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Protein

(5R)-carbapenem-3-carboxylate synthase

Gene

carC

Organism
Pectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the Fe2+ and alpha-ketoglutarate-dependent conversion of (3S,5S)-carbapenam to (5R)-carbapenem, an essential step in carbapenem antibiotic biosynthesis.4 Publications

Catalytic activityi

(3S,5S)-carbapenam-3-carboxylate + 2-oxoglutarate + O2 = (5R)-carbapen-2-em-3-carboxylate + succinate + CO2 + H2O.3 Publications

Cofactori

Fe2+2 PublicationsNote: Binds 1 Fe2+ per subunit.2 Publications

Enzyme regulationi

Inhibited by L-N-acetylproline and by D-N-acetylproline.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi101 – 1011Iron; catalytic1 Publication
Metal bindingi103 – 1031Iron; catalytic1 Publication
Binding sitei104 – 1041Substrate; via amide nitrogenSequence analysis
Binding sitei130 – 1301Alpha-ketoglutarate
Metal bindingi251 – 2511Iron; catalytic1 Publication
Binding sitei253 – 2531Alpha-ketoglutarate
Binding sitei263 – 2631Alpha-ketoglutarate
Binding sitei267 – 2671Alpha-ketoglutarate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13573.
BRENDAi1.14.20.3. 2140.

Names & Taxonomyi

Protein namesi
Recommended name:
(5R)-carbapenem-3-carboxylate synthase (EC:1.14.20.3)
Alternative name(s):
Carbapenem synthase
Gene namesi
Name:carC
OrganismiPectobacterium carotovorum subsp. carotovorum (Erwinia carotovora subsp. carotovora)
Taxonomic identifieri555 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePectobacterium

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 273273(5R)-carbapenem-3-carboxylate synthasePRO_0000424199Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of trimers.1 Publication

Structurei

Secondary structure

1
273
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 194Combined sources
Helixi21 – 266Combined sources
Helixi29 – 3911Combined sources
Beta strandi41 – 444Combined sources
Helixi51 – 599Combined sources
Beta strandi62 – 643Combined sources
Helixi69 – 713Combined sources
Beta strandi81 – 844Combined sources
Beta strandi94 – 963Combined sources
Beta strandi98 – 1014Combined sources
Helixi107 – 1093Combined sources
Beta strandi112 – 12312Combined sources
Beta strandi125 – 1284Combined sources
Beta strandi130 – 1334Combined sources
Helixi135 – 1417Combined sources
Helixi144 – 1529Combined sources
Beta strandi155 – 1595Combined sources
Helixi162 – 1654Combined sources
Turni166 – 1683Combined sources
Beta strandi178 – 1814Combined sources
Beta strandi186 – 1883Combined sources
Beta strandi201 – 2066Combined sources
Helixi211 – 22515Combined sources
Turni228 – 2303Combined sources
Beta strandi231 – 2344Combined sources
Beta strandi241 – 2455Combined sources
Turni246 – 2483Combined sources
Beta strandi250 – 2534Combined sources
Beta strandi262 – 27110Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NX4X-ray2.40A/B/C1-273[»]
1NX8X-ray2.30A/B/C1-273[»]
4OJ8X-ray2.10A/B/C1-273[»]
ProteinModelPortaliQ9XB59.
SMRiQ9XB59. Positions 2-272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9XB59.

Family & Domainsi

Sequence similaritiesi

Belongs to the TfdA dioxygenase family.Curated

Family and domain databases

InterProiIPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF02668. TauD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9XB59-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEIVKFNPV MASGFGAYID HRDFLEAKTE TIKNLLMRQG FVVVKNLDID
60 70 80 90 100
SDTFRDIYSA YGTIVEYADE KIGVGFGYRD TLKLEGEKGK IVTGRGQLPF
110 120 130 140 150
HADGGLLLSQ VDQVFLYAAE IKNVKFRGAT TVCDHALACQ EMPAHLLRVL
160 170 180 190 200
EEETFEVRVL ERGYYVDVSP DGWFKVPVFT DLGWVRKMLI YFPFDEGQPA
210 220 230 240 250
SWEPRIVGFT DHETQAFFQE LGAFLKQPRY YYKHFWEDGD LLIMDNRRVI
260 270
HEREEFNDDD IVRRLYRGQT ADI
Length:273
Mass (Da):31,611
Last modified:November 1, 1999 - v1
Checksum:i86F1AED4B09E84E4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17224 Genomic DNA. Translation: AAD38231.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17224 Genomic DNA. Translation: AAD38231.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NX4X-ray2.40A/B/C1-273[»]
1NX8X-ray2.30A/B/C1-273[»]
4OJ8X-ray2.10A/B/C1-273[»]
ProteinModelPortaliQ9XB59.
SMRiQ9XB59. Positions 2-272.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13573.
BRENDAi1.14.20.3. 2140.

Miscellaneous databases

EvolutionaryTraceiQ9XB59.

Family and domain databases

InterProiIPR003819. TauD/TfdA-like.
[Graphical view]
PfamiPF02668. TauD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of the carbapenem gene cluster of Erwinia carotovora: definition of the antibiotic biosynthetic genes and evidence for a novel beta-lactam resistance mechanism."
    McGowan S.J., Sebaihia M., O'Leary S., Hardie K.R., Williams P., Stewart G.S., Bycroft B.W., Salmond G.P.
    Mol. Microbiol. 26:545-556(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: ATCC 39048 / GS101.
  2. "Carbapenem biosynthesis: confirmation of stereochemical assignments and the role of CarC in the ring stereoinversion process from L-proline."
    Stapon A., Li R., Townsend C.A.
    J. Am. Chem. Soc. 125:8486-8493(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY.
  3. "Biosynthesis of carbapenem antibiotics: new carbapenam substrates for carbapenem synthase (CarC)."
    Sleeman M.C., Smith P., Kellam B., Chhabra S.R., Bycroft B.W., Schofield C.J.
    ChemBioChem 5:879-882(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, COFACTOR, CATALYTIC ACTIVITY.
  4. Cited for: X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) IN COMPLEX WITH IRON; N-ACETYLPROLINE AND 2-OXOGLUTARATE, CATALYTIC ACTIVITY, FUNCTION, SUBUNIT, ENZYME REGULATION, COFACTOR.

Entry informationi

Entry nameiCARC_PECCC
AccessioniPrimary (citable) accession number: Q9XB59
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: November 1, 1999
Last modified: November 11, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.