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Q9X706

- GLND_CORGL

UniProt

Q9X706 - GLND_CORGL

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism (By similarity).By similarity

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].

Cofactori

Magnesium.By similarity

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.By similarity

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-EC
  2. hydrolase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BRENDAi2.7.7.59. 1648.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Cgl2059, cg2258
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192730Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg2258.

Structurei

3D structure databases

ProteinModelPortaliQ9X706.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini384 – 47491HDAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 270270UridylyltransferaseAdd
BLAST
Regioni271 – 692422Uridylyl-removingAdd
BLAST

Domaini

Has two distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, and a HD domain that encodes UR activity (By similarity). Lacks the two C-terminal ACT domains found in GlnD orthologs, that seem to have a role in glutamine sensing.By similarity

Sequence similaritiesi

Belongs to the GlnD family.Curated
Contains 1 HD domain.Curated

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000021840.
KOiK00990.
OMAiGCALETV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X706-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNNPAQLRQD TEKEVLALLG SLVLPAGTAL AATGSLARSE LTPYSDLDLI
60 70 80 90 100
LIHPPGATPD GVEDLWYPIW DAKKRLDYSV RTPDECVAMI SADSTAALAM
110 120 130 140 150
LDLRFVAGDE DLCAKTRRRI VEKWRQELNK NFDAVVDTAI ARWRRSGPVV
160 170 180 190 200
AMTRPDLKHG RGGLRDFELI KALALGHLCN LPQLDAQHQL LLDARTLLHV
210 220 230 240 250
HARRSRDVLD PEFAVDVAMD LGFVDRYHLG REIADAARAI DDGLTTALAT
260 270 280 290 300
ARGILPRRTG FAFRNASRRP LDLDVVDANG TIELSKKPDL NDPALPLRVA
310 320 330 340 350
AAAATTGLPV AESTWVRLNE CPPLPEPWPA NAAGDFFRIL SSPKNSRRVV
360 370 380 390 400
KNMDRHGLWS RFVPEWDRIK GLMPREPSHI STIDEHSLNT VAGCALETVT
410 420 430 440 450
VARPDLLVLG ALYHDIGKGF PRPHEQVGAE MVARAASRMG LNLRDRASVQ
460 470 480 490 500
TLVAEHTAVA KIAARLDPSS EGAVDKLLDA VRYDLVTLNL LEVLTEADAK
510 520 530 540 550
ATGPGVWTAR LEHALRIVCK RARDRLTDIR PVAPMIAPRS EIGLVERDGV
560 570 580 590 600
FTVQWHGEDL HRILGVIYAK GWTITAARML ANGQWSAEFD VRANGPQDFD
610 620 630 640 650
PQHFLQAYQS GVFSEVPIPA LGITATFWHG NTLEVRTELR TGAIFALLRT
660 670 680 690
LPDALWINAV TRGATLIIQA ALKPGFDRAT VERSVVRSLA GS
Length:692
Mass (Da):75,970
Last modified:November 1, 1999 - v1
Checksum:iA7AC19614C133676
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010319 Genomic DNA. Translation: CAB39374.1.
BA000036 Genomic DNA. Translation: BAB99452.1.
BX927154 Genomic DNA. Translation: CAF20397.1.
RefSeqiNP_601262.1. NC_003450.3.
WP_011014852.1. NC_006958.1.
YP_226298.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99452; BAB99452; BAB99452.
CAF20397; CAF20397; cg2258.
GeneIDi1020013.
3343788.
KEGGicgb:cg2258.
cgl:NCgl1981.
PATRICi21496122. VBICorGlu203724_1997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010319 Genomic DNA. Translation: CAB39374.1 .
BA000036 Genomic DNA. Translation: BAB99452.1 .
BX927154 Genomic DNA. Translation: CAF20397.1 .
RefSeqi NP_601262.1. NC_003450.3.
WP_011014852.1. NC_006958.1.
YP_226298.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q9X706.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2258.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99452 ; BAB99452 ; BAB99452 .
CAF20397 ; CAF20397 ; cg2258 .
GeneIDi 1020013.
3343788.
KEGGi cgb:cg2258.
cgl:NCgl1981.
PATRICi 21496122. VBICorGlu203724_1997.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000021840.
KOi K00990.
OMAi GCALETV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BRENDAi 2.7.7.59. 1648.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins."
    Jakoby M.J., Kraemer R., Burkovski A.
    FEMS Microbiol. Lett. 173:303-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiGLND_CORGL
AccessioniPrimary (citable) accession number: Q9X706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3