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Q9X909

- TOP1_STRCO

UniProt

Q9X909 - TOP1_STRCO

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Protein
DNA topoisomerase 1
Gene
topA, SCO3543, SCH5.06c
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi18 – 181Magnesium 1; catalytic By similarity
Sitei42 – 421Interaction with DNA By similarity
Metal bindingi104 – 1041Magnesium 1; catalytic By similarity
Metal bindingi104 – 1041Magnesium 2 By similarity
Metal bindingi106 – 1061Magnesium 2 By similarity
Sitei160 – 1601Interaction with DNA By similarity
Sitei161 – 1611Interaction with DNA By similarity
Sitei164 – 1641Interaction with DNA By similarity
Sitei169 – 1691Interaction with DNA By similarity
Sitei176 – 1761Interaction with DNA By similarity
Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei336 – 3361Interaction with DNA By similarity
Sitei534 – 5341Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:SCO3543
ORF Names:SCH5.06c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 952952DNA topoisomerase 1UniRule annotation
PRO_0000145166Add
BLAST

Proteomic databases

PRIDEiQ9X909.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi100226.SCO3543.

Structurei

3D structure databases

ProteinModelPortaliQ9X909.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 135124Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni184 – 1896Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiFRSAEYW.
OrthoDBiEOG6S7XQ9.
PhylomeDBiQ9X909.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 4 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X909-1 [UniParc]FASTAAdd to Basket

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MSPTSETAKG GRRLVIVESP AKAKTIKGYL GPGYVVEASV GHIRDLPSGA    50
AEVPEKYTGE VRRLGVDVEH DFQPIYVVNA DKKSQVKKLK DLLKESDELF 100
LATDEDREGE AIAWHLQEVL KPKIPVKRMV FHEITKDAIR AAVANPRELN 150
QKLVDAQETR RILDRLYGYE VSPVLWKKVM PRLSAGRVQS VATRLVVERE 200
RERIAFRSAE YWDLTGTFAT GRAGDASDPS SLVARLQTVD GRRVAQGRDF 250
DSLGQLKSAN TLHLDEANAR ALAAALENTR FAVRSVESKP YRRSPYAPFR 300
TTTLQQEASR KLGFGAKSTM QVAQKLYENG YITYMRTDST TLSDTAVSAA 350
RAQVTQLYGA DYLPPQPRTY AGKVKNAQEA HEAIRPSGDR FRTPAETGLT 400
GDQFKLYELI WKRTVASQMK DATGNSVTVK IGGAASDGRD VEFSASGKTI 450
TFHGFLKAYV EGADDPNAEL DDRERRLPQV AEGDALTAEE ITVDGHATKP 500
PARYTEASLV KELEEREIGR PSTYASIIGT ILDRGYVFKK GTALVPSFLS 550
FAVVNLLEKH FGRLVDYDFT ARMEDDLDRI ARGEAQSVPW LRRFYFGEGD 600
GTGGGGAADA GNGDGDHLGG LKELVTDLGA IDAREVSSFP VGNDIKLRVG 650
RYGPYVERGE KDAENHQRAD VPEDLAPDEL SVELAEELLA KPSGDFELGT 700
DPATGHAIVA KDGRYGPYVT EVLPEGTPKT GKNAVKPRTA SLFKSMSLDT 750
VTLDDALKLM SLPRVVGADA EGVEITAQNG RYGPYLKKGT DSRSLQTEDQ 800
LFEITLEEAL AIYAQPKQRG RAAAKPPLKE LGTDPVSEKP VVVKDGRFGP 850
YVTDGETNAT LRSDDSVEEI TPERGYELLA EKRAKGPAKK TAKKAVKKTA 900
AKKAPAKKAA ATKKTAAAKT TAAKKTAAKS TAKKTTAKTA AKKATASKTS 950
ED 952
Length:952
Mass (Da):103,585
Last modified:November 1, 1999 - v1
Checksum:i1C98DABD7A629295
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939116 Genomic DNA. Translation: CAB38480.1.
PIRiT36664.
RefSeqiNP_627741.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB38480; CAB38480; CAB38480.
GeneIDi1098979.
KEGGisco:SCO3543.
PATRICi23736830. VBIStrCoe124346_3599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939116 Genomic DNA. Translation: CAB38480.1 .
PIRi T36664.
RefSeqi NP_627741.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9X909.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO3543.

Proteomic databases

PRIDEi Q9X909.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB38480 ; CAB38480 ; CAB38480 .
GeneIDi 1098979.
KEGGi sco:SCO3543.
PATRICi 23736830. VBIStrCoe124346_3599.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi FRSAEYW.
OrthoDBi EOG6S7XQ9.
PhylomeDBi Q9X909.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 4 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiTOP1_STRCO
AccessioniPrimary (citable) accession number: Q9X909
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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