Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Vitamin B12-dependent ribonucleotide reductase

Gene

nrdJ

Organism
Streptomyces cattleya
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

adenosylcob(III)alaminBy similarityNote: 5'-deoxyadenosylcobalamine (coenzyme B12).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

Cobalamin, Cobalt, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Vitamin B12-dependent ribonucleotide reductase (EC:1.17.4.1)
Alternative name(s):
Ribonucleoside-diphosphate reductase NrdJ
Gene namesi
Name:nrdJ
OrganismiStreptomyces cattleya
Taxonomic identifieri29303 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 599599Vitamin B12-dependent ribonucleotide reductasePRO_0000231662Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi1003195.SCAT_4578.

Structurei

3D structure databases

ProteinModelPortaliQ9XD73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni193 – 1975Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.

Sequencei

Sequence statusi: Fragment.

Q9XD73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLDNSSCNL ASLNLMKFLR DDTASDGASG AGGNMSLDAE RFAKVVELVI
60 70 80 90 100
TAMDISICFA DFPTEKIGET TRAFRQLGIG YANLGALLMA TGHAYDSDGG
110 120 130 140 150
RALAGAITSL MTGTSYRRSA ELAAVVGPYE GYARNADAHK RVMRQHADAN
160 170 180 190 200
TAAQRMDDLD TPVWAAATEA WQDVLRLGEQ NRFRNAQASV LAPTGTIGLM
210 220 230 240 250
MDCDTTGVEP DLALVKFKKL VGGGSMQIVN NTVPKALKRL GYQPEQVEAV
260 270 280 290 300
VAHIAEHGNV IDAPGLKPEH YEVFDCAMGE RAISPMGHVR MMAAAQPFLS
310 320 330 340 350
GAISKTVNMP ESATVEEIEE IYYEGWKLGL KALAIYRDNC KVGQPLSAKK
360 370 380 390 400
KEEAKTEAPA EVEKVVEYRP VRKRLPKGRP GITTSFTVGG AEGYMTANSY
410 420 430 440 450
PDDGLGEVFL KMSKQGSTLA GMMDAFSIAV SVGLQYGVPL ETYVSKFTNM
460 470 480 490 500
RFEPAGLTDD PDVRMAQSIV DYIFRRLALD FLPFETRSAL GIHSAEERQR
510 520 530 540 550
HLDTGSYEPA EDELDVEGLA QSAPRQAGPA KAATTAPAAK AQEPAAAPSP
560 570 580 590
KQAHNSTELL EIQQGLNADA PLCFSCGTKM RRAGSCYVCE GCGSTSGCS
Length:599
Mass (Da):64,236
Last modified:November 1, 1999 - v1
Checksum:i8679481ADA46B037
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111267 Genomic DNA. Translation: AAD37499.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF111267 Genomic DNA. Translation: AAD37499.1.

3D structure databases

ProteinModelPortaliQ9XD73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1003195.SCAT_4578.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000788. RNR_lg_C.
IPR024434. TSCPD_dom.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF12637. TSCPD. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a regulatory gene from Streptomyces cattleya and study on its cis-acting element."
    Shang G., Wang Y.
    Sci. China, Ser. C, Life Sci. 43:418-424(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiNRDJ_STRCT
AccessioniPrimary (citable) accession number: Q9XD73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.