ID   Q9XC48_9ACTN            Unreviewed;        90 AA.
AC   Q9XC48;
DT   01-NOV-1999, integrated into UniProtKB/TrEMBL.
DT   01-NOV-1999, sequence version 1.
DT   27-MAR-2024, entry version 101.
DE   SubName: Full=Peptide synthetase NRPS type II-PCP {ECO:0000313|EMBL:AAD42077.1};
GN   Name=blmI {ECO:0000313|EMBL:AAD42077.1};
OS   Streptomyces verticillus.
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces.
OX   NCBI_TaxID=29309 {ECO:0000313|EMBL:AAD42077.1};
RN   [1] {ECO:0000313|EMBL:AAD42077.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC15003 {ECO:0000313|EMBL:AAD42077.1};
RX   PubMed=10421758; DOI=10.1016/S1074-5521(99)80083-0;
RA   Du L., Shen B.;
RT   "Identification and characterization of a type II peptidyl carrier protein
RT   from the bleomycin producer Streptomyces verticillus ATCC 15003.";
RL   Chem. Biol. 6:507-517(1999).
RN   [2] {ECO:0000313|EMBL:AAD42077.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC15003 {ECO:0000313|EMBL:AAD42077.1};
RX   PubMed=11048953; DOI=10.1016/S1074-5521(00)00011-9;
RA   Du L., Sanchez C., Chen M., Edwards D.J., Shen B.;
RT   "The biosynthetic gene cluster for the antitumor drug bleomycin from
RT   Streptomyces verticillus ATCC15003 supporting functional interactions
RT   between nonribosomal peptide synthetases and a polyketide synthase.";
RL   Chem. Biol. 7:623-642(2000).
RN   [3] {ECO:0000313|EMBL:AAD42077.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=ATCC15003 {ECO:0000313|EMBL:AAD42077.1};
RX   PubMed=10930733; DOI=10.1111/j.1574-6968.2000.tb09225.x;
RA   Du L., Chen M., Sanchez C., Shen B.;
RT   "An oxidation domain in the BlmIII non-ribosomal peptide synthetase
RT   probably catalyzing thiazole formation in the biosynthesis of the anti-
RT   tumor drug bleomycin in Streptomyces verticillus ATCC15003.";
RL   FEMS Microbiol. Lett. 189:171-175(2000).
RN   [4] {ECO:0007829|PDB:4NEO}
RP   X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 2-90.
RX   PubMed=25050442; DOI=10.1002/prot.24485;
RA   Lohman J.R., Ma M., Cuff M.E., Bigelow L., Bearden J., Babnigg G.,
RA   Joachimiak A., Phillips G.N., Shen B.;
RT   "The crystal structure of BlmI as a model for nonribosomal peptide
RT   synthetase peptidyl carrier proteins.";
RL   Proteins 82:1210-1218(2014).
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DR   EMBL; AF210249; AAD42077.1; -; Genomic_DNA.
DR   PDB; 4NEO; X-ray; 2.10 A; A=2-90.
DR   PDBsum; 4NEO; -.
DR   AlphaFoldDB; Q9XC48; -.
DR   SMR; Q9XC48; -.
DR   GO; GO:1904091; F:non-ribosomal peptide synthetase activity; IDA:CACAO.
DR   GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR   GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProt.
DR   GO; GO:1901362; P:organic cyclic compound biosynthetic process; IEA:UniProt.
DR   Gene3D; 1.10.1200.10; ACP-like; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR020806; PKS_PP-bd.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   InterPro; IPR006162; Ppantetheine_attach_site.
DR   PANTHER; PTHR45527:SF1; FATTY ACID SYNTHASE; 1.
DR   PANTHER; PTHR45527; NONRIBOSOMAL PEPTIDE SYNTHETASE; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   SMART; SM00823; PKS_PP; 1.
DR   SUPFAM; SSF47336; ACP-like; 1.
DR   PROSITE; PS50075; CARRIER; 1.
DR   PROSITE; PS00012; PHOSPHOPANTETHEINE; 1.
PE   1: Evidence at protein level;
KW   3D-structure {ECO:0007829|PDB:4NEO};
KW   Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553}.
FT   DOMAIN          9..84
FT                   /note="Carrier"
FT                   /evidence="ECO:0000259|PROSITE:PS50075"
SQ   SEQUENCE   90 AA;  9957 MW;  D28FDB4C4D2D4C8C CRC64;
     MSAPRGERTR RRALERDIAA IWAETLGRDS VGPHEDFAAL GGNSIHAIKI TNRVEELVDA
     ELSIRVLLET RTVAGMTDHV HATLTGERDR
//