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Q9X6U2 (BDHA_CUPNH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 70. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-beta-hydroxybutyrate dehydrogenase

Short name=BDH
EC=1.1.1.30
Alternative name(s):
3-hydroxybutyrate dehydrogenase
Short name=3-HBDH
Gene names
Name:hbdH1
Ordered Locus Names:H16_A1334
OrganismCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha) [Complete proteome] [HAMAP]
Taxonomic identifier381666 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus

Protein attributes

Sequence length258 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

(R)-3-hydroxybutanoate + NAD+ = acetoacetate + NADH.

Sequence similarities

Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular function3-hydroxybutyrate dehydrogenase activity

Inferred from electronic annotation. Source: EC

nucleotide binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 258258D-beta-hydroxybutyrate dehydrogenase
PRO_0000054527

Regions

Nucleotide binding8 – 3225NAD By similarity

Sites

Active site1531Proton acceptor By similarity
Binding site1401Substrate By similarity

Experimental info

Sequence conflict2361L → Q in AAD33952. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9X6U2 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 0B9A06D6CD97EF35

FASTA25826,999
        10         20         30         40         50         60 
MLNGKTALVT GSTSGIGLGI AKALAAQGAN IIVNGFGDAD AAKAEIAQAG QGIRVGYHGA 

        70         80         90        100        110        120 
DMSKAAEIED MMRYAQSDFG GADILVNNAG IQHVAAIEDF PPERWDAIIA INLTSAFHTT 

       130        140        150        160        170        180 
RLALPGMKQK DWGRIINVAS THGLVASAQK SAYVAAKHGI VGFTKVTALE TAQTGVTANA 

       190        200        210        220        230        240 
ICPGWVLTPL VQKQVEARAQ KEGIPVEQAK RELVLEKQPS GQFVTPDELG ALAVFLSSEA 

       250 
ARQVRGAIWN MDGGWVAQ 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of the gene for beta-hydroxybutyrate dehydrogenase from Ralstonia eutropha."
Kim J.W., Kang D.G., Rha E.G.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia eutropha H16."
Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R., Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I., Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.
Nat. Biotechnol. 24:1257-1262(2006) [PubMed: 16964242] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF145230 Genomic DNA. Translation: AAD33952.1.
AM260479 Genomic DNA. Translation: CAJ92474.1.
RefSeqYP_725842.1. NC_008313.1.

3D structure databases

ProteinModelPortalQ9X6U2.
SMRQ9X6U2. Positions 2-258.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ9X6U2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4249460.
GenomeReviewsGene locus H16_A1334 in contig AM260479_GR.
KEGGreh:H16_A1334.
PATRIC35232127. VBIRalEut6770_1725.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1028.
HOGENOMHBG750976.
OMAIEDMMRY.
PhylomeDBQ9X6U2.
ProtClustDBPRK12429.

Enzyme and pathway databases

BioCycREUT381666:H16_A1334-MONOMER.

Family and domain databases

InterProIPR011294. 3-OHbutyrate_DH.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK00019.
PfamPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsTIGR01963. PHB_DH. 1 hit.
PROSITEPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBDHA_CUPNH
AccessionPrimary (citable) accession number: Q9X6U2
Secondary accession number(s): Q0KBZ7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families