Q9X6Y8 (MURB_BORPE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	BP2510

Organism

Bordetella pertussis

Taxonomic identifier

520 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Alcaligenaceae › Bordetella

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000179183
Regions
	Domain	29 – 199	171	FAD-binding PCMH-type
Sites
	Active site	175	1	By similarity
	Active site	248	1	Proton donor By similarity
	Active site	344	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X6Y8 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 81E10E9A148B70F2	FASTA	351	37,364
	10 20 30 40 50 60 MSTVPARIEP VAPLAPQAQD LRCFNTLGLA SHAPAFVALT EPSQLPALSA LAPRFRQLVV 70 80 90 100 110 120 LGGGSNVVLP ASIDGLVAQV RLPGVRLVGQ CADAWVVEAA AGENWHGFVT ACVDNGWDGL 130 140 150 160 170 180 ENLALIPGTV GAAPVQNIGA YGVELADRFH SLTAWDVKGG RWVEMGAAEC RFAYRDSFFK 190 200 210 220 230 240 HQEPGAWVIG SVRFALPRPW QPVLDYPDLQ RHAALDGAAP TARAVYDAVC AIRRAKLPDP 250 260 270 280 290 300 AVVGNAGSFF KNPLVDAGTR QALLGRFPGL VSYPQPDGRY KLAAGWLIDQ CGWKGRQLGA 310 320 330 340 350 AGVHDRQALV LVNRGGAQAR DIMALAAAIQ GDVERRYGVR LEPEPVVVPA R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Pradel E. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.
[2]	"Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica." Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R., Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L., Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A., Achtman M., Atkin R., Baker S. , Basham D., Bason N., Cherevach I., Chillingworth T., Collins M., Cronin A., Davis P., Doggett J., Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K., Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C., Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K., Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K., Unwin L., Whitehead S., Barrell B.G., Maskell D.J. Nat. Genet. 35:32-40(2003) [PubMed: 12910271] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tohama I / ATCC BAA-589 / NCTC 13251.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ238308 Genomic DNA. Translation: CAB41011.1. BX640418 Genomic DNA. Translation: CAE42782.1.
RefSeq	NP_881137.1. NC_002929.2.
3D structure databases
ProteinModelPortal	Q9X6Y8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	2665671.
GenomeReviews	Gene locus BP2510 in contig BX470248_GR.
KEGG	bpe:BP2510.
NMPDR	fig|257313.1.peg.2211.
PATRIC	21158454. VBIBorPer7866_2708.
Phylogenomic databases
HOGENOM	HBG686573.
OMA	ISERHAN.
PhylomeDB	Q9X6Y8.
ProtClustDB	PRK00046.
Enzyme and pathway databases
BioCyc	BPER257313:BP2510-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_BORPE

Accession

Primary (citable) accession number: Q9X6Y8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program