTINSGG GMYLYGGSAT
260 270 280 290 300
GTSIYNGGRQ YVSSGGSATN TTVYSGGRQH VYIDGNVTET TITSGGMLQV
310 320 330 340 350
EAGGSASKVI QNSGGAVITN TSAAVSGTND NGSFSIAGGS AVNMLLENGG
360 370 380 390 400
YLTVFDGHQA SDTMVGSDGT LDVRSGGVLY GTTTLTDKGA LVGDVVTNEG
410 420 430 440 450
NLYYLNNSTA TFTGTLTGTG TLTQEGGNTR FSGLLSQDGG IFLQSGGAMT
460 470 480 490 500
MDALQAKANV TTQSGTTLTL DNGTILTGNV AGDSTGAGDM AVKGASVWHL
510 520 530 540 550
DGDSTVGALT LDNGTVDFRP STTTRMTPAF QAVSLALGSL SGSGTFQMNT
560 570 580 590 600
DIASHTGDML NVAGNASGNF VLDIKNTGLE PVSAGAPLQV VQTGGGDAAF
610 620 630 640 650
TLKGGKVDAG TWEYGLSKEN TNWYLKADTP PPVTPPTNPD ADNPDAGNPD
660 670 680 690 700
AGNPDAGNPD AGNPDAGKPG TGKPDAGTSS SPVRRTTKSV DAVLGMATAP
710 720 730 740 750
AYVFNSELDN LRFRHGDVMQ NTRAPGGVWG RYTGSDNRIS GGASSGYTLT
760 770 780 790 800
QNGFETGADM VFDLSDSSLA VGTFFSYSDN SIKHARGGKS NVDSSGGGLY
810 820 830 840 850
ATWFDNDGYY VDGVLKYNRF NNELRTWMSD GTAVKGDYSQ NGFGGSLEAG
860 870 880 890 900
RTFSLNENAW AQPYVRTTAF RADKKEIRLN NGMKASIGAT KSLQAEAGLK
910 920 930 940 950
LGMTLDVAGK EVKPYLSAAV SHEFSDNNKV RINDTYDFRN DISGTTGKYG
960 970 980
LGVNAQLTPN AGVWAEARYE NGKQTESPIT GGVGFRINF
Length:989
Mass (Da):101,112
Last modified:November 1, 1999 - v1
Checksum:i6A925466416E63BE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109215 Genomic DNA. Translation: AAD41751.1.
FN649414 Genomic DNA. Translation: CBJ01643.1.
RefSeqiWP_001045641.1. NC_017633.1.

Genome annotation databases

EnsemblBacteriaiCBJ01643; CBJ01643; ETEC_2141.
KEGGielh:ETEC_2141.
PATRICi42960174. VBIEscCol136115_2454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF109215 Genomic DNA. Translation: AAD41751.1.
FN649414 Genomic DNA. Translation: CBJ01643.1.
RefSeqiWP_001045641.1. NC_017633.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Q1QX-ray2.11A/B55-350[»]
ProteinModelPortaliQ9XD84.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.B.12.8.3. the autotransporter-1 (at-1) family.

Proteomic databases

PRIDEiQ9XD84.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCBJ01643; CBJ01643; ETEC_2141.
KEGGielh:ETEC_2141.
PATRICi42960174. VBIEscCol136115_2454.

Phylogenomic databases

OMAiNDDARSH.

Enzyme and pathway databases

BioCyciECOL316401:GLD0-2181-MONOMER.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR030930. AIDA.
IPR005546. Autotransporte_beta.
IPR024973. ESPR.
IPR006315. OM_autotransptr_brl.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR004899. Pertactin_central.
IPR003991. Pertactin_virulence_factor.
[Graphical view]
PfamiPF16168. AIDA. 8 hits.
PF03797. Autotransporter. 1 hit.
PF13018. ESPR. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR01484. PRTACTNFAMLY.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 2 hits.
TIGRFAMsiTIGR01414. autotrans_barl. 1 hit.
TIGR04415. O_hepto_targRPT. 7 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIBA_ECOH1
AccessioniPrimary (citable) accession number: Q9XD84
Secondary accession number(s): E3PBM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: November 1, 1999
Last modified: November 11, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

TibA-mediated aggregation is blocked upon capsule expression and fimbriation and is sensitive to pH extremes.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.