Reviewed,
UniProtKB/Swiss-Prot Q9XBQ9 (STHA_AZOVI)
Last modified
February 9, 2010.
Version 66.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Soluble pyridine nucleotide transhydrogenase Short name=STH EC=1.6.1.1 Alternative name(s): NAD(P)(+) transhydrogenase [B-specific] | ||||
| Gene names |
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| Organism | Azotobacter vinelandii | ||||
| Taxonomic identifier | 354 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter |
Protein attributes
| Sequence length | 464 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation. HAMAP MF_00247 |
| Catalytic activity | NADPH + NAD+ = NADP+ + NADH. HAMAP MF_00247 |
| Cofactor | Binds 1 FAD per subunit. HAMAP MF_00247 |
| Subunit structure | Homooctamer. Forms a polymeric filamentous structure in purified form. Ref.3 |
| Subcellular location | |
| Sequence similarities | Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | FAD Flavoprotein NAD NADP |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | NADP metabolic process Inferred from electronic annotation. Source: HAMAP cell redox homeostasisInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | FAD binding Inferred from electronic annotation. Source: InterPro NAD(P)+ transhydrogenase (B-specific) activityInferred from electronic annotation. Source: HAMAP |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 464 | 464 | Soluble pyridine nucleotide transhydrogenase HAMAP MF_00247 | PRO_0000068060 | |||||
Regions | |||||||||
| Nucleotide binding | 35 – 44 | 10 | FAD By similarity | ||||||
Sequences
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References
| [1] | "Cloning of the sth gene from Azotobacter vinelandii and construction of chimeric soluble pyridine nucleotide transhydrogenases." Boonstra B., Bjorklund L., French C.E., Wainwright I., Bruce N.C. FEMS Microbiol. Lett. 191:87-93(2000) [PubMed: 11004404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617. |
| [2] | "Pyridine nucleotide transhydrogenase from Azotobacter vinelandii. Improved purification, physical properties and subunit arrangement in purified polymers." Voordouw G., van der Vies S.M., Eweg J.K., Veeger C., van Breemen J.F.L., van Bruggen E.F.J. Eur. J. Biochem. 111:347-355(1980) [PubMed: 7460901] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617. |
| [3] | "Structure of pyridine nucleotide transhydrogenase from Azotobacter vinelandii." Voordouw G., Veeger C., van Breemen J.F.L., van Bruggen E.F.J. Eur. J. Biochem. 98:447-454(1979) [PubMed: 39756] [Abstract] Cited for: SUBUNIT. Strain: ATCC 478 / NRS 16 / DSM 2289 / VKM B-1617. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF159108 Genomic DNA. Translation: AAD40691.1. |
3D structure databases | |
| ModBase | Search... |
Enzyme and pathway databases | |
| BRENDA | 1.6.1.1. 883. |
Family and domain databases | |
| HAMAP | MF_00247. SthA. [Tree] |
| InterPro | IPR016156. FAD/NAD-linked_Rdtase_dimer. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR000815. Hg_reductase. IPR004099. Pyr_nucl-diS_OxRdtase_dimer. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view] |
| Gene3D | G3DSA:3.30.390.30. Pyr_redox_dim. 1 hit. |
| Pfam | PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. PF02852. Pyr_redox_dim. 1 hit. [Graphical view] |
| PRINTS | PR00368. FADPNR. PR00945. HGRDTASE. |
| ProtoNet | Search... |
Entry information
| Entry name | STHA_AZOVI | ||||||||
| Accession | Primary (citable) accession number: Q9XBQ9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||

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