Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9X839

- CYC2_STRCO

UniProt

Q9X839 - CYC2_STRCO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Germacradienol/geosmin synthase

Gene

cyc2

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Tow-domain protein where the N-terminal domain catalyzes the cyclization of farnesyl diphosphate (FPP) to a 85:15 mixture of the sesquiterpene alcohol germacradienol and the sesquiterpene hydrocarbon germacrene D. The C-terminal domain partially converts the germacradienol formed into geosmin, the characteristic odoriferous ('earthy aroma') constituent of Streptomyces species.4 Publications

Catalytic activityi

(2E,6E)-farnesyl diphosphate + H2O = (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate.1 Publication
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (-)-geosmin + acetone.1 Publication
(2E,6E)-farnesyl diphosphate = (-)-germacrene D + diphosphate.1 Publication

Cofactori

Mg2+2 PublicationsNote: Magnesium. Fe(2+) or Cu(2+) ions are very less efficient as cofactors.2 Publications

Kineticsi

  1. KM=62 nM for FPP1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi86 – 861MagnesiumSequence Analysis
Metal bindingi91 – 911MagnesiumSequence Analysis
Metal bindingi267 – 2671MagnesiumSequence Analysis
Metal bindingi271 – 2711MagnesiumSequence Analysis
Metal bindingi276 – 2761MagnesiumSequence Analysis
Metal bindingi455 – 4551MagnesiumSequence Analysis
Metal bindingi598 – 5981MagnesiumSequence Analysis
Metal bindingi602 – 6021MagnesiumSequence Analysis
Metal bindingi606 – 6061MagnesiumSequence Analysis

GO - Molecular functioni

  1. germacradienol synthase activity Source: UniProtKB-EC
  2. germacrene-D synthase activity Source: UniProtKB-EC
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14022.
BRENDAi4.2.3.22. 5998.
SABIO-RKQ9X839.
UniPathwayiUPA00209.
UPA00283; UER00583.
UPA00285; UER00584.

Names & Taxonomyi

Protein namesi
Recommended name:
Germacradienol/geosmin synthase
Including the following 2 domains:
Germacradienol/germacrene D synthase (EC:4.2.3.22, EC:4.2.3.75)
Alternative name(s):
Sesquiterpene cyclase
Sesquiterpene synthase
Geosmin synthase (EC:4.1.99.16)
Gene namesi
Name:cyc2
Ordered Locus Names:SCO6073
ORF Names:SC9B1.20
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
ProteomesiUP000001973: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 726725Germacradienol/geosmin synthasePRO_0000247895Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi100226.SCO6073.

Structurei

3D structure databases

ProteinModelPortaliQ9X839.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 354353Germacradienol/germacrene D synthaseAdd
BLAST
Regioni355 – 726372Geosmin synthaseAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi86 – 916DDXXD motif 1; degenerate
Motifi455 – 4595DDXXD motif 2; degenerate

Domaini

Consists of 2 homologous sesquiterpene synthase domains. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.2 Publications

Sequence similaritiesi

Belongs to the terpene synthase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG15313.
HOGENOMiHOG000253477.
InParanoidiQ9X839.
KOiK10187.
OMAiRLHQFEH.
OrthoDBiEOG622PK5.

