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Q9X839

- CYC2_STRCO

UniProt

Q9X839 - CYC2_STRCO

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Protein

Germacradienol/geosmin synthase

Gene
cyc2, SCO6073, SC9B1.20
Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Tow-domain protein where the N-terminal domain catalyzes the cyclization of farnesyl diphosphate (FPP) to a 85:15 mixture of the sesquiterpene alcohol germacradienol and the sesquiterpene hydrocarbon germacrene D. The C-terminal domain partially converts the germacradienol formed into geosmin, the characteristic odoriferous ('earthy aroma') constituent of Streptomyces species.4 Publications

Catalytic activityi

(2E,6E)-farnesyl diphosphate + H2O = (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate.1 Publication
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (-)-geosmin + acetone.1 Publication
(2E,6E)-farnesyl diphosphate = (-)-germacrene D + diphosphate.1 Publication

Cofactori

Magnesium. Fe2+ or Cu2+ ions are very less efficient as cofactors.2 Publications

Kineticsi

  1. KM=62 nM for FPP1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861Magnesium Reviewed prediction
Metal bindingi91 – 911Magnesium Reviewed prediction
Metal bindingi267 – 2671Magnesium Reviewed prediction
Metal bindingi271 – 2711Magnesium Reviewed prediction
Metal bindingi276 – 2761Magnesium Reviewed prediction
Metal bindingi455 – 4551Magnesium Reviewed prediction
Metal bindingi598 – 5981Magnesium Reviewed prediction
Metal bindingi602 – 6021Magnesium Reviewed prediction
Metal bindingi606 – 6061Magnesium Reviewed prediction

GO - Molecular functioni

  1. germacradienol synthase activity Source: UniProtKB-EC
  2. germacrene-D synthase activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14022.
BRENDAi4.2.3.22. 5998.
SABIO-RKQ9X839.
UniPathwayiUPA00209.
UPA00283; UER00583.
UPA00285; UER00584.

Names & Taxonomyi

Protein namesi
Recommended name:
Germacradienol/geosmin synthase
Including the following 2 domains:
Germacradienol/germacrene D synthase (EC:4.2.3.22, EC:4.2.3.75)
Alternative name(s):
Sesquiterpene cyclase
Sesquiterpene synthase
Geosmin synthase (EC:4.1.99.16)
Gene namesi
Name:cyc2
Ordered Locus Names:SCO6073
ORF Names:SC9B1.20
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 726725Germacradienol/geosmin synthasePRO_0000247895Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO6073.

Structurei

3D structure databases

ProteinModelPortaliQ9X839.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 354353Germacradienol/germacrene D synthaseAdd
BLAST
Regioni355 – 726372Geosmin synthaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 916DDXXD motif 1; degenerate
Motifi455 – 4595DDXXD motif 2; degenerate

Domaini

Consists of 2 homologous sesquiterpene synthase domains. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.2 Publications

Sequence similaritiesi

Belongs to the terpene synthase family.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG15313.
HOGENOMiHOG000253477.
KOiK10187.
OMAiRLHQFEH.
OrthoDBiEOG622PK5.

Family and domain databases

Gene3Di1.10.600.10. 2 hits.
InterProiIPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 2 hits.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9X839-1 [UniParc]FASTAAdd to Basket

