Reviewed, UniProtKB/Swiss-Prot Q9X9W0 (DAPA2_STRCO)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase 2
      Short name=DHDPS 2
    EC=4.2.1.52

Gene names

Name:	dapA2
Ordered Locus Names:	SCO1912
ORF Names:	SCI7.30

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	305 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 305	305	Dihydrodipicolinate synthase 2 HAMAP MF_00418		PRO_0000103166
Regions
	Region	56 – 57	2	Pyruvate binding By similarity
Sites
	Active site	169	1	Schiff-base intermediate with substrate By similarity
	Binding site	114	1	Pyruvate By similarity
	Site	141	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X9W0-1 [UniParc]. Last modified October 19, 2002. Version 2. Checksum: 978C23AE67B00582	FASTA	305	31,261
	10 20 30 40 50 60 MTTDDDSAPF GRSLCAMVTP FTGSGALDLD GAQRLADRLV ARGCDGLVLS GTTGESPTTT 70 80 90 100 110 120 DAEKAALVTA VREAVGDRAA LVAGVGTADT RHTVELALAA EKAGADGLLV VAPYYSRPPQ 130 140 150 160 170 180 DALEAHFREV ADASGLPVML YDIPGRTGTR IEPDTVVRLA AHPRIVAVKD CAYDLLGTQK 190 200 210 220 230 240 VLSRTGLAYY AGCDEQILPL YAIGAAGYVS TVANVVPELF RAVLDAFDAG DTGRAALLQR 250 260 270 280 290 300 RAVPLVESMM AAGLPGTVTA KALLGALGLP AGPVRAPLRS ADRETTAGLL AAYGELAADA GQSQA

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL939110 Genomic DNA. Translation: CAB46413.1. Different initiation.
PIR	T36926.
RefSeq	NP_626178.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHP based on UniProtKB P05640.
ModBase	Search...
Genome annotation databases
GeneID	1097346.
GenomeReviews	Gene locus SCO1912 in contig AL645882_GR.
KEGG	sco:SCO1912.
NMPDR	fig|100226.1.peg.1879.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9X9W0.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO1912-MON.
BRENDA	4.2.1.52. 1084.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA2_STRCO

Accession

Primary (citable) accession number: Q9X9W0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 19, 2002
Last modified:	September 22, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)