Reviewed, UniProtKB/Swiss-Prot Q9XBM7 (PTM3C_KLEPN)

Names and origin

Protein names

Recommended name:
    PTS system mannitol-specific EIICBA component
Alternative name(s):
    EIICBA-Mtl
      Short name=EII-Mtl
Including the following 3 domains:
    1- Recommended name:
            Mannitol permease IIC component
        Alternative name(s):
            PTS system mannitol-specific EIIC component
    2- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system mannitol-specific EIIB component
    3- Recommended name:
            Mannitol-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system mannitol-specific EIIA component

Gene names

Name:

mtlA

Organism

Klebsiella pneumoniae

Taxonomic identifier

573 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	635 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine. Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subunit structure	Homodimer By similarity.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Post-translational modification	An intramolecular phosphotransfer takes places between His-554 and Cys-384.
Sequence similarities	Contains 1 PTS EIIA type-2 domain. Contains 1 PTS EIIB type-2 domain. Contains 1 PTS EIIC type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 635	635	PTS system mannitol-specific EIICBA component		PRO_0000186615
Regions
	Transmembrane	24 – 44	21	Potential
	Transmembrane	48 – 68	21	Potential
	Transmembrane	78 – 98	21	Potential
	Transmembrane	137 – 157	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	213 – 233	21	Potential
	Transmembrane	245 – 265	21	Potential
	Transmembrane	269 – 289	21	Potential
	Transmembrane	313 – 333	21	Potential
	Domain	12 – 342	331	PTS EIIC type-2
	Domain	378 – 473	96	PTS EIIB type-2
	Domain	494 – 635	142	PTS EIIA type-2
Sites
	Active site	384	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	554	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9XBM7-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9C76B771A03815E7	FASTA	635	67,698
	10 20 30 40 50 60 MSSDIKIKVQ SFGRFLSNMV MPNIGAFIAW GIITALFIPT GWLPNETLAK LVGPMITYLL 70 80 90 100 110 120 PLLIGYTGGK LVGGERGGVV GAITTMGVIV GADMPMFLGS MIAGPLGGYC IKKFDNWVDG 130 140 150 160 170 180 KIKSGFEMLV NNFSAGIIGM ILAILAFLGI GPAVEVLSKI LAAGVNFMVA HDMLPLASIF 190 200 210 220 230 240 VEPAKILFLN NAINHGIFSP LGIQQSHELG KSIFFLIEAN PGPGMGVLLA YMFFGRGSAK 250 260 270 280 290 300 QSAGGAAIIH FLGGIHEIYF PYVLMNPRLI LAVILGGMTG VFTLTILNGG LVSPASPGSI 310 320 330 340 350 360 LAVLAMTPKG AYFANIAAII AAMAVSFVVS AVLFKTSKVK ERSDIEAATR RMHDMKAESK 370 380 390 400 410 420 GASPLAAGNV TNDLSHVRKI IVACDAGMGS SAMGAGVLRK KVQDAGLSNI SVTNSAINNL 430 440 450 460 470 480 PPDVDLVITH RDLTERAMRQ VPQAQHISLT NFLDSGLYTS LTERLVAAQR HIDNEVKVTD 490 500 510 520 530 540 SLKDSFDDTN NNLFQLGADN IFLGRKAATK EEANSFAGEQ LVKGGYVEPE YVQAMLDREK 550 560 570 580 590 600 LTSTYLGESI AVPHGTIEAK DRVLKTGVVF CQYPEGVRFG EEEDEVARLV IGIAARNNEH 610 620 630 IQVITSLTNA LDDETVIERL AKTTSVDEVL ALLNK

References

[1]	"The mtl genes and the mannitol-1-phosphate dehydrogenase from Klebsiella pneumoniae KAY2026." Otte S., Lengeler J.W. FEMS Microbiol. Lett. 194:221-227(2001) [PubMed: 11164312] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1033-5P14 / KAY2026.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF166095 Genomic DNA. Translation: AAD45385.1.
3D structure databases
SMR	Q9XBM7. Positions 375-471, 492-633.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.69. 758.
Family and domain databases
InterPro	IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR013011. PTS_EIIB_2. IPR003501. PTS_EIIB_2/3. IPR003352. PTS_EIIC. IPR013014. PTS_EIIC_2. IPR004718. PTS_IIC_mtl. [Graphical view]
Gene3D	G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit.
Pfam	PF00359. PTS_EIIA_2. 1 hit. PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00851. mtlA. 1 hit.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51099. PTS_EIIB_TYPE_2. 1 hit. PS51104. PTS_EIIC_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTM3C_KLEPN

Accession

Primary (citable) accession number: Q9XBM7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)