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Protein

Beta-galactosidase BgaT

Gene

bgaT

Organism
Thermus brockianus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes pNP-beta-galactoside. Also possesses activity against galactose, and to a lesser extent towards melibiose and lactose.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei102SubstrateBy similarity1
Metal bindingi106ZincBy similarity1
Binding sitei140SubstrateBy similarity1
Active sitei141Proton donorBy similarity1
Metal bindingi150ZincBy similarity1
Metal bindingi152ZincBy similarity1
Metal bindingi155ZincBy similarity1
Active sitei312NucleophileBy similarity1
Binding sitei320SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaT (EC:3.2.1.23)
Short name:
Beta-galBy similarity
Gene namesi
Name:bgaTImported
OrganismiThermus brockianus
Taxonomic identifieri56956 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004076941 – 645Beta-galactosidase BgaTAdd BLAST645

Structurei

3D structure databases

ProteinModelPortaliQ9X6C6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni360 – 363Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the glycosyl hydrolase 42 family.Sequence analysis

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X6C6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLGVCYYPEH WPRERWSEDA RRMRELGLAY VRVGEFAWAL LEPEPGRLDW
60 70 80 90 100
AWLDEAVAVL AQAGLKVVLG TPTATPPKWL VDRYPEILPV DREGRRRRFG
110 120 130 140 150
GRRHYCFSSP VYHEETRRIV TLLGERYGKH PAVAGFQTDN EYGCHGTVRC
160 170 180 190 200
YCERCQDAFR KWLEERYGSI DVLNEAWGTV FWSQRYRTFQ EVELPNLTVA
210 220 230 240 250
EANPSHLLDY YRFASEQVRR YNRLQVEILR AHAPGKFVTH NFMGFFTDLN
260 270 280 290 300
PFPLGEDLDF ASWDSYPLGF TDLMPLPEEE KLRYARTGHP DVAAFHHDLY
310 320 330 340 350
RAVGRGRFWV MEQQPGPVNW APHNPNPAPG MVRLWTWEAL ANGAEVVSYF
360 370 380 390 400
RWRQVPFAQE QMHAGLHRPD YAPDAAFFEV QRVVEELGAL SLPPPGQAPV
410 420 430 440 450
ALVYDPEAPW VYEVQPHGAE WNYLALVFLF YSVARRLGLD VDIVPPGAAL
460 470 480 490 500
QGYRLVLVPS LPIVREKALN AFREADGIVL FGPRSGSKNE NFHIPQGLPP
510 520 530 540 550
GPLQALLPLK VVRVESLPPG LREEVEGPWG RFSSGLWREW VETDLSPLLR
560 570 580 590 600
FADGLGALFR AGRYLYLAAW PSPELLGALL VGLAQEGGLS PKPLPSGLRL
610 620 630 640
RWRGHLVFAF NYGPEEVVLP VPSGVRFRLG GPRLSPYEVA VWEEG
Length:645
Mass (Da):73,421
Last modified:November 1, 1999 - v1
Checksum:iC79A9E1C0020EC40
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135398 Genomic DNA. Translation: AAD33667.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF135398 Genomic DNA. Translation: AAD33667.1.

3D structure databases

ProteinModelPortaliQ9X6C6.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBGAL_THEBO
AccessioniPrimary (citable) accession number: Q9X6C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 1, 1999
Last modified: October 5, 2016
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.