Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q9X839

- CYC2_STRCO

UniProt

Q9X839 - CYC2_STRCO

Protein

Germacradienol/geosmin synthase

Gene

cyc2

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Tow-domain protein where the N-terminal domain catalyzes the cyclization of farnesyl diphosphate (FPP) to a 85:15 mixture of the sesquiterpene alcohol germacradienol and the sesquiterpene hydrocarbon germacrene D. The C-terminal domain partially converts the germacradienol formed into geosmin, the characteristic odoriferous ('earthy aroma') constituent of Streptomyces species.4 Publications

    Catalytic activityi

    (2E,6E)-farnesyl diphosphate + H2O = (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + diphosphate.1 Publication
    (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (-)-geosmin + acetone.1 Publication
    (2E,6E)-farnesyl diphosphate = (-)-germacrene D + diphosphate.1 Publication

    Cofactori

    Magnesium. Fe2+ or Cu2+ ions are very less efficient as cofactors.2 Publications

    Kineticsi

    1. KM=62 nM for FPP1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi86 – 861MagnesiumSequence Analysis
    Metal bindingi91 – 911MagnesiumSequence Analysis
    Metal bindingi267 – 2671MagnesiumSequence Analysis
    Metal bindingi271 – 2711MagnesiumSequence Analysis
    Metal bindingi276 – 2761MagnesiumSequence Analysis
    Metal bindingi455 – 4551MagnesiumSequence Analysis
    Metal bindingi598 – 5981MagnesiumSequence Analysis
    Metal bindingi602 – 6021MagnesiumSequence Analysis
    Metal bindingi606 – 6061MagnesiumSequence Analysis

    GO - Molecular functioni

    1. germacradienol synthase activity Source: UniProtKB-EC
    2. germacrene-D synthase activity Source: UniProtKB-EC
    3. magnesium ion binding Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-14022.
    BRENDAi4.2.3.22. 5998.
    SABIO-RKQ9X839.
    UniPathwayiUPA00209.
    UPA00283; UER00583.
    UPA00285; UER00584.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Germacradienol/geosmin synthase
    Including the following 2 domains:
    Germacradienol/germacrene D synthase (EC:4.2.3.22, EC:4.2.3.75)
    Alternative name(s):
    Sesquiterpene cyclase
    Sesquiterpene synthase
    Geosmin synthase (EC:4.1.99.16)
    Gene namesi
    Name:cyc2
    Ordered Locus Names:SCO6073
    ORF Names:SC9B1.20
    OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
    Taxonomic identifieri100226 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
    ProteomesiUP000001973: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 726725Germacradienol/geosmin synthasePRO_0000247895Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi100226.SCO6073.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9X839.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 354353Germacradienol/germacrene D synthaseAdd
    BLAST
    Regioni355 – 726372Geosmin synthaseAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi86 – 916DDXXD motif 1; degenerate
    Motifi455 – 4595DDXXD motif 2; degenerate

    Domaini

    Consists of 2 homologous sesquiterpene synthase domains. The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg2+.2 Publications

    Sequence similaritiesi

    Belongs to the terpene synthase family.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG15313.
    HOGENOMiHOG000253477.
    KOiK10187.
    OMAiRLHQFEH.
    OrthoDBiEOG622PK5.

    Family and domain databases

    Gene3Di1.10.600.10. 2 hits.
    InterProiIPR005630. Terpene_synthase_metal-bd.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF03936. Terpene_synth_C. 2 hits.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9X839-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQQPFQLPH FYLPHPARLN PHLDEARAHS TTWAREMGML EGSGVWEQSD    50
    LEAHDYGLLC AYTHPDCDGP ALSLITDWYV WVFFFDDHFL EKYKRSQDRL 100
    AGKAHLDRLP LFMPLDDAAG MPEPRNPVEA GLADLWTRTV PAMSADWRRR 150
    FAVATEHLLN ESMWELSNIN EGRVANPVEY IEMRRKVGGA PWSAGLVEYA 200
    TAEVPAAVAG TRPLRVLMET FSDAVHLRND LFSYQREVED EGELSNGVLV 250
    LETFFGCTTQ EAADLVNDVL TSRLHQFEHT AFTEVPAVAL EKGLTPLEVA 300
    AVGAYTKGLQ DWQSGGHEWH MRSSRYMNKG ERPLAGWQAL TGPGTSAADV 350
    GALLADAVAQ RARSYTYVPF QKVGPSVIPD IRMPYPLELS PALDGARRHL 400
    SEWCREMGIL SEGVWDEDKL ESCDLPLCAA GLDPDATQDQ LDLASGWLAF 450
    GTYGDDYYPL VYGHRRDLAA ARLTTTRLSD CMPLDGEPVP PPGNAMERSL 500
    IDLWVRTTAG MTPEERRPLK KAVDDMTEAW LWELSNQIQN RVPDPVDYLE 550
    MRRATFGSDL TLGLCRAGHG PAVPPEVYRS GPVRSLENAA IDYACLLNDV 600
    FSYQKEIEYE GEIHNAVLVV QNFFGVDYPA ALGVVQDLMN QRMRQFEHVV 650
    AHELPVVYDD FQLSEEARTV MRGYVTDLQN WMAGILNWHR NVPRYKAEYL 700
    AGRTHGFLPD RIPAPPVPRS SPALTH 726
    Length:726
    Mass (Da):81,474
    Last modified:January 23, 2007 - v3
    Checksum:iCB332AC7840EB81E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939126 Genomic DNA. Translation: CAB41566.1.
    PIRiT35865.
    RefSeqiNP_630182.1. NC_003888.3.

