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Q9XC07

- GLND_VIBF1

UniProt

Q9XC07 - GLND_VIBF1

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Vibrio fischeri (strain ATCC 700601 / ES114)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciAFIS312309:GIWP-2066-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:VF_1964
OrganismiVibrio fischeri (strain ATCC 700601 / ES114)
Taxonomic identifieri312309 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeAliivibrio
ProteomesiUP000000537: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 873873Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192772Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi312309.VF_1964.

Structurei

3D structure databases

ProteinModelPortaliQ9XC07.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini451 – 584134HDUniRule annotationAdd
BLAST
Domaini693 – 77785ACT 1UniRule annotationAdd
BLAST
Domaini800 – 87374ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 332332UridylyltransferaseAdd
BLAST
Regioni333 – 692360Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XC07-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKYLSPLSLS DTQLNITELK QQLTLFSQYQ INAFHQHKAV SDLVLERSHY
60 70 80 90 100
FDQLLSRLWQ FFKFDDIANT SLIAVGGYGR SELHPLSDID ILILTENNTN
110 120 130 140 150
DAFCQKVGEL VTLLWDLKLE VGHSVRSIAE CIEIGQNDLT VATNLQEARY
160 170 180 190 200
ISGNKELSHQ LKLKIHSDSF WPSELFYQAK IDEQKKRHSR YHDTTYNLEP
210 220 230 240 250
DIKSSPGGLR DIHTLSWIAR RHFGATSLLE MSQAGFLTDA EYRELLECQE
260 270 280 290 300
FLWRVRFALH IELKRYDNRL TFGHQASVAE HLGFIGEGNR GVERMMKEFY
310 320 330 340 350
RTLRRVAELN SMLLKIFDQA ILHQGEQDDA IIIDDDFQRR GRLIEARKPA
360 370 380 390 400
LFQARPDTIL DMFLLMANDS TIDGVAPPTM RQLRTARRRL NRFLCEIPEA
410 420 430 440 450
KEKFLQLTQH PNALNNAFSS MHKLGVLSAY LPQWSHIVGQ MQFDLFHAYT
460 470 480 490 500
VDEHSIRLLK HINKFSDTTN RDKHPICCEI FPKIMKKELL IIAAIFHDIA
510 520 530 540 550
KGRGGDHSEL GAVDAYDFCI SHGLSKPEAN LVSWLVKSHL LMSVTAQRRD
560 570 580 590 600
IYDPDVITEF AKQVRDEERL DYLVCLTVAD ICATNPDLWN SWKRSLIADL
610 620 630 640 650
YNATQRALRR GLENPPDLRD RIRHNQQMAS AQLRSEGFTQ WEVDALWRRF
660 670 680 690 700
KADYFLRHTH KQIAWHASHL LRHQDKEKSL ILISKNASRG GTEIFVYSKD
710 720 730 740 750
QPHLFATVAA ELDRRSITIY DAQVMSSKDG YALDTFMVLD QNDDPIDEER
760 770 780 790 800
QQRLIDQLYD VKLNDQATHI KTRRPPRQLQ HFNVKTRMEF LPTKTGKRTL
810 820 830 840 850
MEFVALDTPG LLATVGATFA QLGINLHAAK ITTIGERAED LFILTSDVGG
860 870
RLDDEKQAEL ELALVKNVAR LSS
Length:873
Mass (Da):100,599
Last modified:April 26, 2005 - v2
Checksum:i99CB3E00D0B5C568
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF152563 Genomic DNA. Translation: AAD38384.1.
CP000020 Genomic DNA. Translation: AAW86459.1.
RefSeqiYP_205347.1. NC_006840.2.

Genome annotation databases

EnsemblBacteriaiAAW86459; AAW86459; VF_1964.
GeneIDi3279229.
KEGGivfi:VF_1964.
PATRICi20114620. VBIVibFis127983_1991.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF152563 Genomic DNA. Translation: AAD38384.1 .
CP000020 Genomic DNA. Translation: AAW86459.1 .
RefSeqi YP_205347.1. NC_006840.2.

3D structure databases

ProteinModelPortali Q9XC07.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 312309.VF_1964.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAW86459 ; AAW86459 ; VF_1964 .
GeneIDi 3279229.
KEGGi vfi:VF_1964.
PATRICi 20114620. VBIVibFis127983_1991.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci AFIS312309:GIWP-2066-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Unexpected effects of a transposon insertion in the Vibrio fischeri glnD gene: defects in iron uptake and symbiotic persistence in addition to nitrogen utilization."
    Graf J., Ruby E.G.
    Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700601 / ES114.

Entry informationi

Entry nameiGLND_VIBF1
AccessioniPrimary (citable) accession number: Q9XC07
Secondary accession number(s): Q5E3D7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: April 26, 2005
Last modified: October 29, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3