Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9XC19 (TYSY_MYCGA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:MYCGA0460
ORF Names:MGA_0699
OrganismMycoplasma gallisepticum (strain R(low / passage 15 / clone 2)) [Complete proteome] [HAMAP]
Taxonomic identifier710127 [NCBI]
Taxonomic lineageBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000140982

Sites

Active site1701 By similarity

Experimental info

Sequence conflict691I → M in AAD45276. Ref.1
Sequence conflict2181G → W in AAD45276. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9XC19 [UniParc].

Last modified August 15, 2003. Version 2.
Checksum: 57FB51D2CE1635B7

FASTA28933,839
        10         20         30         40         50         60 
MKTYLDLLEK VMTKGEVKKD RTNTGTISYF GTQSRYDLRE GFPLLTTKKM AWKAICHELL 

        70         80         90        100        110        120 
WFLKGDTNIK YLVDHNVNIW NEWPYEAFKK SNDFKGESLQ DFVEKIKTDN QFAQQYGKLG 

       130        140        150        160        170        180 
PVYGKQWRDF NGVDQIEWVI NEIKTNPFSR RLIVSAWNPG EIKEMALPPC HSFFQFFVNE 

       190        200        210        220        230        240 
KYEISLQLYQ RSGDLFLGVP FNIASYSLLL LMVAQVTGLK PAEFIHTIGD THIYSNHLEQ 

       250        260        270        280 
VKIQLQREPK KLPKVTLNPD IKNIFDFRFE DISLENYEHH DPIKGKVAV 

« Hide

References

« Hide 'large scale' references
[1]Skamrov A.V., Gol'dman M.A., Feoktistova E.S., Bibilashvili R.S.
Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: A5969Var.B.
[2]"The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain R(low)."
Papazisi L., Gorton T.S., Kutish G., Markham P.F., Browning G.F., Nguyen D.K., Swartzell S., Madan A., Mahairas G., Geary S.J.
Microbiology 149:2307-2316(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: R(low / passage 15 / clone 2).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF152114 Genomic DNA. Translation: AAD45276.1.
AE015450 Genomic DNA. Translation: AAP56396.1.
RefSeqNP_852828.1. NC_004829.2.

3D structure databases

ProteinModelPortalQ9XC19.
SMRQ9XC19. Positions 2-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233150.MGA_0699.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAP56396; AAP56396; MGA_0699.
GeneID1090095.
KEGGmga:MGA_0699.
PATRIC20007762. VBIMycGal115357_0049.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMANLGPVYG.
OrthoDBEOG6K6V53.
ProtClustDBPRK01827.

Enzyme and pathway databases

BioCycMGAL710127:GC09-46-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_MYCGA
AccessionPrimary (citable) accession number: Q9XC19
Entry history
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: August 15, 2003
Last modified: February 19, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways