Q9X913 (HPPA_STRCO) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 61.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: K(+)-insensitive pyrophosphate-energized proton pump EC=3.6.1.1 Alternative name(s): Membrane-bound proton-translocating pyrophosphatase Pyrophosphate-energized inorganic pyrophosphatase Short name=H(+)-PPase | ||||||
| Gene names |
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| Organism | Streptomyces coelicolor | ||||||
| Taxonomic identifier | 1902 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces |
Protein attributes
| Sequence length | 794 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Proton pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for proton movement across the membrane. Generates a proton motive force. Ref.2 |
| Catalytic activity | Diphosphate + H2O = 2 phosphate. Ref.2 |
| Cofactor | Magnesium By similarity. HAMAP MF_01129 |
| Subunit structure | Homodimer By similarity. HAMAP MF_01129 |
| Subcellular location | Cell membrane; Multi-pass membrane protein By similarity HAMAP MF_01129. |
| Sequence similarities | Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-insensitive subfamily. [View classification] |
Ontologies
| Keywords | |
|---|---|
| Biological process | Hydrogen ion transport Ion transport Transport |
| Cellular component | Cell membrane Membrane |
| Domain | Transmembrane Transmembrane helix |
| Ligand | Magnesium |
| Molecular function | Hydrolase |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological process | proton transport Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membraneInferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 794 | 794 | K(+)-insensitive pyrophosphate-energized proton pump HAMAP MF_01129 | PRO_0000217032 | |||||
Regions | |||||||||
| Transmembrane | 20 – 40 | 21 | Helical; Potential | ||||||
| Transmembrane | 74 – 94 | 21 | Helical; Potential | ||||||
| Transmembrane | 102 – 122 | 21 | Helical; Potential | ||||||
| Transmembrane | 163 – 183 | 21 | Helical; Potential | ||||||
| Transmembrane | 194 – 214 | 21 | Helical; Potential | ||||||
| Transmembrane | 264 – 284 | 21 | Helical; Potential | ||||||
| Transmembrane | 285 – 305 | 21 | Helical; Potential | ||||||
| Transmembrane | 321 – 341 | 21 | Helical; Potential | ||||||
| Transmembrane | 365 – 385 | 21 | Helical; Potential | ||||||
| Transmembrane | 422 – 442 | 21 | Helical; Potential | ||||||
| Transmembrane | 446 – 466 | 21 | Helical; Potential | ||||||
| Transmembrane | 508 – 528 | 21 | Helical; Potential | ||||||
| Transmembrane | 564 – 584 | 21 | Helical; Potential | ||||||
| Transmembrane | 641 – 661 | 21 | Helical; Potential | ||||||
| Transmembrane | 717 – 737 | 21 | Helical; Potential | ||||||
| Transmembrane | 747 – 767 | 21 | Helical; Potential | ||||||
Sites | |||||||||
| Site | 507 | 1 | Determinant of potassium independence By similarity | ||||||
Experimental info | |||||||||
| Mutagenesis | 388 | 1 | F → Y: Increased PPase activity and H(+) pump activity. The coupling ratio is approximately two-fold that of the wild-type. Ref.2 | ||||||
| Mutagenesis | 514 | 1 | A → S: Increased PPase activity and H(+) pump activity. The coupling ratio is approximately two-fold that of the wild-type. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2)." Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L., Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D., Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A., Fraser A., Goble A. Hopwood D.A.Nature 417:141-147(2002) [PubMed: 12000953] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-471 / A3(2) / M145. |
| [2] | "Functional enhancement by single-residue substitution of Streptomyces coelicolor A3(2) H+-translocating pyrophosphatase." Hirono M., Maeshima M. J. Biochem. 146:617-621(2009) [PubMed: 19628678] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, MUTAGENESIS OF PHE-388 AND ALA-514. Strain: A3(2) / NRRL B-16638. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AL939117 Genomic DNA. Translation: CAB38484.1. |
| PIR | T36668. |
| RefSeq | NP_627745.1. NC_003888.3. |
3D structure databases | |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1098983. |
| GenomeReviews | Gene locus SCO3547 in contig AL645882_GR. |
| KEGG | sco:SCO3547. |
| NMPDR | fig|100226.1.peg.3504. |
| PATRIC | 23736838. VBIStrCoe124346_3603. |
Phylogenomic databases | |
| HOGENOM | HBG593668. |
| OMA | AINRGFF. |
| PhylomeDB | Q9X913. |
| ProtClustDB | PRK00733. |
Enzyme and pathway databases | |
| BRENDA | 3.6.1.1. 5998. |
Family and domain databases | |
| HAMAP | MF_01129. PPase-energized_pump. [Tree] |
| InterPro | IPR004131. PPase-energised_H-pump. [Graphical view] |
| KO | K01507. |
| Pfam | PF03030. H_PPase. 1 hit. [Graphical view] |
| PIRSF | PIRSF001265. H+-PPase. 1 hit. |
| TIGRFAMs | TIGR01104. V_PPase. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | HPPA_STRCO | ||||||||
| Accession | Primary (citable) accession number: Q9X913 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| SIMILARITY comments Index of protein domains and families |

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