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Protein

Peptidoglycan D,D-transpeptidase MrdA

Gene

pbpA

Organism
Pseudomonas aeruginosa
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes cross-linking of the peptidoglycan cell wall.UniRule annotation

Catalytic activityi

Preferential cleavage: (Ac)2-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.UniRule annotation

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei327Acyl-ester intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCarboxypeptidaseUniRule annotation, Hydrolase, Protease
Biological processCell shape, Cell wall biogenesis/degradationUniRule annotation, Peptidoglycan synthesisUniRule annotation

Enzyme and pathway databases

UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidoglycan D,D-transpeptidase MrdAUniRule annotation (EC:3.4.16.4UniRule annotation)
Alternative name(s):
Penicillin-binding protein 2UniRule annotation
Short name:
PBP-2UniRule annotation
Gene namesi
Name:pbpAImported
Synonyms:mrdAUniRule annotationImported, spoVDImported
ORF Names:AO964_03845Imported, AOY09_05445Imported, CAZ03_33860Imported, CAZ10_26315Imported, CGU42_07245Imported, CWI21_05040Imported, PAERUG_E15_London_28_01_14_04647Imported, RW109_RW109_01676Imported
OrganismiPseudomonas aeruginosaImported
Taxonomic identifieri287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000194726 Componenti: Unassembled WGS sequence
  • UP000233777 Componenti: Chromosome mainomosome
  • UP000054840 Componenti: Unassembled WGS sequence
  • UP000232017 Componenti: Chromosome
  • UP000216659 Componenti: Unassembled WGS sequence
  • UP000194857 Componenti: Unassembled WGS sequence

Organism-specific databases

PseudoCAPiPA4003.

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 40HelicalUniRule annotationAdd BLAST20

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membraneUniRule annotation, Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5539.
DrugBankiDB01413. Cefepime.
DB00438. Ceftazidime.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 236PBP_dimerInterPro annotationAdd BLAST173
Domaini268 – 604TranspeptidaseInterPro annotationAdd BLAST337

Sequence similaritiesi

Belongs to the transpeptidase family. MrdA subfamily.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helixUniRule annotation

Phylogenomic databases

eggNOGiENOG4105CJN. Bacteria.
COG0768. LUCA.
HOGENOMiHOG000266120.
KOiK05515.

Family and domain databases

HAMAPiMF_02081. MrdA_transpept. 1 hit.
InterProiView protein in InterPro
IPR012338. Beta-lactam/transpept-like.
IPR005311. PBP_dimer.
IPR036138. PBP_dimer_sf.
IPR001460. PCN-bd_Tpept.
IPR017790. Penicillin-binding_protein_2.
PfamiView protein in Pfam
PF03717. PBP_dimer. 1 hit.
PF00905. Transpeptidase. 1 hit.
SUPFAMiSSF56519. SSF56519. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03423. pbp2_mrdA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X6V3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPQPIHLKDH EKDARLVRRR VIVGAVAVVL LTLVLVARMY HLQVTQYEYH
60 70 80 90 100
STLSENNRVH VQPIPPTRGI IFDRNGVIIA DNRPSFSLTI TRERTEDLQK
110 120 130 140 150
TLDTLVEILG LTEDDRAIFE KRMKQGRRPF EPVPIMFELN EEQIARIAVN
160 170 180 190 200
QFRLPGVDVA TQFVRHYPLK EHFAHSVGYV GRINEQELKN LDPINYSGTH
210 220 230 240 250
HIGKTGIERF YESELHGTVG YEEVETNARG RVLRVLKRTD PIPGKDIVLS
260 270 280 290 300
IDSRLQEAAE NALAGRRGAI VAIQPSTGDV LAMVSQPSYD PNLFVTGISF
310 320 330 340 350
KAYAELRDSI DRPLYNRVLR GLYPPGSTVK PAVALAGLDA GVVTPTSRVF
360 370 380 390 400
DPGYYQLPNY DHKYRNWNRY GDGWVSLESA IYRSNDTYFY DLAHKLGIDR
410 420 430 440 450
LHAFMSRFGF GQKVALDMFG EADGLMPSRE WKRKTRRQVW YPGETLILGI
460 470 480 490 500
GQGYMQATPI QLAQMTALLA NKGHWIRPHL AKTIDGQPPV DPDPMPDIVL
510 520 530 540 550
RDPANWDRVD YGMQQVVHGA RGTARKVGAT SAYLIAGKSG TAQVVAIKQN
560 570 580 590 600
ERYDRSKLLE RHRDHALFVG FAPANNPQIA VAVMVENGES GSGVAAPVVK
610 620 630 640
QVMDAWLLDE HGKLKAEYAE PVAPLAAAVA KPEPTAAEPE APALEQ
Length:646
Mass (Da):72,213
Last modified:November 1, 1999 - v1
Checksum:i2F4CA6F5746CCCB1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF147448 Genomic DNA. Translation: AAD32230.1.
CP012901 Genomic DNA. Translation: ALV80466.1.
CP025055 Genomic DNA. Translation: AUA75512.1.
CVWF01000185 Genomic DNA. Translation: CRQ64476.1.
LLOD01000001 Genomic DNA. Translation: KSH18796.1.
NFFO01000063 Genomic DNA. Translation: OTH74495.1.
NFFZ01000017 Genomic DNA. Translation: OTI57375.1.
NMRQ01000014 Genomic DNA. Translation: OZO18476.1.
LT969520 Genomic DNA. Translation: SOV27592.1.
PIRiG83146.
RefSeqiNP_252692.1. NC_002516.2.
WP_003093191.1. NZ_PHST01000074.1.

Genome annotation databases

EnsemblBacteriaiCRO33333; CRO33333; PAERUG_P32_London_17_VIM_2_10_11_02137.
CRQ64476; CRQ64476; PAERUG_E15_London_28_01_14_04647.
KSH18796; KSH18796; AO964_03845.
GeneIDi878962.
KEGGipae:PA4003.
paeb:NCGM1900_0960.
PATRICifig|287.1477.peg.1343.

Similar proteinsi

Entry informationi

Entry nameiQ9X6V3_PSEAI
AccessioniPrimary (citable) accession number: Q9X6V3
Secondary accession number(s): Q7DC85
Entry historyiIntegrated into UniProtKB/TrEMBL: November 1, 1999
Last sequence update: November 1, 1999
Last modified: March 28, 2018
This is version 117 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported