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Q9X706

- GLND_CORGL

UniProt

Q9X706 - GLND_CORGL

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, Cgl2059, cg2258
Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].

Cofactori

Magnesium By similarity.

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-EC
  2. metal ion binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BRENDAi2.7.7.59. 1648.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:Cgl2059, cg2258
OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Taxonomic identifieri196627 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692Bifunctional uridylyltransferase/uridylyl-removing enzyme
PRO_0000192730Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi196627.cg2258.

Structurei

3D structure databases

ProteinModelPortaliQ9X706.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini384 – 47491HD
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 270270Uridylyltransferase
Add
BLAST
Regioni271 – 692422Uridylyl-removing
Add
BLAST

Domaini

Has two distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, and a HD domain that encodes UR activity By similarity. Lacks the two C-terminal ACT domains found in GlnD orthologs, that seem to have a role in glutamine sensing.

Sequence similaritiesi

Belongs to the GlnD family.
Contains 1 HD domain.

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000021840.
KOiK00990.
OMAiGCALETV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
SMARTiSM00471. HDc. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X706-1 [UniParc]FASTAAdd to Basket

« Hide

MNNPAQLRQD TEKEVLALLG SLVLPAGTAL AATGSLARSE LTPYSDLDLI    50
LIHPPGATPD GVEDLWYPIW DAKKRLDYSV RTPDECVAMI SADSTAALAM 100
LDLRFVAGDE DLCAKTRRRI VEKWRQELNK NFDAVVDTAI ARWRRSGPVV 150
AMTRPDLKHG RGGLRDFELI KALALGHLCN LPQLDAQHQL LLDARTLLHV 200
HARRSRDVLD PEFAVDVAMD LGFVDRYHLG REIADAARAI DDGLTTALAT 250
ARGILPRRTG FAFRNASRRP LDLDVVDANG TIELSKKPDL NDPALPLRVA 300
AAAATTGLPV AESTWVRLNE CPPLPEPWPA NAAGDFFRIL SSPKNSRRVV 350
KNMDRHGLWS RFVPEWDRIK GLMPREPSHI STIDEHSLNT VAGCALETVT 400
VARPDLLVLG ALYHDIGKGF PRPHEQVGAE MVARAASRMG LNLRDRASVQ 450
TLVAEHTAVA KIAARLDPSS EGAVDKLLDA VRYDLVTLNL LEVLTEADAK 500
ATGPGVWTAR LEHALRIVCK RARDRLTDIR PVAPMIAPRS EIGLVERDGV 550
FTVQWHGEDL HRILGVIYAK GWTITAARML ANGQWSAEFD VRANGPQDFD 600
PQHFLQAYQS GVFSEVPIPA LGITATFWHG NTLEVRTELR TGAIFALLRT 650
LPDALWINAV TRGATLIIQA ALKPGFDRAT VERSVVRSLA GS 692
Length:692
Mass (Da):75,970
Last modified:November 1, 1999 - v1
Checksum:iA7AC19614C133676
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010319 Genomic DNA. Translation: CAB39374.1.
BA000036 Genomic DNA. Translation: BAB99452.1.
BX927154 Genomic DNA. Translation: CAF20397.1.
RefSeqiNP_601262.1. NC_003450.3.
WP_011014852.1. NC_006958.1.
YP_226298.1. NC_006958.1.

Genome annotation databases

EnsemblBacteriaiBAB99452; BAB99452; BAB99452.
CAF20397; CAF20397; cg2258.
GeneIDi1020013.
KEGGicgb:cg2258.
cgl:NCgl1981.
PATRICi21496122. VBICorGlu203724_1997.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ010319 Genomic DNA. Translation: CAB39374.1 .
BA000036 Genomic DNA. Translation: BAB99452.1 .
BX927154 Genomic DNA. Translation: CAF20397.1 .
RefSeqi NP_601262.1. NC_003450.3.
WP_011014852.1. NC_006958.1.
YP_226298.1. NC_006958.1.

3D structure databases

ProteinModelPortali Q9X706.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196627.cg2258.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAB99452 ; BAB99452 ; BAB99452 .
CAF20397 ; CAF20397 ; cg2258 .
GeneIDi 1020013.
KEGGi cgb:cg2258.
cgl:NCgl1981.
PATRICi 21496122. VBICorGlu203724_1997.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000021840.
KOi K00990.
OMAi GCALETV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BRENDAi 2.7.7.59. 1648.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nitrogen regulation in Corynebacterium glutamicum: isolation of genes involved and biochemical characterization of corresponding proteins."
    Jakoby M.J., Kraemer R., Burkovski A.
    FEMS Microbiol. Lett. 173:303-310(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  2. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
    Ikeda M., Nakagawa S.
    Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Entry informationi

Entry nameiGLND_CORGL
AccessioniPrimary (citable) accession number: Q9X706
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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