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Q9X721 (Q9X721_CLOHI) Unreviewed, UniProtKB/TrEMBL

Last modified January 22, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
Gene names
Name:colG EMBL BAA77453.1
OrganismClostridium histolyticum EMBL BAA77453.1
Taxonomic identifier1498 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length1118 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding5231Zinc; via tele nitrogen PDB 2Y50 PDB 2Y6I PDB 4ARE
Metal binding5271Zinc; via tele nitrogen PDB 2Y50 PDB 2Y6I PDB 4ARE
Metal binding5551Zinc PDB 2Y50 PDB 2Y6I PDB 4ARE
Metal binding7951Calcium 1 PDB 4AQO
Metal binding7961Calcium 1; via carbonyl oxygen PDB 4AQO
Metal binding8231Calcium 1 PDB 4AQO
Metal binding8251Calcium 1 PDB 4AQO
Metal binding8641Calcium 1 PDB 4AQO
Metal binding10091Calcium 2 PDB 4HPK
Metal binding10111Calcium 2 PDB 4HPK
Metal binding10111Calcium 3 PDB 4HPK
Metal binding10131Calcium 3 PDB 4HPK
Metal binding10141Calcium 3 PDB 4HPK
Metal binding10321Calcium 2; via carbonyl oxygen PDB 4HPK
Metal binding10371Calcium 2 PDB 4HPK
Metal binding10371Calcium 3 PDB 4HPK
Metal binding10391Calcium 3; via carbonyl oxygen PDB 4HPK
Metal binding10401Calcium 2 PDB 4HPK
Metal binding10401Calcium 3 PDB 4HPK
Binding site2781Hexaethylene glycol 3; via carbonyl oxygen PDB 4ARE
Binding site3371Hexaethylene glycol 3 PDB 4ARE
Binding site3441Hexaethylene glycol 3 PDB 4ARE
Binding site5681Citrate 1; via carbonyl oxygen PDB 2Y6I
Binding site6181Citrate 1 PDB 2Y6I
Binding site6181Citrate 2 PDB 4ARE
Binding site6181Citrate 3 PDB 2Y3U PDB 2Y50
Binding site7371Citrate 1 PDB 2Y6I
Binding site7371Citrate 2 PDB 4ARE
Binding site7371Citrate 3 PDB 2Y3U PDB 2Y50
Binding site10471Chloride; via amide nitrogen PDB 4HPK

Sequences

Sequence LengthMass (Da)Tools
Q9X721 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 770CF3F60E4D4FD4

FASTA1,118126,242
        10         20         30         40         50         60 
MKKNILKILM DSYSKESKIQ TVRRVTSVSL LAVYLTMNTS SLVLAKPIEN TNDTSIKNVE 

        70         80         90        100        110        120 
KLRNAPNEEN SKKVEDSKND KVEHVKNIEE AKVEQVAPEV KSKSTLRSAS IANTNSEKYD 

       130        140        150        160        170        180 
FEYLNGLSYT ELTNLIKNIK WNQINGLFNY STGSQKFFGD KNRVQAIINA LQESGRTYTA 

       190        200        210        220        230        240 
NDMKGIETFT EVLRAGFYLG YYNDGLSYLN DRNFQDKCIP AMIAIQKNPN FKLGTAVQDE 

       250        260        270        280        290        300 
VITSLGKLIG NASANAEVVN NCVPVLKQFR ENLNQYAPDY VKGTAVNELI KGIEFDFSGA 

       310        320        330        340        350        360 
AYEKDVKTMP WYGKIDPFIN ELKALGLYGN ITSATEWASD VGIYYLSKFG LYSTNRNDIV 

       370        380        390        400        410        420 
QSLEKAVDMY KYGKIAFVAM ERITWDYDGI GSNGKKVDHD KFLDDAEKHY LPKTYTFDNG 

       430        440        450        460        470        480 
TFIIRAGDKV SEEKIKRLYW ASREVKSQFH RVVGNDKALE VGNADDVLTM KIFNSPEEYK 

       490        500        510        520        530        540 
FNTNINGVST DNGGLYIEPR GTFYTYERTP QQSIFSLEEL FRHEYTHYLQ ARYLVDGLWG 

       550        560        570        580        590        600 
QGPFYEKNRL TWFDEGTAEF FAGSTRTSGV LPRKSILGYL AKDKVDHRYS LKKTLNSGYD 

       610        620        630        640        650        660 
DSDWMFYNYG FAVAHYLYEK DMPTFIKMNK AILNTDVKSY DEIIKKLSDD ANKNTEYQNH 

       670        680        690        700        710        720 
IQELADKYQG AGIPLVSDDY LKDHGYKKAS EVYSEISKAA SLTNTSVTAE KSQYFNTFTL 

       730        740        750        760        770        780 
RGTYTGETSK GEFKDWDEMS KKLDGTLESL AKNSWSGYKT LTAYFTNYRV TSDNKVQYDV 

