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Protein

DNA-directed RNA polymerase subunit alpha

Gene

rpoA

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.UniRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-2151-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase subunit alphaUniRule annotation (EC:2.7.7.6UniRule annotation)
Short name:
RNAP subunit alphaUniRule annotation
Alternative name(s):
RNA polymerase subunit alphaUniRule annotation
Transcriptase subunit alphaUniRule annotation
Gene namesi
Name:rpoAUniRule annotation
Ordered Locus Names:ML1957
ORF Names:MLCB1222.27c
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML1957

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001753371 – 347DNA-directed RNA polymerase subunit alphaAdd BLAST347

Proteomic databases

PRIDEiQ9X798

Interactioni

Subunit structurei

Homodimer. The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi272631.ML1957

Structurei

3D structure databases

ProteinModelPortaliQ9X798
SMRiQ9X798
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 226Alpha N-terminal domain (alpha-NTD)UniRule annotationAdd BLAST226
Regioni243 – 347Alpha C-terminal domain (alpha-CTD)UniRule annotationAdd BLAST105

Domaini

The N-terminal domain is essential for RNAP assembly and basal transcription, whereas the C-terminal domain is involved in interaction with transcriptional regulators and with upstream promoter elements.UniRule annotation

Sequence similaritiesi

Belongs to the RNA polymerase alpha chain family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTF Bacteria
COG0202 LUCA
HOGENOMiHOG000218480
KOiK03040
OMAiLMKFRNF
OrthoDBiPOG091H001Z

Family and domain databases

Gene3Di2.170.120.12, 1 hit
3.30.1360.10, 2 hits
HAMAPiMF_00059 RNApol_bact_RpoA, 1 hit
InterProiView protein in InterPro
IPR011262 DNA-dir_RNA_pol_insert
IPR011263 DNA-dir_RNA_pol_RpoA/D/Rpb3
IPR011773 DNA-dir_RpoA
IPR036603 RBP11-like
IPR011260 RNAP_asu_C
IPR036643 RNApol_insert_sf
PANTHERiPTHR32108 PTHR32108, 1 hit
PfamiView protein in Pfam
PF01000 RNA_pol_A_bac, 1 hit
PF03118 RNA_pol_A_CTD, 1 hit
PF01193 RNA_pol_L, 1 hit
ProDomiView protein in ProDom or Entries sharing at least one domain
PD001179 RNAP_asu_C, 1 hit
SMARTiView protein in SMART
SM00662 RPOLD, 1 hit
SUPFAMiSSF55257 SSF55257, 2 hits
SSF56553 SSF56553, 1 hit
TIGRFAMsiTIGR02027 rpoA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9X798-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLISQRPTLS EDILTDNRSQ FVIEPLEPGF GYTLGNSLRR TLLSSIPGAA
60 70 80 90 100
VTSIRIDGVL HEFTTVPGVK EDVTEIILNL KGLVVSSEED EPVTMYLRKQ
110 120 130 140 150
GPGEVTAGDI VPPAGVTLHN PGMRIATLND KGKIEAELVV ERGRGYVPAV
160 170 180 190 200
QNRALGAEIG RIPVDSIYSP VLKVTYKVDA TRVEQRTDFD KLILDVETKS
210 220 230 240 250
SITPRDALAS AGKTLVELFG LARELNVEAE GIEIGPSPAE ADHIASFALP
260 270 280 290 300
IDDLDLTVRS YNCLKREGVH TVGELVSRTE SDLLDIRNFG QKSIDEVKVK
310 320 330 340
LHQLGLSLKD SPDSFDPSEV AGYDVTTGTW STDGAYDSQD YAETEQL
Length:347
Mass (Da):37,702
Last modified:November 1, 1999 - v1
Checksum:iAC61A7BBA2FBA994
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL049491 Genomic DNA Translation: CAB39833.1
AL583923 Genomic DNA Translation: CAC30912.1
PIRiH87153
RefSeqiNP_302322.1, NC_002677.1
WP_010908643.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC30912; CAC30912; CAC30912
GeneIDi909427
KEGGimle:ML1957
PATRICifig|272631.5.peg.3707

Similar proteinsi

Entry informationi

Entry nameiRPOA_MYCLE
AccessioniPrimary (citable) accession number: Q9X798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: May 23, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health