Family and domain databases

Gene3Di1.10.600.10. 2 hits.
InterProiIPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF03936. Terpene_synth_C. 2 hits.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9X839-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQQPFQLPH FYLPHPARLN PHLDEARAHS TTWAREMGML EGSGVWEQSD
60 70 80 90 100
LEAHDYGLLC AYTHPDCDGP ALSLITDWYV WVFFFDDHFL EKYKRSQDRL
110 120 130 140 150
AGKAHLDRLP LFMPLDDAAG MPEPRNPVEA GLADLWTRTV PAMSADWRRR
160 170 180 190 200
FAVATEHLLN ESMWELSNIN EGRVANPVEY IEMRRKVGGA PWSAGLVEYA
210 220 230 240 250
TAEVPAAVAG TRPLRVLMET FSDAVHLRND LFSYQREVED EGELSNGVLV
260 270 280 290 300
LETFFGCTTQ EAADLVNDVL TSRLHQFEHT AFTEVPAVAL EKGLTPLEVA
310 320 330 340 350
AVGAYTKGLQ DWQSGGHEWH MRSSRYMNKG ERPLAGWQAL TGPGTSAADV
360 370 380 390 400
GALLADAVAQ RARSYTYVPF QKVGPSVIPD IRMPYPLELS PALDGARRHL
410 420 430 440 450
SEWCREMGIL SEGVWDEDKL ESCDLPLCAA GLDPDATQDQ LDLASGWLAF
460 470 480 490 500
GTYGDDYYPL VYGHRRDLAA ARLTTTRLSD CMPLDGEPVP PPGNAMERSL
510 520 530 540 550
IDLWVRTTAG MTPEERRPLK KAVDDMTEAW LWELSNQIQN RVPDPVDYLE
560 570 580 590 600
MRRATFGSDL TLGLCRAGHG PAVPPEVYRS GPVRSLENAA IDYACLLNDV
610 620 630 640 650
FSYQKEIEYE GEIHNAVLVV QNFFGVDYPA ALGVVQDLMN QRMRQFEHVV
660 670 680 690 700
AHELPVVYDD FQLSEEARTV MRGYVTDLQN WMAGILNWHR NVPRYKAEYL
710 720
AGRTHGFLPD RIPAPPVPRS SPALTH
Length:726
Mass (Da):81,474
Last modified:January 23, 2007 - v3
Checksum:iCB332AC7840EB81E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939126 Genomic DNA. Translation: CAB41566.1.
PIRiT35865.
RefSeqiNP_630182.1. NC_003888.3.
WP_011030632.1. NC_003888.3.

Genome annotation databases

EnsemblBacteriaiCAB41566; CAB41566; CAB41566.
GeneIDi1101514.
KEGGisco:SCO6073.
PATRICi23742056. VBIStrCoe124346_6175.

Cross-referencesi

Web resourcesi

Protein Spotlight

The earth's perfume - Issue 35 of June 2003

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939126 Genomic DNA. Translation: CAB41566.1 .
PIRi T35865.
RefSeqi NP_630182.1. NC_003888.3.
WP_011030632.1. NC_003888.3.

3D structure databases

ProteinModelPortali Q9X839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 100226.SCO6073.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB41566 ; CAB41566 ; CAB41566 .
GeneIDi 1101514.
KEGGi sco:SCO6073.
PATRICi 23742056. VBIStrCoe124346_6175.

Phylogenomic databases

eggNOGi NOG15313.
HOGENOMi HOG000253477.
InParanoidi Q9X839.
KOi K10187.
OMAi RLHQFEH.
OrthoDBi EOG622PK5.

Enzyme and pathway databases

UniPathwayi UPA00209 .
UPA00283 ; UER00583 .
UPA00285 ; UER00584 .
BioCyci MetaCyc:MONOMER-14022.
BRENDAi 4.2.3.22. 5998.
SABIO-RK Q9X839.

Family and domain databases

Gene3Di 1.10.600.10. 2 hits.
InterProi IPR005630. Terpene_synthase_metal-bd.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF03936. Terpene_synth_C. 2 hits.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 2 hits.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-471 / A3(2) / M145.
  2. "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis."
    Cane D.E., Watt R.M.
    Proc. Natl. Acad. Sci. U.S.A. 100:1547-1551(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-10, FUNCTION, CATALYTIC ACTIVITY, DOMAIN, KINETIC PARAMETERS, COFACTOR.
    Strain: ATCC BAA-471 / A3(2) / M145.
  3. "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin."
    Gust B., Challis G.L., Fowler K., Kieser T., Chater K.F.
    Proc. Natl. Acad. Sci. U.S.A. 100:1541-1546(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, ROLE IN GEOSMIN BIOSYNTHESIS.
    Strain: ATCC BAA-471 / A3(2) / M145.
  4. "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)."
    He X., Cane D.E.
    J. Am. Chem. Soc. 126:2678-2679(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN GERMACRENE D PRODUCTION, REACTION STEREOCHEMISTRY.
    Strain: ATCC BAA-471 / A3(2) / M145.
  5. "Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin."
    Jiang J., He X., Cane D.E.
    J. Am. Chem. Soc. 128:8128-8129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DIRECT GEOSMIN PRODUCTION, COFACTOR, REACTION MECHANISM.
    Strain: ATCC BAA-471 / A3(2) / M145.

Entry informationi

Entry nameiCYC2_STRCO
AccessioniPrimary (citable) accession number: Q9X839
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The earthy odorant geosmin is also responsible for the 'off-flavor' of contaminated drinking water, wines, and other foodstuffs.

Caution

It is uncertain whether the initial Met is cleaved or not.Curated

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3