ID   SSB2_STRCO              Reviewed;         199 AA.
AC   Q9X8U3;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   27-MAR-2024, entry version 127.
DE   RecName: Full=Single-stranded DNA-binding protein 2 {ECO:0000255|HAMAP-Rule:MF_00984};
DE            Short=SSB 2 {ECO:0000255|HAMAP-Rule:MF_00984};
GN   Name=ssb2; OrderedLocusNames=SCO3907; ORFNames=SCH24.29;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC   Streptomycetaceae; Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   PHOSPHORYLATION.
RX   PubMed=16549871; DOI=10.1093/nar/gkj514;
RA   Mijakovic I., Petranovic D., Macek B., Cepo T., Mann M., Davies J.,
RA   Jensen P.R., Vujaklija D.;
RT   "Bacterial single-stranded DNA-binding proteins are phosphorylated on
RT   tyrosine.";
RL   Nucleic Acids Res. 34:1588-1596(2006).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=23523638; DOI=10.1016/j.jprot.2013.02.033;
RA   Bradshaw E., Saalbach G., McArthur M.;
RT   "Proteomic survey of the Streptomyces coelicolor nucleoid.";
RL   J. Proteomics 83:37-46(2013).
RN   [4] {ECO:0007744|PDB:3EIV}
RP   X-RAY CRYSTALLOGRAPHY (2.14 ANGSTROMS), SUBUNIT, AND DOMAIN.
RX   PubMed=19690375; DOI=10.1107/s0907444909023634;
RA   Stefanic Z., Vujaklija D., Luic M.;
RT   "Structure of the single-stranded DNA-binding protein from Streptomyces
RT   coelicolor.";
RL   Acta Crystallogr. D 65:974-979(2009).
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00984,
CC       ECO:0000269|PubMed:19690375}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000305|PubMed:23523638}.
CC   -!- DOMAIN: The N-terminal 120 residues form an OB-fold.
CC       {ECO:0000269|PubMed:19690375}.
CC   -!- PTM: Phosphorylated on tyrosine residue(s) when expressed in E.coli.
CC       {ECO:0000269|PubMed:16549871}.
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DR   EMBL; AL939118; CAB42735.1; -; Genomic_DNA.
DR   PIR; T36594; T36594.
DR   RefSeq; NP_628093.1; NC_003888.3.
DR   RefSeq; WP_011029303.1; NZ_VNID01000003.1.
DR   PDB; 3EIV; X-ray; 2.14 A; A/B/C/D=1-199.
DR   PDBsum; 3EIV; -.
DR   AlphaFoldDB; Q9X8U3; -.
DR   SMR; Q9X8U3; -.
DR   STRING; 100226.gene:17761534; -.
DR   PaxDb; 100226-SCO3907; -.
DR   PATRIC; fig|100226.15.peg.3981; -.
DR   eggNOG; COG0629; Bacteria.
DR   HOGENOM; CLU_078758_1_0_11; -.
DR   InParanoid; Q9X8U3; -.
DR   OrthoDB; 9809878at2; -.
DR   PhylomeDB; Q9X8U3; -.
DR   EvolutionaryTrace; Q9X8U3; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   CDD; cd04496; SSB_OBF; 1.
DR   DisProt; DP00854; -.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   HAMAP; MF_00984; SSB; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR000424; Primosome_PriB/ssb.
DR   InterPro; IPR011344; ssDNA-bd.
DR   NCBIfam; TIGR00621; ssb; 1.
DR   PANTHER; PTHR10302; SINGLE-STRANDED DNA-BINDING PROTEIN; 1.
DR   PANTHER; PTHR10302:SF27; SINGLE-STRANDED DNA-BINDING PROTEIN; 1.
DR   Pfam; PF00436; SSB; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS50935; SSB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA-binding; Phosphoprotein; Reference proteome.
FT   CHAIN           1..199
FT                   /note="Single-stranded DNA-binding protein 2"
FT                   /id="PRO_0000096116"
FT   DOMAIN          1..110
FT                   /note="SSB"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT   REGION          114..199
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          6..15
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          18..21
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          27..35
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   TURN            42..45
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          53..60
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   HELIX           62..70
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          76..86
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          98..109
FT                   /evidence="ECO:0007829|PDB:3EIV"
FT   STRAND          111..119
FT                   /evidence="ECO:0007829|PDB:3EIV"
SQ   SEQUENCE   199 AA;  19907 MW;  CB291962A445AA5F CRC64;
     MAGETVITVV GNLVDDPELR FTPSGAAVAK FRVASTPRTF DRQTNEWKDG ESLFLTCSVW
     RQAAENVAES LQRGMRVIVQ GRLKQRSYED REGVKRTVYE LDVDEVGASL RSATAKVTKT
     SGQGRGGQGG YGGGGGGQGG GGWGGGPGGG QQGGGAPADD PWATGGAPAG GQQGGGGQGG
     GGWGGGSGGG GGYSDEPPF
//