    Genome annotation databases

    EnsemblBacteriaiCAB41566; CAB41566; CAB41566.
    GeneIDi1101514.
    KEGGisco:SCO6073.
    PATRICi23742056. VBIStrCoe124346_6175.

    Cross-referencesi

    Web resourcesi

    Protein Spotlight

    The earth's perfume - Issue 35 of June 2003

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL939126 Genomic DNA. Translation: CAB41566.1 .
    PIRi T35865.
    RefSeqi NP_630182.1. NC_003888.3.

    3D structure databases

    ProteinModelPortali Q9X839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 100226.SCO6073.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB41566 ; CAB41566 ; CAB41566 .
    GeneIDi 1101514.
    KEGGi sco:SCO6073.
    PATRICi 23742056. VBIStrCoe124346_6175.

    Phylogenomic databases

    eggNOGi NOG15313.
    HOGENOMi HOG000253477.
    KOi K10187.
    OMAi RLHQFEH.
    OrthoDBi EOG622PK5.

    Enzyme and pathway databases

    UniPathwayi UPA00209 .
    UPA00283 ; UER00583 .
    UPA00285 ; UER00584 .
    BioCyci MetaCyc:MONOMER-14022.
    BRENDAi 4.2.3.22. 5998.
    SABIO-RK Q9X839.

    Family and domain databases

    Gene3Di 1.10.600.10. 2 hits.
    InterProi IPR005630. Terpene_synthase_metal-bd.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF03936. Terpene_synth_C. 2 hits.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-471 / A3(2) / M145.
    2. "Expression and mechanistic analysis of a germacradienol synthase from Streptomyces coelicolor implicated in geosmin biosynthesis."
      Cane D.E., Watt R.M.
      Proc. Natl. Acad. Sci. U.S.A. 100:1547-1551(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-10, FUNCTION, CATALYTIC ACTIVITY, DOMAIN, KINETIC PARAMETERS, COFACTOR.
      Strain: ATCC BAA-471 / A3(2) / M145.
    3. "PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin."
      Gust B., Challis G.L., Fowler K., Kieser T., Chater K.F.
      Proc. Natl. Acad. Sci. U.S.A. 100:1541-1546(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN, ROLE IN GEOSMIN BIOSYNTHESIS.
      Strain: ATCC BAA-471 / A3(2) / M145.
    4. "Mechanism and stereochemistry of the germacradienol/germacrene D synthase of Streptomyces coelicolor A3(2)."
      He X., Cane D.E.
      J. Am. Chem. Soc. 126:2678-2679(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN GERMACRENE D PRODUCTION, REACTION STEREOCHEMISTRY.
      Strain: ATCC BAA-471 / A3(2) / M145.
    5. "Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin."
      Jiang J., He X., Cane D.E.
      J. Am. Chem. Soc. 128:8128-8129(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DIRECT GEOSMIN PRODUCTION, COFACTOR, REACTION MECHANISM.
      Strain: ATCC BAA-471 / A3(2) / M145.

    Entry informationi

    Entry nameiCYC2_STRCO
    AccessioniPrimary (citable) accession number: Q9X839
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 25, 2006
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 83 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The earthy odorant geosmin is also responsible for the 'off-flavor' of contaminated drinking water, wines, and other foodstuffs.

    Caution

    It is uncertain whether the initial Met is cleaved or not.Curated

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Protein Spotlight
      Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3