« Hide

MTQQPFQLPH FYLPHPARLN PHLDEARAHS TTWAREMGML EGSGVWEQSD    50
LEAHDYGLLC AYTHPDCDGP ALSLITDWYV WVFFFDDHFL EKYKRSQDRL 100
AGKAHLDRLP LFMPLDDAAG MPEPRNPVEA GLADLWTRTV PAMSADWRRR 150
FAVATEHLLN ESMWELSNIN EGRVANPVEY IEMRRKVGGA PWSAGLVEYA 200
TAEVPAAVAG TRPLRVLMET FSDAVHLRND LFSYQREVED EGELSNGVLV 250
LETFFGCTTQ EAADLVNDVL TSRLHQFEHT AFTEVPAVAL EKGLTPLEVA 300
AVGAYTKGLQ DWQSGGHEWH MRSSRYMNKG ERPLAGWQAL TGPGTSAADV 350
GALLADAVAQ RARSYTYVPF QKVGPSVIPD IRMPYPLELS PALDGARRHL 400
SEWCREMGIL SEGVWDEDKL ESCDLPLCAA GLDPDATQDQ LDLASGWLAF 450
GTYGDDYYPL VYGHRRDLAA ARLTTTRLSD CMPLDGEPVP PPGNAMERSL 500
IDLWVRTTAG MTPEERRPLK KAVDDMTEAW LWELSNQIQN RVPDPVDYLE 550
MRRATFGSDL TLGLCRAGHG PAVPPEVYRS GPVRSLENAA IDYACLLNDV 600
FSYQKEIEYE GEIHNAVLVV QNFFGVDYPA ALGVVQDLMN QRMRQFEHVV 650
AHELPVVYDD FQLSEEARTV MRGYVTDLQN WMAGILNWHR NVPRYKAEYL 700
AGRTHGFLPD RIPAPPVPRS SPALTH 726
Length:726
Mass (Da):81,474
Last modified:January 23, 2007 - v3
Checksum:iCB332AC7840EB81E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939126 Genomic DNA. Translation: CAB41566.1.
PIRiT35865.
RefSeqiNP_630182.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB41566; CAB41566; CAB41566.
GeneIDi1101514.
KEGGisco:SCO6073.
PATRICi23742056. VBIStrCoe124346_6175.

Cross-referencesi

Web resourcesi

Protein Spotlight

The earth's perfume - Issue 35 of June 2003

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL939126 Genomic DNA. Translation: CAB41566.1 .
PIRi T35865.
RefSeqi NP_630182.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9X839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO6073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB41566 ; CAB41566 ; CAB41566 .
GeneIDi 1101514.
KEGGi sco:SCO6073.
PATRICi 23742056. VBIStrCoe124346_6175.

Phylogenomic databases

eggNOGi NOG15313.
HOGENOMi HOG000253477.
KOi K10187.
OMAi RLHQFEH.
OrthoDBi EOG622PK5.

Enzyme and pathway databases

UniPathwayi UPA00209 .
UPA00283 ; UER00583 .
UPA00285 ; UER00584 .
BioCyci MetaCyc:MONOMER-14022.
BRENDAi 4.2.3.22. 5998.
SABIO-RK Q9X839.

Family and domain databases

Gene3Di 1.10.600.10. 2 hits.
InterProi IPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF03936. Terpene_synth_C. 2 hits.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis."
    Cane D.E., Watt R.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:1547-1551(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10, FUNCTION, CATALYTIC ACTIVITY, DOMAIN, KINETIC PARAMETERS, COFACTOR.
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin."
    Gust B., Challis G.L., Fowler K., Kieser T., Chater K.F.
    Proc. Natl. Acad. Sci. U.S.A. 100:1541-1546(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, ROLE IN GEOSMIN BIOSYNTHESIS.
    Strain: ATCC BAA-471 / A3(2) / M145.
  4. "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)."
    He X., Cane D.E.
    J. Am. Chem. Soc. 126:2678-2679(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GERMACRENE D PRODUCTION, REACTION STEREOCHEMISTRY.
    Strain: ATCC BAA-471 / A3(2) / M145.
  5. "Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin."
    Jiang J., He X., Cane D.E.
    J. Am. Chem. Soc. 128:8128-8129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DIRECT GEOSMIN PRODUCTION, COFACTOR, REACTION MECHANISM.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiCYC2_STRCO
AccessioniPrimary (citable) accession number: Q9X839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 23, 2007
Last modified: May 14, 2014
This is version 82 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The earthy odorant geosmin is also responsible for the 'off-flavor' of contaminated drinking water, wines, and other foodstuffs.

Caution

It is uncertain whether the initial Met is cleaved or not.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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