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Q9X909

- TOP1_STRCO

UniProt

Q9X909 - TOP1_STRCO

Protein

DNA topoisomerase 1

Gene

topA

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 100 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi18 – 181Magnesium 1; catalyticUniRule annotation
    Sitei42 – 421Interaction with DNAUniRule annotation
    Metal bindingi104 – 1041Magnesium 1; catalyticUniRule annotation
    Metal bindingi104 – 1041Magnesium 2UniRule annotation
    Metal bindingi106 – 1061Magnesium 2UniRule annotation
    Sitei160 – 1601Interaction with DNAUniRule annotation
    Sitei161 – 1611Interaction with DNAUniRule annotation
    Sitei164 – 1641Interaction with DNAUniRule annotation
    Sitei169 – 1691Interaction with DNAUniRule annotation
    Sitei176 – 1761Interaction with DNAUniRule annotation
    Active sitei334 – 3341O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei336 – 3361Interaction with DNAUniRule annotation
    Sitei534 – 5341Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:SCO3543
    ORF Names:SCH5.06c
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 952952DNA topoisomerase 1PRO_0000145166Add
    BLAST

    Proteomic databases

    PRIDEiQ9X909.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi100226.SCO3543.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9X909.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini12 – 135124ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni184 – 1896Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1754.
    HOGENOMiHOG000004020.
    KOiK03168.
    OMAiFRSAEYW.
    OrthoDBiEOG6S7XQ9.
    PhylomeDBiQ9X909.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 4 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X909-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSPTSETAKG GRRLVIVESP AKAKTIKGYL GPGYVVEASV GHIRDLPSGA    50
    AEVPEKYTGE VRRLGVDVEH DFQPIYVVNA DKKSQVKKLK DLLKESDELF 100
    LATDEDREGE AIAWHLQEVL KPKIPVKRMV FHEITKDAIR AAVANPRELN 150
    QKLVDAQETR RILDRLYGYE VSPVLWKKVM PRLSAGRVQS VATRLVVERE 200
    RERIAFRSAE YWDLTGTFAT GRAGDASDPS SLVARLQTVD GRRVAQGRDF 250
    DSLGQLKSAN TLHLDEANAR ALAAALENTR FAVRSVESKP YRRSPYAPFR 300
    TTTLQQEASR KLGFGAKSTM QVAQKLYENG YITYMRTDST TLSDTAVSAA 350
    RAQVTQLYGA DYLPPQPRTY AGKVKNAQEA HEAIRPSGDR FRTPAETGLT 400
    GDQFKLYELI WKRTVASQMK DATGNSVTVK IGGAASDGRD VEFSASGKTI 450
    TFHGFLKAYV EGADDPNAEL DDRERRLPQV AEGDALTAEE ITVDGHATKP 500
    PARYTEASLV KELEEREIGR PSTYASIIGT ILDRGYVFKK GTALVPSFLS 550
    FAVVNLLEKH FGRLVDYDFT ARMEDDLDRI ARGEAQSVPW LRRFYFGEGD 600
    GTGGGGAADA GNGDGDHLGG LKELVTDLGA IDAREVSSFP VGNDIKLRVG 650
    RYGPYVERGE KDAENHQRAD VPEDLAPDEL SVELAEELLA KPSGDFELGT 700
    DPATGHAIVA KDGRYGPYVT EVLPEGTPKT GKNAVKPRTA SLFKSMSLDT 750
    VTLDDALKLM SLPRVVGADA EGVEITAQNG RYGPYLKKGT DSRSLQTEDQ 800
    LFEITLEEAL AIYAQPKQRG RAAAKPPLKE LGTDPVSEKP VVVKDGRFGP 850
    YVTDGETNAT LRSDDSVEEI TPERGYELLA EKRAKGPAKK TAKKAVKKTA 900
    AKKAPAKKAA ATKKTAAAKT TAAKKTAAKS TAKKTTAKTA AKKATASKTS 950
    ED 952
    Length:952
    Mass (Da):103,585
    Last modified:November 1, 1999 - v1
    Checksum:i1C98DABD7A629295
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939116 Genomic DNA. Translation: CAB38480.1.
    PIRiT36664.
    RefSeqiNP_627741.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB38480; CAB38480; CAB38480.
    GeneIDi1098979.
    KEGGisco:SCO3543.
    PATRICi23736830. VBIStrCoe124346_3599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939116 Genomic DNA. Translation: CAB38480.1 .
    PIRi T36664.
    RefSeqi NP_627741.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali Q9X909.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO3543.

    Proteomic databases

    PRIDEi Q9X909.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB38480 ; CAB38480 ; CAB38480 .
    GeneIDi 1098979.
    KEGGi sco:SCO3543.
    PATRICi 23736830. VBIStrCoe124346_3599.

    Phylogenomic databases

    eggNOGi COG1754.
    HOGENOMi HOG000004020.
    KOi K03168.
    OMAi FRSAEYW.
    OrthoDBi EOG6S7XQ9.
    PhylomeDBi Q9X909.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 4 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiTOP1_STRCO
    AccessioniPrimary (citable) accession number: Q9X909
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 100 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3