Reviewed, UniProtKB/Swiss-Prot Q9XD73 (NRDJ_STRCT)

Names and origin

Protein names

Recommended name:
    Vitamin B12-dependent ribonucleotide reductase
    EC=1.17.4.1
Alternative name(s):
    Ribonucleoside-diphosphate reductase nrdJ

Gene names

Name:

nrdJ

Organism

Streptomyces cattleya

Taxonomic identifier

29303 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	599 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reduction of ribonucleotides to deoxyribonucleotides. May function to provide a pool of deoxyribonucleotide precursors for DNA repair during oxygen limitation and/or for immediate growth after restoration of oxygen By similarity.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	5'-deoxyadenosylcobalamine (coenzyme B12) By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase class-2 family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	Cobalamin Cobalt Nucleotide-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside-diphosphate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Vitamin B12-dependent ribonucleotide reductase		PRO_0000231662
Sites
	Active site	8	1	By similarity
	Active site	592	1	By similarity
	Active site	598	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q9XD73-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 8679481ADA46B037	FASTA	599	64,236
	10 20 30 40 50 60 MHLDNSSCNL ASLNLMKFLR DDTASDGASG AGGNMSLDAE RFAKVVELVI TAMDISICFA 70 80 90 100 110 120 DFPTEKIGET TRAFRQLGIG YANLGALLMA TGHAYDSDGG RALAGAITSL MTGTSYRRSA 130 140 150 160 170 180 ELAAVVGPYE GYARNADAHK RVMRQHADAN TAAQRMDDLD TPVWAAATEA WQDVLRLGEQ 190 200 210 220 230 240 NRFRNAQASV LAPTGTIGLM MDCDTTGVEP DLALVKFKKL VGGGSMQIVN NTVPKALKRL 250 260 270 280 290 300 GYQPEQVEAV VAHIAEHGNV IDAPGLKPEH YEVFDCAMGE RAISPMGHVR MMAAAQPFLS 310 320 330 340 350 360 GAISKTVNMP ESATVEEIEE IYYEGWKLGL KALAIYRDNC KVGQPLSAKK KEEAKTEAPA 370 380 390 400 410 420 EVEKVVEYRP VRKRLPKGRP GITTSFTVGG AEGYMTANSY PDDGLGEVFL KMSKQGSTLA 430 440 450 460 470 480 GMMDAFSIAV SVGLQYGVPL ETYVSKFTNM RFEPAGLTDD PDVRMAQSIV DYIFRRLALD 490 500 510 520 530 540 FLPFETRSAL GIHSAEERQR HLDTGSYEPA EDELDVEGLA QSAPRQAGPA KAATTAPAAK 550 560 570 580 590 AQEPAAAPSP KQAHNSTELL EIQQGLNADA PLCFSCGTKM RRAGSCYVCE GCGSTSGCS

References

[1]	"Cloning of a regulatory gene from Streptomyces cattleya." Shang G., Wang Y. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF111267 Genomic DNA. Translation: AAD37499.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.17.4.1. 142837.
Family and domain databases
InterPro	IPR000788. Ribncl_red_lg_C. [Graphical view]
PANTHER	PTHR11573. Ribncl_red_lg_C. 1 hit.
Pfam	PF02867. Ribonuc_red_lgC. 1 hit. [Graphical view]
PRINTS	PR01183. RIBORDTASEM1.
ProtoNet	Search...

Entry information

Entry name

NRDJ_STRCT

Accession

Primary (citable) accession number: Q9XD73

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)