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Q9X7E5

- SYI_MYCLE

UniProt

Q9X7E5 - SYI_MYCLE

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei640 – 6401ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:ML1195
ORF Names:MLCB458.10
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
ProteomesiUP000000806: Chromosome

Organism-specific databases

LepromaiML1195.

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10591059Isoleucine--tRNA ligasePRO_0000098548Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML1195.

Structurei

3D structure databases

ProteinModelPortaliQ9X7E5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi637 – 6415"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246403.
KOiK01870.
OMAiRVEHMVE.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X7E5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTNSAYPRTD LGADQRGGSP NFPALEAQVL DYWSNDDTFR ASIARCDDAP
60 70 80 90 100
EYVFYDGPPF ANGLPHYGHL LTGYVKDIVP RYRTMCGYKV ERRFGWDTHG
110 120 130 140 150
LPAELEVERQ LGITDKSQID AMGIAAFNEA CRKSVLRYTE EWQAYVTRQA
160 170 180 190 200
RWVDFDNDYK TLDLSYMESV IWAFKQLWDK GLAYESYRVL PYCWRDETPL
210 220 230 240 250
SNHELRMDDD VYQSRQDPAV TLGFKVLGGD DEDLVGAYLL VWTTTPWTLP
260 270 280 290 300
SNLAVAVHPD VTYVDVRAGD RRFVLAQARL TAYARELGDE PEVLATYRGA
310 320 330 340 350
DLLGRHYLPP FQYFYESARN AFQVLPGDFV TTDDGTGIVH IAPAYGEDDM
360 370 380 390 400
ATADKVGIVP VTPVDSNGCF DATVPDYQGQ HVFEANAQII RDLKNQSGSA
410 420 430 440 450
AVNGAVLLRH ETYEHPYPHC WRCRNPLIYR AVSSWFVAVT EFRDRMVELN
460 470 480 490 500
QQITWYPEHV KDGQFGKWLQ GARDWSISRN RYWGTPIPVW KSDDPDYPRI
510 520 530 540 550
DVYGSLDELE RDFGVRPTNL HRPYIDELTR PNPDDPTGLS TMRRIPDVFD
560 570 580 590 600
VWFDSGSMPY AQVHYPFEND DWFDGFDSAD SDKQVDAHYP GDFIVEYIGQ
610 620 630 640 650
ARGWFYTLHV LATALFDRPA FKTCIAHGVV LGSNGQKMSK SLRNYPDVTE
660 670 680 690 700
IFDRDGSDAM RWFLMASPIL RGGNLIITEP GIREGMRQVL LPLWNAYSFL
710 720 730 740 750
ALYAPKIGTW HTDVSHVLDR YILAKLAVLR DDLSQAMEVC DISGACEQLR
760 770 780 790 800
QFTEPLTNWY LRRSRARFWD EDVDAIDTLH TVLEVTARLA APLLPLITEI
810 820 830 840 850
IWRSVTGGRS VHLTDWPQAN QLPADPDLVA VMDQVRQVCS AASSLRKAKK
860 870 880 890 900
LRVRLPLPKL VVAVYNSQRL KPYIDLIGDE LNVKQIELTD AIDTYGRFEF
910 920 930 940 950
TVNARVAGPR LGRDVQAAIK VVKAGEGVAN PDGTLTAGPV VLQPDEYSSR
960 970 980 990 1000
LVAANPEFTA ELPDGSGLVV LDDTVTPELE AEGWAKDRIR ELQELRKLIG
1010 1020 1030 1040 1050
LDVSDRIRVL MSVPAERADW ARVHRDFIAR EILATSFEFG EPADSVAIGD

GVRVSLLKV
Length:1,059
Mass (Da):119,809
Last modified:November 1, 1999 - v1
Checksum:i67FC7659E9399E39
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049478 Genomic DNA. Translation: CAB39575.1.
AL583921 Genomic DNA. Translation: CAC31576.1.
PIRiE87058.
RefSeqiNP_301871.1. NC_002677.1.
WP_010908192.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC31576; CAC31576; CAC31576.
GeneIDi910297.
KEGGimle:ML1195.
PATRICi18054508. VBIMycLep78757_2194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL049478 Genomic DNA. Translation: CAB39575.1 .
AL583921 Genomic DNA. Translation: CAC31576.1 .
PIRi E87058.
RefSeqi NP_301871.1. NC_002677.1.
WP_010908192.1. NC_002677.1.

3D structure databases

ProteinModelPortali Q9X7E5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 272631.ML1195.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAC31576 ; CAC31576 ; CAC31576 .
GeneIDi 910297.
KEGGi mle:ML1195.
PATRICi 18054508. VBIMycLep78757_2194.

Organism-specific databases

Lepromai ML1195.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246403.
KOi K01870.
OMAi RVEHMVE.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiSYI_MYCLE
AccessioniPrimary (citable) accession number: Q9X7E5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3