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Protein

L-threonine dehydratase biosynthetic IlvA

Gene

ilvA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA (By similarity).By similarity

Catalytic activityi

L-threonine = 2-oxobutanoate + NH3.

Cofactori

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 2-oxobutanoate from L-threonine.
Proteins known to be involved in this subpathway in this organism are:
  1. L-threonine dehydratase biosynthetic IlvA (ilvA)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-oxobutanoate from L-threonine, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei90Pyridoxal phosphateBy similarity1
Binding sitei319Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Isoleucine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-1319-MONOMER
UniPathwayiUPA00047; UER00054

Names & Taxonomyi

Protein namesi
Recommended name:
L-threonine dehydratase biosynthetic IlvA (EC:4.3.1.19)
Alternative name(s):
Threonine deaminase
Gene namesi
Name:ilvA
Ordered Locus Names:ML1209
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1209

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001855761 – 427L-threonine dehydratase biosynthetic IlvAAdd BLAST427

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi272631.ML1209

Structurei

3D structure databases

ProteinModelPortaliQ9X7F1
SMRiQ9X7F1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini343 – 417ACT-likePROSITE-ProRule annotationAdd BLAST75

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni193 – 197Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C7B Bacteria
COG1171 LUCA
HOGENOMiHOG000046973
KOiK01754
OMAiRNHGAAY
OrthoDBiPOG091H03MP

Family and domain databases

Gene3Di3.40.1020.10, 1 hit
InterProiView protein in InterPro
IPR011820 IlvA
IPR001926 PLP-dep
IPR000634 Ser/Thr_deHydtase_PyrdxlP-BS
IPR001721 TD_ACT-like
IPR038110 TD_ACT-like_sf
IPR036052 Trypto_synt_PLP_dependent
PfamiView protein in Pfam
PF00291 PALP, 1 hit
PF00585 Thr_dehydrat_C, 1 hit
SUPFAMiSSF53686 SSF53686, 1 hit
TIGRFAMsiTIGR02079 THD1, 1 hit
PROSITEiView protein in PROSITE
PS51672 ACT_LIKE, 1 hit
PS00165 DEHYDRATASE_SER_THR, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X7F1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAEPSRNPR TPPVSAVDID GAAKRIAPVV TPTPLQLSDR LSAITGAAVY
60 70 80 90 100
LKREDLQTVR SYKLRGAYNL LVQLTDEEIA AGVVCSSAGN HAQGVAYACR
110 120 130 140 150
SLGVHGRVYV PAKTPKQKWD RIRYHGGAFI ELIVGRSTYD LAAAAAVDDI
160 170 180 190 200
ERTGATLVPP YDDVRVIAGQ GTIAVELLEQ LNTEPDLVVV PVGGGGCIAG
210 220 230 240 250
MTTYLAERTA NTAVLGVEPA GAAAMMAALA AGEPVTLDYV DQFVDGAAVN
260 270 280 290 300
RVGTLPYAAL TAAGDMVSIT TVDEGAVCTA MLDLYQNEGI IAEPAGALSV
310 320 330 340 350
AGLLETDIEP GSTVVCLISG GNNDVLRYGE VLERSLIHLG LKHYFLVDFP
360 370 380 390 400
QKPGALRRFL DEVLGPNDDI TLFEYVKRNN RETGEALVGI QLGSAVDLDV
410 420
LLARMQGTEM HVETLQPGSP AYRYLLL
Length:427
Mass (Da):45,172
Last modified:November 1, 1999 - v1
Checksum:i86FB44221369682E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049478 Genomic DNA Translation: CAB39589.1
AL583921 Genomic DNA Translation: CAC31590.1
PIRiC87060
RefSeqiNP_301876.1, NC_002677.1
WP_010908197.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC31590; CAC31590; CAC31590
GeneIDi910312
KEGGimle:ML1209
PATRICifig|272631.5.peg.2219

Similar proteinsi

Entry informationi

Entry nameiILVA_MYCLE
AccessioniPrimary (citable) accession number: Q9X7F1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 101 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health