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Protein

Ribosomal RNA small subunit methyltransferase J

Gene

rsmJ

Organism
Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the guanosine in position 1516 of 16S rRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(1516) in 16S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(1516) in 16S rRNA.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei171 – 1711S-adenosyl-L-methionineUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3616-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase JUniRule annotation (EC:2.1.1.242UniRule annotation)
Alternative name(s):
16S rRNA m2G1516 methyltransferaseUniRule annotation
rRNA (guanine-N(2)-)-methyltransferaseUniRule annotation
Gene namesi
Name:rsmJUniRule annotation
Synonyms:yhiQ
Ordered Locus Names:STM3593
OrganismiSalmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
Taxonomic identifieri99287 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella
Proteomesi
  • UP000001014 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 252252Ribosomal RNA small subunit methyltransferase JPRO_0000212091Add
BLAST

Proteomic databases

PaxDbiQ9X6G2.
PRIDEiQ9X6G2.

Interactioni

Protein-protein interaction databases

STRINGi99287.STM3593.

Structurei

Secondary structure

1
252
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi15 – 239Combined sources
Beta strandi32 – 376Combined sources
Beta strandi42 – 465Combined sources
Helixi50 – 523Combined sources
Beta strandi59 – 613Combined sources
Helixi62 – 709Combined sources
Helixi73 – 753Combined sources
Helixi77 – 815Combined sources
Beta strandi92 – 943Combined sources
Helixi101 – 1099Combined sources
Beta strandi113 – 1175Combined sources
Helixi120 – 13516Combined sources
Beta strandi137 – 1393Combined sources
Helixi140 – 1467Combined sources
Beta strandi147 – 1526Combined sources
Turni154 – 1563Combined sources
Beta strandi166 – 1705Combined sources
Turni179 – 1824Combined sources
Helixi185 – 19410Combined sources
Helixi200 – 2034Combined sources
Helixi204 – 2107Combined sources
Beta strandi212 – 2209Combined sources
Helixi226 – 2283Combined sources
Beta strandi234 – 2363Combined sources
Beta strandi238 – 2469Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PKWX-ray2.10A1-252[»]
ProteinModelPortaliQ9X6G2.
SMRiQ9X6G2. Positions 1-252.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X6G2.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni101 – 1022S-adenosyl-L-methionine bindingUniRule annotation
Regioni117 – 1182S-adenosyl-L-methionine bindingUniRule annotation
Regioni153 – 1542S-adenosyl-L-methionine bindingUniRule annotation

Sequence similaritiesi

Belongs to the methyltransferase superfamily. RsmJ family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.
PhylomeDBiQ9X6G2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X6G2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQICLMDETG ATDGALSVLA ARWGLEHDED NPMALVMTPQ HLELRKRDEP
60 70 80 90 100
KLGGIFVDFV GGAMAHRRKF GGGRGEAVAK AVGIKGDYLP DVVDATAGLG
110 120 130 140 150
RDAFVLASVG CRVRMLERNP VVAALLDDGL TRGYADADIG GWLQERLQLI
160 170 180 190 200
HASSLTALTD ITPRPQVVYL DPMFPHRQKS ALVKKEMRVF QSLVGPDLDA
210 220 230 240 250
DGLLEPARQL ATKRVVVKRP DYAPPLADVA TPNAIVTKGH RFDIYAGTPL

TE
Length:252
Mass (Da):27,300
Last modified:January 23, 2002 - v2
Checksum:i3037E3B43E070046
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti225 – 2251P → PP in AAD31478 (PubMed:10629202).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF137028 Genomic DNA. Translation: AAD31478.1.
AE006468 Genomic DNA. Translation: AAL22453.1.
RefSeqiNP_462494.1. NC_003197.1.
WP_001165134.1. NC_003197.1.

Genome annotation databases

EnsemblBacteriaiAAL22453; AAL22453; STM3593.
GeneIDi1255116.
KEGGistm:STM3593.
PATRICi32385999. VBISalEnt20916_3797.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF137028 Genomic DNA. Translation: AAD31478.1.
AE006468 Genomic DNA. Translation: AAL22453.1.
RefSeqiNP_462494.1. NC_003197.1.
WP_001165134.1. NC_003197.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2PKWX-ray2.10A1-252[»]
ProteinModelPortaliQ9X6G2.
SMRiQ9X6G2. Positions 1-252.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi99287.STM3593.

Proteomic databases

PaxDbiQ9X6G2.
PRIDEiQ9X6G2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL22453; AAL22453; STM3593.
GeneIDi1255116.
KEGGistm:STM3593.
PATRICi32385999. VBISalEnt20916_3797.

Phylogenomic databases

eggNOGiENOG4105FFN. Bacteria.
ENOG410XPRJ. LUCA.
HOGENOMiHOG000218236.
KOiK15984.
OMAiSSRYDIY.
PhylomeDBiQ9X6G2.

Enzyme and pathway databases

BioCyciSENT99287:GCTI-3616-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9X6G2.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01523. 16SrRNA_methyltr_J. 1 hit.
InterProiIPR007536. 16SrRNA_methylTrfase_J.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF04445. SAM_MT. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRSMJ_SALTY
AccessioniPrimary (citable) accession number: Q9X6G2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2002
Last modified: September 7, 2016
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.