Reviewed, UniProtKB/Swiss-Prot Q9X913 (HPPA_STRCO)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	SCO3547
ORF Names:	SCH5.10c

Organism

Streptomyces coelicolor [Complete proteome] [HAMAP]

Taxonomic identifier

1902 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity. HAMAP MF_01130
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity. HAMAP MF_01130
Subunit structure	Homodimer Potential. HAMAP MF_01130
Subcellular location	Cell membrane; Multi-pass membrane protein Probable HAMAP MF_01130.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217032
Regions
	Transmembrane	20 – 39	20	Potential
	Transmembrane	74 – 91	18	Potential
	Transmembrane	101 – 123	23	Potential
	Transmembrane	164 – 186	23	Potential
	Transmembrane	191 – 213	23	Potential
	Transmembrane	260 – 279	20	Potential
	Transmembrane	284 – 306	23	Potential
	Transmembrane	319 – 341	23	Potential
	Transmembrane	365 – 387	23	Potential
	Transmembrane	408 – 430	23	Potential
	Transmembrane	445 – 467	23	Potential
	Transmembrane	512 – 534	23	Potential
	Transmembrane	563 – 585	23	Potential
	Transmembrane	629 – 651	23	Potential
	Transmembrane	655 – 677	23	Potential
	Transmembrane	715 – 737	23	Potential
	Transmembrane	747 – 766	20	Potential
Sites
	Site	507	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X913-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 1525557A88198CA8	FASTA	794	80,650
	10 20 30 40 50 60 MAELPTSHLA AAVLTDGNRA LVAVIAVVAL AALVLAGVLV RQVLAAGEGT DSMKKIAAAV 70 80 90 100 110 120 QEGANAYLAR QLRTLGVFAV VVFFLLMLLP ADDWNQRAGR SIFFLIGAAF SAATGYIGMW 130 140 150 160 170 180 LAVRSNVRVA AAAREATPAP GEPEKDLALV SHKATKIAFR TGGVVGMFTV GLGLLGACCV 190 200 210 220 230 240 VLVYAADAPK VLEGFGLGAA LIAMFMRVGG GIFTKAADVG ADLVGKVEQG IPEDDPRNAA 250 260 270 280 290 300 TIADNVGDNV GDCAGMAADL FESYAVTLVA ALILGKVAFG DFGLAFPLLV PAIGVLTAMI 310 320 330 340 350 360 GIFAVAPRRS DRSGMSAINR GFFISAVISL VLVAVAVFVY LPGKYADLDG VTDAAIAGKS 370 380 390 400 410 420 GDPRILALVA VAIGIVLAAL IQQLTGYFTE TTRRPVKDIG KSSLTGPATV VLAGISLGLE 430 440 450 460 470 480 SAVYTALLIG LGVYGAFLLG GTSIMLALFA VALAGTGLLT TVGVIVAMDT FGPVSDNAQG 490 500 510 520 530 540 IAEMSGDVEG AGAQVLTDLD AVGNTTKAIT KGIAIATAVL AAAALFGSYR DAITTGAADV 550 560 570 580 590 600 GEKLSGEGAP MTLMMDISQP NNLVGLIAGA AVVFLFSGLA INAVSRSAGA VVYEVRRQFR 610 620 630 640 650 660 ERPGIMDYSE KPEYGKVVDI CTRDALRELA TPGLLAVMAP IFIGFTLGVG ALGAFLAGAI 670 680 690 700 710 720 GAGTLMAVFL ANSGGSWDNA KKLVEDGHHG GKGSEAHAAT VIGDTVGDPF KDTAGPAINP 730 740 750 760 770 780 LLKVMNLVAL LIAPAVIKFS YGADKSVVVR VLIAVVAFAV IAAAVYVSKR RGIAMGDEDD 790 ADPEPKSADP AVVS

References

[1]

"Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. , Hidalgo J., Hornsby T., Howarth S., Huang C.-H., Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D., Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A., Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A. Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL939117 Genomic DNA. Translation: CAB38484.1.
PIR	T36668.
RefSeq	NP_627745.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1098983.
KEGG	sco:SCO3547.
NMPDR	fig|100226.1.peg.3504.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG593668.
OMA	AYLARQY.
Enzyme and pathway databases
BioCyc	SCOE100226:SCO3547-MONOMER.
BRENDA	3.6.1.1. 1084.
Family and domain databases
HAMAP	MF_01130. H_PPase_type2. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_STRCO

Accession

Primary (citable) accession number: Q9X913

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)