Reviewed, UniProtKB/Swiss-Prot Q9X7E6 (ASPG_MYCLE)

Names and origin

Protein names

Recommended name:
    Probable L-asparaginase
      Short name=L-ASNase
    EC=3.5.1.1
Alternative name(s):
    L-asparagine amidohydrolase

Gene names

Name:	ansA
Ordered Locus Names:	ML1198
ORF Names:	MLCB458.13c

Organism

Mycobacterium leprae [Complete proteome] [HAMAP]

Taxonomic identifier

1769 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Probable L-asparaginase		PRO_0000171083
Sites
	Active site	12	1	By similarity
	Active site	82	1	By similarity
	Active site	83	1	By similarity
	Active site	155	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X7E6-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 09471CFF3B781727	FASTA	310	31,798
	10 20 30 40 50 60 MSRLTVIATG GTISTSTDHD GVRRPAYSGT ELTAHVAVDV DVVDLMTVDS SQLIPTDWDR 70 80 90 100 110 120 IRAAVHTATR NGARGVVVTH GTDTMEETAL WLDLTYAGNV PVVLTGAMRS ADAPNADGPT 130 140 150 160 170 180 NLREALAVAA SPAARGVGVL VSFAGRVLQP LGLRKAATQD LSGFAGELLG TSSSGFALTA 190 200 210 220 230 240 AKTRPYLGDL CAADAPRVDI VAAYLGSDSV ALDAYVSTGA RGIVLEALGS GNAGAAVVDG 250 260 270 280 290 300 VRRHCHNGIV IAVSTRVPEG HVSAGYGPGH DLVEAGAVLV PWLRPSQVRV LLMAALAANL 310 PIADVIERWG

References

[1]

"Massive gene decay in the leprosy bacillus." Cole S.T., Eiglmeier K., Parkhill J., James K.D., Thomson N.R., Wheeler P.R., Honore N., Garnier T., Churcher C.M., Harris D.E., Mungall K.L., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K., Duthoy S. , Feltwell T., Fraser A., Hamlin N., Holroyd S., Hornsby T., Jagels K., Lacroix C., Maclean J., Moule S., Murphy L.D., Oliver K., Quail M.A., Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Simon S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Taylor K., Whitehead S., Woodward J.R., Barrell B.G. Nature 409:1007-1011(2001) [PubMed: 11234002] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TN.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL049478 Genomic DNA. Translation: CAB39578.1. AL583921 Genomic DNA. Translation: CAC31579.1.
PIR	H87058.
RefSeq	NP_301872.1.
3D structure databases
HSSP	HSSP built from PDB template 1NNS based on UniProtKB P00805.
ModBase	Search...
Genome annotation databases
GeneID	910300.
GenomeReviews	Gene locus ML1198 in contig AL450380_GR.
KEGG	mle:ML1198.
NMPDR	fig|272631.1.peg.744.
Organism-specific databases
Leproma	ML1198.
CMR	Search...
Phylogenomic databases
HOGENOM	Q9X7E6.
OMA	THTANVE.
Enzyme and pathway databases
BioCyc	MLEP272631:ML1198-MON.
BRENDA	3.5.1.1. 808.
Family and domain databases
InterPro	IPR006034. Asp/Glutamnse. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PRINTS	PR00139. ASNGLNASE.
ProDom	PD003221. Asp/Glutamnse. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_MYCLE

Accession

Primary (citable) accession number: Q9X7E6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 3, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)