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Protein

Ribonucleoside-diphosphate reductase subunit beta

Gene

nrdF

Organism
Mycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity).By similarity

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Cofactori

Fe cationBy similarityNote: Binds 2 iron ions per subunit.By similarity

Pathway: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Iron 1By similarity
Metal bindingi121 – 1211Iron 1By similarity
Active sitei125 – 1251By similarity
Metal bindingi215 – 2151Iron 2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMGAL710127:GC09-45-MONOMER.
UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit beta (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase small subunit
Gene namesi
Name:nrdF
Ordered Locus Names:MYCGA0450
ORF Names:MGA_0698
OrganismiMycoplasma gallisepticum (strain R(low / passage 15 / clone 2))
Taxonomic identifieri710127 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000001418 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 339339Ribonucleoside-diphosphate reductase subunit betaPRO_0000190482Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9XC20.
SMRiQ9XC20. Positions 22-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000246153.
KOiK00526.
OMAiYYQADEP.
OrthoDBiEOG68DD19.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9XC20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNNNKYYDK SFSPLGYVAN GQKGVMRSIN WNVINDPKDL EVWTRVTQNF
60 70 80 90 100
WLPEKIPVSN DLKSWNELTP EWKQLVTRTF TGLTLLDTIQ CTLGDIAQIP
110 120 130 140 150
NSLTDHEQFV YANFSFMVGV HARSYGTIFS TLNTSDEIEE AHEWVINNEK
160 170 180 190 200
LQARAKFLVP YYTSDDPLKS KIAAALMPGF LLYGGFYLPF YLAARGKLPN
210 220 230 240 250
TSDIIRLILR DKVIHNYYSG YKYRLKVQKL PKEKQEEYKK FVFEILYKLI
260 270 280 290 300
ELEKDFLREL YDGFGLADEA IAFSLYNAGK FLQNCGYESP FTPEETKISP
310 320 330
EVFAQLSARA DENHDFFSGN GSSYIMGVTE ETQDDDWEF
Length:339
Mass (Da):39,168
Last modified:November 1, 1999 - v1
Checksum:iA8711B1B51E4F47F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152114 Genomic DNA. Translation: AAD45275.1.
AE015450 Genomic DNA. Translation: AAP56395.1.
RefSeqiNP_852827.1. NC_004829.2.
WP_011113274.1. NC_004829.2.

Genome annotation databases

EnsemblBacteriaiAAP56395; AAP56395; MGA_0698.
GeneIDi1090094.
KEGGimga:MGA_0698.
PATRICi20007760. VBIMycGal115357_0048.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF152114 Genomic DNA. Translation: AAD45275.1.
AE015450 Genomic DNA. Translation: AAP56395.1.
RefSeqiNP_852827.1. NC_004829.2.
WP_011113274.1. NC_004829.2.

3D structure databases

ProteinModelPortaliQ9XC20.
SMRiQ9XC20. Positions 22-307.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAP56395; AAP56395; MGA_0698.
GeneIDi1090094.
KEGGimga:MGA_0698.
PATRICi20007760. VBIMycGal115357_0048.

Phylogenomic databases

eggNOGiCOG0208.
HOGENOMiHOG000246153.
KOiK00526.
OMAiYYQADEP.
OrthoDBiEOG68DD19.

Enzyme and pathway databases

UniPathwayiUPA00326.
BioCyciMGAL710127:GC09-45-MONOMER.

Family and domain databases

Gene3Di1.10.620.20. 1 hit.
InterProiIPR009078. Ferritin-like_SF.
IPR012348. RNR-rel.
IPR026494. RNR_NrdF.
IPR000358. RNR_small.
[Graphical view]
PANTHERiPTHR23409. PTHR23409. 1 hit.
PfamiPF00268. Ribonuc_red_sm. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
TIGRFAMsiTIGR04171. RNR_1b_NrdF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Skamrov A.V., Gol'dman M.A., Feoktistova E.S., Bibilashvili R.S.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: A5969Var.B.
  2. "The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
    Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
    Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: R(low / passage 15 / clone 2).

Entry informationi

Entry nameiRIR2_MYCGA
AccessioniPrimary (citable) accession number: Q9XC20
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: November 1, 1999
Last modified: June 24, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Caution

Seems to lack two of the iron-binding residues.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.