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Q9X7E5

- SYI_MYCLE

UniProt

Q9X7E5 - SYI_MYCLE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Zinc.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei640 – 6401ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:ML1195
    ORF Names:MLCB458.10
    OrganismiMycobacterium leprae (strain TN)
    Taxonomic identifieri272631 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000806: Chromosome

    Organism-specific databases

    LepromaiML1195.

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10591059Isoleucine--tRNA ligasePRO_0000098548Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi272631.ML1195.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9X7E5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi637 – 6415"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246403.
    KOiK01870.
    OMAiRVEHMVE.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02003. Ile_tRNA_synth_type2.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X7E5-1 [UniParc]FASTAAdd to Basket

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    MTNSAYPRTD LGADQRGGSP NFPALEAQVL DYWSNDDTFR ASIARCDDAP     50
    EYVFYDGPPF ANGLPHYGHL LTGYVKDIVP RYRTMCGYKV ERRFGWDTHG 100
    LPAELEVERQ LGITDKSQID AMGIAAFNEA CRKSVLRYTE EWQAYVTRQA 150
    RWVDFDNDYK TLDLSYMESV IWAFKQLWDK GLAYESYRVL PYCWRDETPL 200
    SNHELRMDDD VYQSRQDPAV TLGFKVLGGD DEDLVGAYLL VWTTTPWTLP 250
    SNLAVAVHPD VTYVDVRAGD RRFVLAQARL TAYARELGDE PEVLATYRGA 300
    DLLGRHYLPP FQYFYESARN AFQVLPGDFV TTDDGTGIVH IAPAYGEDDM 350
    ATADKVGIVP VTPVDSNGCF DATVPDYQGQ HVFEANAQII RDLKNQSGSA 400
    AVNGAVLLRH ETYEHPYPHC WRCRNPLIYR AVSSWFVAVT EFRDRMVELN 450
    QQITWYPEHV KDGQFGKWLQ GARDWSISRN RYWGTPIPVW KSDDPDYPRI 500
    DVYGSLDELE RDFGVRPTNL HRPYIDELTR PNPDDPTGLS TMRRIPDVFD 550
    VWFDSGSMPY AQVHYPFEND DWFDGFDSAD SDKQVDAHYP GDFIVEYIGQ 600
    ARGWFYTLHV LATALFDRPA FKTCIAHGVV LGSNGQKMSK SLRNYPDVTE 650
    IFDRDGSDAM RWFLMASPIL RGGNLIITEP GIREGMRQVL LPLWNAYSFL 700
    ALYAPKIGTW HTDVSHVLDR YILAKLAVLR DDLSQAMEVC DISGACEQLR 750
    QFTEPLTNWY LRRSRARFWD EDVDAIDTLH TVLEVTARLA APLLPLITEI 800
    IWRSVTGGRS VHLTDWPQAN QLPADPDLVA VMDQVRQVCS AASSLRKAKK 850
    LRVRLPLPKL VVAVYNSQRL KPYIDLIGDE LNVKQIELTD AIDTYGRFEF 900
    TVNARVAGPR LGRDVQAAIK VVKAGEGVAN PDGTLTAGPV VLQPDEYSSR 950
    LVAANPEFTA ELPDGSGLVV LDDTVTPELE AEGWAKDRIR ELQELRKLIG 1000
    LDVSDRIRVL MSVPAERADW ARVHRDFIAR EILATSFEFG EPADSVAIGD 1050
    GVRVSLLKV 1059
    Length:1,059
    Mass (Da):119,809
    Last modified:November 1, 1999 - v1
    Checksum:i67FC7659E9399E39
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049478 Genomic DNA. Translation: CAB39575.1.
    AL583921 Genomic DNA. Translation: CAC31576.1.
    PIRiE87058.
    RefSeqiNP_301871.1. NC_002677.1.
    WP_010908192.1. NC_002677.1.

    Genome annotation databases

    EnsemblBacteriaiCAC31576; CAC31576; CAC31576.
    GeneIDi910297.
    KEGGimle:ML1195.
    PATRICi18054508. VBIMycLep78757_2194.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL049478 Genomic DNA. Translation: CAB39575.1 .
    AL583921 Genomic DNA. Translation: CAC31576.1 .
    PIRi E87058.
    RefSeqi NP_301871.1. NC_002677.1.
    WP_010908192.1. NC_002677.1.

    3D structure databases

    ProteinModelPortali Q9X7E5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 272631.ML1195.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAC31576 ; CAC31576 ; CAC31576 .
    GeneIDi 910297.
    KEGGi mle:ML1195.
    PATRICi 18054508. VBIMycLep78757_2194.

    Organism-specific databases

    Lepromai ML1195.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246403.
    KOi K01870.
    OMAi RVEHMVE.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02003. Ile_tRNA_synth_type2.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023586. Ile-tRNA-ligase_type2.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: TN.

    Entry informationi

    Entry nameiSYI_MYCLE
    AccessioniPrimary (citable) accession number: Q9X7E5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3