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Q9X706

- GLND_CORGL

UniProt

Q9X706 - GLND_CORGL

Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Nov 1999)
      Previous versions | rss
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    Functioni

    Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.By similarity

    Catalytic activityi

    UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].
    Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.By similarity

    GO - Molecular functioni

    1. [protein-PII] uridylyltransferase activity Source: UniProtKB-EC
    2. metal ion binding Source: InterPro
    3. phosphoric diester hydrolase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium

    Enzyme and pathway databases

    BRENDAi2.7.7.59. 1648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional uridylyltransferase/uridylyl-removing enzyme
    Short name:
    UTase/UR
    Alternative name(s):
    Bifunctional [protein-PII] modification enzyme
    Bifunctional nitrogen sensor protein
    Including the following 2 domains:
    [Protein-PII] uridylyltransferase (EC:2.7.7.59)
    Short name:
    PII uridylyltransferase
    Short name:
    UTase
    [Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
    Short name:
    UR
    Gene namesi
    Name:glnD
    Ordered Locus Names:Cgl2059, cg2258
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_0000192730Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg2258.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9X706.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini384 – 47491HDAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 270270UridylyltransferaseAdd
    BLAST
    Regioni271 – 692422Uridylyl-removingAdd
    BLAST

    Domaini

    Has two distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, and a HD domain that encodes UR activity By similarity. Lacks the two C-terminal ACT domains found in GlnD orthologs, that seem to have a role in glutamine sensing.By similarity

    Sequence similaritiesi

    Belongs to the GlnD family.Curated
    Contains 1 HD domain.Curated

    Phylogenomic databases

    eggNOGiCOG2844.
    HOGENOMiHOG000021840.
    KOiK00990.
    OMAiGCALETV.
    OrthoDBiEOG6CCH44.

    Family and domain databases

    Gene3Di1.10.3210.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view]
    PfamiPF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view]
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X706-1 [UniParc]FASTAAdd to Basket

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    MNNPAQLRQD TEKEVLALLG SLVLPAGTAL AATGSLARSE LTPYSDLDLI    50
    LIHPPGATPD GVEDLWYPIW DAKKRLDYSV RTPDECVAMI SADSTAALAM 100
    LDLRFVAGDE DLCAKTRRRI VEKWRQELNK NFDAVVDTAI ARWRRSGPVV 150
    AMTRPDLKHG RGGLRDFELI KALALGHLCN LPQLDAQHQL LLDARTLLHV 200
    HARRSRDVLD PEFAVDVAMD LGFVDRYHLG REIADAARAI DDGLTTALAT 250
    ARGILPRRTG FAFRNASRRP LDLDVVDANG TIELSKKPDL NDPALPLRVA 300
    AAAATTGLPV AESTWVRLNE CPPLPEPWPA NAAGDFFRIL SSPKNSRRVV 350
    KNMDRHGLWS RFVPEWDRIK GLMPREPSHI STIDEHSLNT VAGCALETVT 400
    VARPDLLVLG ALYHDIGKGF PRPHEQVGAE MVARAASRMG LNLRDRASVQ 450
    TLVAEHTAVA KIAARLDPSS EGAVDKLLDA VRYDLVTLNL LEVLTEADAK 500
    ATGPGVWTAR LEHALRIVCK RARDRLTDIR PVAPMIAPRS EIGLVERDGV 550
    FTVQWHGEDL HRILGVIYAK GWTITAARML ANGQWSAEFD VRANGPQDFD 600
    PQHFLQAYQS GVFSEVPIPA LGITATFWHG NTLEVRTELR TGAIFALLRT 650
    LPDALWINAV TRGATLIIQA ALKPGFDRAT VERSVVRSLA GS 692
    Length:692
    Mass (Da):75,970
    Last modified:November 1, 1999 - v1
    Checksum:iA7AC19614C133676
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010319 Genomic DNA. Translation: CAB39374.1.
    BA000036 Genomic DNA. Translation: BAB99452.1.
    BX927154 Genomic DNA. Translation: CAF20397.1.
    RefSeqiNP_601262.1. NC_003450.3.
    WP_011014852.1. NC_006958.1.
    YP_226298.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB99452; BAB99452; BAB99452.
    CAF20397; CAF20397; cg2258.
    GeneIDi1020013.
    KEGGicgb:cg2258.
    cgl:NCgl1981.
    PATRICi21496122. VBICorGlu203724_1997.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ010319 Genomic DNA. Translation: CAB39374.1 .
    BA000036 Genomic DNA. Translation: BAB99452.1 .
    BX927154 Genomic DNA. Translation: CAF20397.1 .
    RefSeqi NP_601262.1. NC_003450.3.
    WP_011014852.1. NC_006958.1.
    YP_226298.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali Q9X706.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg2258.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB99452 ; BAB99452 ; BAB99452 .
    CAF20397 ; CAF20397 ; cg2258 .
    GeneIDi 1020013.
    KEGGi cgb:cg2258.
    cgl:NCgl1981.
    PATRICi 21496122. VBICorGlu203724_1997.

    Phylogenomic databases

    eggNOGi COG2844.
    HOGENOMi HOG000021840.
    KOi K00990.
    OMAi GCALETV.
    OrthoDBi EOG6CCH44.

    Enzyme and pathway databases

    BRENDAi 2.7.7.59. 1648.

    Family and domain databases

    Gene3Di 1.10.3210.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR006674. HD_domain.
    IPR002934. Nucleotidyltransferase.
    IPR013546. PII_UdlTrfase/GS_AdlTrfase.
    [Graphical view ]
    Pfami PF08335. GlnD_UR_UTase. 1 hit.
    PF01966. HD. 1 hit.
    PF01909. NTP_transf_2. 1 hit.
    [Graphical view ]
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins."
      Jakoby M.J., Kraemer R., Burkovski A.
      FEMS Microbiol. Lett. 173:303-310(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiGLND_CORGL
    AccessioniPrimary (citable) accession number: Q9X706
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: November 1, 1999
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3