Q9X6U2 (BDHA_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D-beta-hydroxybutyrate dehydrogenase
Short name=BDH
EC=1.1.1.30

Alternative name(s):
3-hydroxybutyrate dehydrogenase
Short name=3-HBDH

Gene names

Name:	hbdH1
Ordered Locus Names:	H16_A1334

Organism

Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]

Taxonomic identifier

381666 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	3-hydroxybutyrate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	D-beta-hydroxybutyrate dehydrogenase		PRO_0000054527
Regions
	Nucleotide binding	8 – 32	25	NAD By similarity
Sites
	Active site	153	1	Proton acceptor By similarity
	Binding site	140	1	Substrate By similarity
Experimental info
	Sequence conflict	236	1	L → Q in AAD33952. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9X6U2 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 0B9A06D6CD97EF35	FASTA	258	26,999
	10 20 30 40 50 60 MLNGKTALVT GSTSGIGLGI AKALAAQGAN IIVNGFGDAD AAKAEIAQAG QGIRVGYHGA 70 80 90 100 110 120 DMSKAAEIED MMRYAQSDFG GADILVNNAG IQHVAAIEDF PPERWDAIIA INLTSAFHTT 130 140 150 160 170 180 RLALPGMKQK DWGRIINVAS THGLVASAQK SAYVAAKHGI VGFTKVTALE TAQTGVTANA 190 200 210 220 230 240 ICPGWVLTPL VQKQVEARAQ KEGIPVEQAK RELVLEKQPS GQFVTPDELG ALAVFLSSEA 250 ARQVRGAIWN MDGGWVAQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the gene for beta-hydroxybutyrate dehydrogenase from Ralstonia eutropha." Kim J.W., Kang D.G., Rha E.G. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16." Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B. Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF145230 Genomic DNA. Translation: AAD33952.1. AM260479 Genomic DNA. Translation: CAJ92474.1.
RefSeq	YP_725842.1. NC_008313.1.
3D structure databases
ProteinModelPortal	Q9X6U2.
SMR	Q9X6U2. Positions 2-258.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9X6U2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4249460.
GenomeReviews	Gene locus H16_A1334 in contig AM260479_GR.
KEGG	reh:H16_A1334.
PATRIC	35232127. VBIRalEut6770_1725.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1028.
HOGENOM	HBG750976.
OMA	IEDMMRY.
PhylomeDB	Q9X6U2.
ProtClustDB	PRK12429.
Enzyme and pathway databases
BioCyc	REUT381666:H16_A1334-MONOMER.
Family and domain databases
InterPro	IPR011294. 3-OHbutyrate_DH. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00019.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01963. PHB_DH. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BDHA_CUPNH

Accession

Primary (citable) accession number: Q9X6U2
Secondary accession number(s): Q0KBZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program