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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107ZincUniRule annotation1
Metal bindingi109ZincUniRule annotation1
Metal bindingi134ZincUniRule annotation1
Metal bindingi136ZincUniRule annotation1
Binding sitei254ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:PA3134
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3134.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001196271 – 494Glutamate--tRNA ligaseAdd BLAST494

Proteomic databases

PaxDbiQ9XCL6.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi208964.PA3134.

Structurei

Secondary structure

1494
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 8Combined sources4
Beta strandi12 – 14Combined sources3
Helixi18 – 33Combined sources16
Beta strandi37 – 40Combined sources4
Helixi52 – 65Combined sources14
Beta strandi70 – 72Combined sources3
Turni73 – 75Combined sources3
Helixi84 – 86Combined sources3
Helixi90 – 100Combined sources11
Beta strandi103 – 107Combined sources5
Helixi139 – 147Combined sources9
Beta strandi153 – 156Combined sources4
Beta strandi161 – 168Combined sources8
Turni169 – 171Combined sources3
Beta strandi172 – 177Combined sources6
Helixi178 – 180Combined sources3
Beta strandi184 – 187Combined sources4
Helixi195 – 205Combined sources11
Beta strandi210 – 214Combined sources5
Helixi215 – 217Combined sources3
Helixi221 – 231Combined sources11
Beta strandi237 – 241Combined sources5
Turni254 – 256Combined sources3
Helixi261 – 266Combined sources6
Helixi271 – 282Combined sources12
Helixi294 – 299Combined sources6
Helixi303 – 305Combined sources3
Helixi315 – 327Combined sources13
Helixi331 – 341Combined sources11
Helixi345 – 356Combined sources12
Helixi362 – 364Combined sources3
Helixi365 – 369Combined sources5
Helixi370 – 372Combined sources3
Beta strandi374 – 376Combined sources3
Helixi381 – 384Combined sources4
Helixi391 – 407Combined sources17
Helixi413 – 426Combined sources14
Helixi431 – 443Combined sources13
Beta strandi444 – 447Combined sources4
Helixi451 – 458Combined sources8
Helixi460 – 474Combined sources15
Helixi479 – 490Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TGTX-ray2.45A/B1-494[»]
ProteinModelPortaliQ9XCL6.
SMRiQ9XCL6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionUniRule annotationAdd BLAST11
Motifi251 – 255"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
InParanoidiQ9XCL6.
KOiK01885.
OMAiQLCYMPL.
PhylomeDBiQ9XCL6.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XCL6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTVRTRIAP SPTGDPHVGT AYIALFNLCF ARQHGGQFIL RIEDTDQLRS
60 70 80 90 100
TRESEQQIYD ALRWLGIEWD EGPDVGGPHG PYRQSERGHI YKKYSDELVE
110 120 130 140 150
KGHAFTCFCT PERLDAVRAE QMARKETPRY DGHCMHLPKD EVQRRLAAGE
160 170 180 190 200
SHVTRMKVPT EGVCVVPDML RGDVEIPWDR MDMQVLMKAD GLPTYFLANV
210 220 230 240 250
VDDHLMGITH VLRGEEWLPS APKLIKLYEY FGWEQPQLCY MPLLRNPDKS
260 270 280 290 300
KLSKRKNPTS ITFYERMGYL PQALLNYLGR MGWSMPDERE KFTLAEMIEH
310 320 330 340 350
FDLSRVSLGG PIFDLEKLSW LNGQWIREQS VEEFAREVQK WALNPEYLMK
360 370 380 390 400
IAPHVQGRVE NFSQIAPLAG FFFSGGVPLD ASLFEHKKLD PTQVRQVLQL
410 420 430 440 450
VLWKLESLRQ WEKERITGCI QAVAEHLQLK LRDVMPLMFP AITGHASSVS
460 470 480 490
VLDAMEILGA DLSRYRLRQA LELLGGASKK ETKEWEKIRD AIPG
Length:494
Mass (Da):56,749
Last modified:December 8, 2000 - v2
Checksum:iEC3EFD97BA5AFD72
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti93K → R in AAD33773 (PubMed:10348873).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139107 Genomic DNA. Translation: AAD33773.1.
AE004091 Genomic DNA. Translation: AAG06522.1.
PIRiE83254.
RefSeqiNP_251824.1. NC_002516.2.
WP_003104596.1. NZ_ASJY01000511.1.

Genome annotation databases

EnsemblBacteriaiAAG06522; AAG06522; PA3134.
GeneIDi882647.
KEGGipae:PA3134.
PATRICi19840849. VBIPseAer58763_3286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF139107 Genomic DNA. Translation: AAD33773.1.
AE004091 Genomic DNA. Translation: AAG06522.1.
PIRiE83254.
RefSeqiNP_251824.1. NC_002516.2.
WP_003104596.1. NZ_ASJY01000511.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5TGTX-ray2.45A/B1-494[»]
ProteinModelPortaliQ9XCL6.
SMRiQ9XCL6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3134.

Proteomic databases

PaxDbiQ9XCL6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06522; AAG06522; PA3134.
GeneIDi882647.
KEGGipae:PA3134.
PATRICi19840849. VBIPseAer58763_3286.

Organism-specific databases

PseudoCAPiPA3134.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252720.
InParanoidiQ9XCL6.
KOiK01885.
OMAiQLCYMPL.
PhylomeDBiQ9XCL6.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022. Glu_tRNA_synth_type1. 1 hit.
InterProiView protein in InterPro
IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
PfamiView protein in Pfam
PF00749. tRNA-synt_1c. 1 hit.
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00178. AA_TRNA_LIGASE_I. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_PSEAE
AccessioniPrimary (citable) accession number: Q9XCL6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: December 8, 2000
Last modified: March 15, 2017
This is version 112 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.