       790        800        810        820        830        840 
VFHGVLTDNA DISNNKAPIA KVTGPSTGAV GRNIEFSGKD SKDEDGKIVS YDWDFGDGAT 

       850        860        870        880        890        900 
SRGKNSVHAY KKAGTYNVTL KVTDDKGATA TESFTIEIKN EDTTTPITKE MEPNDDIKEA 

       910        920        930        940        950        960 
NGPIVEGVTV KGDLNGSDDA DTFYFDVKED GDVTIELPYS GSSNFTWLVY KEGDDQNHIA 

       970        980        990       1000       1010       1020 
SGIDKNNSKV GTFKSTKGRH YVFIYKHDSA SNISYSLNIK GLGNEKLKEK ENNDSSDKAT 

      1030       1040       1050       1060       1070       1080 
VIPNFNTTMQ GSLLGDDSRD YYSFEVKEEG EVNIELDKKD EFGVTWTLHP ESNINDRITY 

      1090       1100       1110 
GQVDGNKVSN KVKLRPGKYY LLVYKYSGSG NYELRVNK 

« Hide

References

[1]"Gene duplication and multiplicity of collagenases in Clostridium histolyticum."
Matsushita O., Jung C.-M., Katayama S., Minami J., Takahashi Y., Okabe A.
J. Bacteriol. 181:923-933(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: JCM 1403 EMBL BAA77453.1.
[2]"Crystal structure of Clostridium histolyticum colg collagenase PKD domain 2 at 1.6 Angstrom resolution."
Sakon J., Philominathan S.T.L., Gunn S., Matsushita O.
Submitted (SEP-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS) OF 797-881.
[3]"Crystal structure of Clostridium histolyticum colG collagenase polycystic kidney disease domain at 1.4 Angstrom resolution."
Sakon J, Philominathan S.T.L., Matsushita O., Gunn S.
Submitted (SEP-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.40 ANGSTROMS) OF 797-881.
[4]"Structure of collagenase G reveals a chew-and-digest mechanism of bacterial collagenolysis."
Eckhard U., Schonauer E., Nuss D., Brandstetter H.
Nat. Struct. Mol. Biol. 18:1109-1114(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.18 ANGSTROMS) OF 799-880 IN COMPLEX WITH CITRATE AND ZINC.
[5]"Structural comparison of ColH and ColG collagen-binding domains from Clostridium histolyticum."
Bauer R., Wilson J.J., Philominathan S.T., Davis D., Matsushita O., Sakon J.
J. Bacteriol. 195:318-327(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.35 ANGSTROMS) OF 1006-1118 AND 1002-1118 IN COMPLEX WITH CALCIUM AND CHLORIDE.
[6]"Structural basis for activity regulation and substrate preference of clostridial collagenases G, H, and T."
Eckhard U., Schonauer E., Brandstetter H.
J. Biol. Chem. 288:20184-20194(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (0.99 ANGSTROMS) OF 792-880 IN COMPLEX WITH CALCIUM; CITRATE; HEXAETHYLENE GLYCOL AND ZINC.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87215 Genomic DNA. Translation: BAA77453.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Y3UX-ray2.55A119-880[»]
2Y50X-ray2.80A119-880[»]
2Y6IX-ray3.25A119-880[»]
2Y72X-ray1.18A/B799-880[»]
3JQUX-ray1.40A/B797-881[»]
3JS7X-ray1.60A/B797-881[»]
4AQOX-ray0.99A792-880[»]
4AREX-ray2.19A119-790[»]
4HPKX-ray1.35A1006-1118[»]
B1002-1118[»]
ProteinModelPortalQ9X721.
SMRQ9X721. Positions 1005-1118.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome5989. Clo hi Collagenase.
MEROPSM09.002.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.670. 1 hit.
InterProIPR007280. Peptidase_C_arc/bac.
IPR013661. Peptidase_M9_N_dom.
IPR002169. Peptidase_M9A/M9B.
IPR022409. PKD/Chitinase_dom.
IPR000601. PKD_dom.
[Graphical view]
PfamPF01752. Peptidase_M9. 1 hit.
PF08453. Peptidase_M9_N. 1 hit.
PF00801. PKD. 1 hit.
PF04151. PPC. 1 hit.
[Graphical view]
PRINTSPR00931. MICOLLPTASE.
SMARTSM00089. PKD. 1 hit.
[Graphical view]
SUPFAMSSF49299. SSF49299. 1 hit.
PROSITEPS50093. PKD. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceQ9X721.

Entry information

Entry nameQ9X721_CLOHI
AccessionPrimary (citable) accession number: Q9X721
Entry history
Integrated into UniProtKB/TrEMBL: November 1, 1999
Last sequence update: November 1, 1999
Last modified: January 22, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)