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Q9X6C6 (BGAL_THEBO) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase BgaT

Short name=Beta-gal
EC=3.2.1.23
Gene names
Name:bgaT
OrganismThermus brockianus
Taxonomic identifier56956 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Protein attributes

Sequence length645 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes pNP-beta-galactoside. Also possesses activity against galactose, and to a lesser extent towards melibiose and lactose.

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. Ref.2

Sequence similarities

Belongs to the glycosyl hydrolase 42 family.

Ontologies

Keywords
   LigandMetal-binding
Zinc
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processgalactose metabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentbeta-galactosidase complex

Inferred from electronic annotation. Source: InterPro

   Molecular_functionbeta-galactosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 645645Beta-galactosidase BgaT
PRO_0000407694

Regions

Region360 – 3634Substrate binding

Sites

Active site1411Proton donor By similarity
Active site3121Nucleophile By similarity
Metal binding1061Zinc By similarity
Metal binding1501Zinc By similarity
Metal binding1521Zinc By similarity
Metal binding1551Zinc By similarity
Binding site1021Substrate By similarity
Binding site1401Substrate By similarity
Binding site3201Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X6C6 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: C79A9E1C0020EC40

FASTA64573,421
        10         20         30         40         50         60 
MLGVCYYPEH WPRERWSEDA RRMRELGLAY VRVGEFAWAL LEPEPGRLDW AWLDEAVAVL 

        70         80         90        100        110        120 
AQAGLKVVLG TPTATPPKWL VDRYPEILPV DREGRRRRFG GRRHYCFSSP VYHEETRRIV 

       130        140        150        160        170        180 
TLLGERYGKH PAVAGFQTDN EYGCHGTVRC YCERCQDAFR KWLEERYGSI DVLNEAWGTV 

       190        200        210        220        230        240 
FWSQRYRTFQ EVELPNLTVA EANPSHLLDY YRFASEQVRR YNRLQVEILR AHAPGKFVTH 

       250        260        270        280        290        300 
NFMGFFTDLN PFPLGEDLDF ASWDSYPLGF TDLMPLPEEE KLRYARTGHP DVAAFHHDLY 

       310        320        330        340        350        360 
RAVGRGRFWV MEQQPGPVNW APHNPNPAPG MVRLWTWEAL ANGAEVVSYF RWRQVPFAQE 

       370        380        390        400        410        420 
QMHAGLHRPD YAPDAAFFEV QRVVEELGAL SLPPPGQAPV ALVYDPEAPW VYEVQPHGAE 

       430        440        450        460        470        480 
WNYLALVFLF YSVARRLGLD VDIVPPGAAL QGYRLVLVPS LPIVREKALN AFREADGIVL 

       490        500        510        520        530        540 
FGPRSGSKNE NFHIPQGLPP GPLQALLPLK VVRVESLPPG LREEVEGPWG RFSSGLWREW 

       550        560        570        580        590        600 
VETDLSPLLR FADGLGALFR AGRYLYLAAW PSPELLGALL VGLAQEGGLS PKPLPSGLRL 

       610        620        630        640 
RWRGHLVFAF NYGPEEVVLP VPSGVRFRLG GPRLSPYEVA VWEEG 

« Hide

References

[1]"Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360."
Fridjonsson O., Watzlawick H., Gehweiler A., Rohrhirsch T., Mattes R.
Appl. Environ. Microbiol. 65:3955-3963(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ITI360.
[2]"The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125."
Fridjonsson O., Watzlawick H., Mattes R.
Extremophiles 4:23-33(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY.
Strain: ITI360.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF135398 Genomic DNA. Translation: AAD33667.1.

3D structure databases

ProteinModelPortalQ9X6C6.
SMRQ9X6C6. Positions 1-644.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFPIRSF001084. B-galactosidase. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBGAL_THEBO
AccessionPrimary (citable) accession number: Q9X6C6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 1, 1999
Last modified: October 16, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries