Q9XAQ9 (NUOF_STRCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-quinone oxidoreductase subunit F
EC=1.6.99.5

Alternative name(s):
NADH dehydrogenase I subunit F
NDH-1 subunit F

Gene names

Name:	nuoF
Ordered Locus Names:	SCO4567
ORF Names:	SCD16A.16c

Organism

Streptomyces coelicolor

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD+ + quinol.
Cofactor	Binds 1 FMN Potential. Binds 1 4Fe-4S cluster Potential.
Sequence similarities	Belongs to the complex I 51 kDa subunit family.

Ontologies

Keywords
Ligand	4Fe-4S FMN Flavoprotein Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	NADH-quinone oxidoreductase subunit F		PRO_0000118581
Regions
	Nucleotide binding	67 – 76	10	NAD By similarity
	Nucleotide binding	179 – 226	48	FMN By similarity
Sites
	Metal binding	355	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	358	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	361	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	401	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9XAQ9 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 9D0CDE00E89988E5	FASTA	449	48,849
	10 20 30 40 50 60 MMTVAAEIRG SNPEKLLAPV LSAFWDEDES WTLDVYRRHE GYEGLRKALA MAPDDLIAYV 70 80 90 100 110 120 KESGLRGRGG AGFPTGMKWQ FIPQGDGKPH YLVVNADESE PGTCKDIPLL FANPHSLIEG 130 140 150 160 170 180 IVIACYAIRS SHAFIYLRGE VVPVLRRLHE AVREAYAAGF LGENILGSGL DLTLTVHAGA 190 200 210 220 230 240 GAYICGEETA LLDSLEGRRG QPRLRPPFPA VAGLYACPTV VNNVESIASV PAILNKGKDW 250 260 270 280 290 300 FRSMGSEKSP GFTLYSLSGH VAGPGQYEAP LGITLRQLLD MSGGMRPGHR LKFWTPGGSS 310 320 330 340 350 360 TPMFTDEHLD VPLDYEGVGA AGSMLGTKAL QCFDETTCVV RAVTRWTEFY AHESCGKCTP 370 380 390 400 410 420 CREGTYWLVQ LLRDIEAGKG QMSDLDKLND IADNINGKSF CALGDGAASP IFSSLKYFRE 430 440 EYEEHITGRG CPFDPAKSTA WADRTEVKA

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939120 Genomic DNA. Translation: CAB44526.1.
PIR	T34619.
RefSeq	NP_628729.1. NC_003888.3.
3D structure databases
ProteinModelPortal	Q9XAQ9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1100007.
GenomeReviews	Gene locus SCO4567 in contig AL645882_GR.
KEGG	sco:SCO4567.
NMPDR	fig|100226.1.peg.4522.
PATRIC	23738950. VBIStrCoe124346_4639.
Phylogenomic databases
HOGENOM	HBG669338.
OMA	DTYRRHD.
PhylomeDB	Q9XAQ9.
ProtClustDB	CLSK2755161.
Family and domain databases
InterPro	IPR001949. NADH-UbQ_OxRdtase_51kDa_CS. IPR019575. NADH-UbQ_OxRdtase_Fsu_4Fe4S-bd. IPR011537. NADH-UbQ_OxRdtase_suF. IPR011538. NADH_UbQ_OxRdtase_51kDa_su. IPR019554. Soluble_ligand-bd. [Graphical view]
KO	K00335.
Pfam	PF01512. Complex1_51K. 1 hit. PF10589. NADH_4Fe-4S. 1 hit. PF10531. SLBB. 1 hit. [Graphical view]
SMART	SM00928. NADH_4Fe-4S. 1 hit. [Graphical view]
TIGRFAMs	TIGR01959. NuoF_fam. 1 hit.
PROSITE	PS00644. COMPLEX1_51K_1. 1 hit. PS00645. COMPLEX1_51K_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NUOF_STRCO

Accession

Primary (citable) accession number: Q9XAQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program