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Q9X6C6

- BGAL_THEBO

UniProt

Q9X6C6 - BGAL_THEBO

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Protein

Beta-galactosidase BgaT

Gene
bgaT
Organism
Thermus brockianus
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes pNP-beta-galactoside. Also possesses activity against galactose, and to a lesser extent towards melibiose and lactose.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei102 – 1021Substrate By similarity
Metal bindingi106 – 1061Zinc By similarity
Binding sitei140 – 1401Substrate By similarity
Active sitei141 – 1411Proton donor By similarity
Metal bindingi150 – 1501Zinc By similarity
Metal bindingi152 – 1521Zinc By similarity
Metal bindingi155 – 1551Zinc By similarity
Active sitei312 – 3121Nucleophile By similarity
Binding sitei320 – 3201Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. galactose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

CAZyiGH42. Glycoside Hydrolase Family 42.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase BgaT (EC:3.2.1.23)
Short name:
Beta-gal
Gene namesi
Name:bgaT
OrganismiThermus brockianus
Taxonomic identifieri56956 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 645645Beta-galactosidase BgaTPRO_0000407694Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9X6C6.
SMRiQ9X6C6. Positions 1-644.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni360 – 3634Substrate binding

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProiIPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view]
PIRSFiPIRSF001084. B-galactosidase. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9X6C6-1 [UniParc]FASTAAdd to Basket

« Hide

MLGVCYYPEH WPRERWSEDA RRMRELGLAY VRVGEFAWAL LEPEPGRLDW    50
AWLDEAVAVL AQAGLKVVLG TPTATPPKWL VDRYPEILPV DREGRRRRFG 100
GRRHYCFSSP VYHEETRRIV TLLGERYGKH PAVAGFQTDN EYGCHGTVRC 150
YCERCQDAFR KWLEERYGSI DVLNEAWGTV FWSQRYRTFQ EVELPNLTVA 200
EANPSHLLDY YRFASEQVRR YNRLQVEILR AHAPGKFVTH NFMGFFTDLN 250
PFPLGEDLDF ASWDSYPLGF TDLMPLPEEE KLRYARTGHP DVAAFHHDLY 300
RAVGRGRFWV MEQQPGPVNW APHNPNPAPG MVRLWTWEAL ANGAEVVSYF 350
RWRQVPFAQE QMHAGLHRPD YAPDAAFFEV QRVVEELGAL SLPPPGQAPV 400
ALVYDPEAPW VYEVQPHGAE WNYLALVFLF YSVARRLGLD VDIVPPGAAL 450
QGYRLVLVPS LPIVREKALN AFREADGIVL FGPRSGSKNE NFHIPQGLPP 500
GPLQALLPLK VVRVESLPPG LREEVEGPWG RFSSGLWREW VETDLSPLLR 550
FADGLGALFR AGRYLYLAAW PSPELLGALL VGLAQEGGLS PKPLPSGLRL 600
RWRGHLVFAF NYGPEEVVLP VPSGVRFRLG GPRLSPYEVA VWEEG 645
Length:645
Mass (Da):73,421
Last modified:November 1, 1999 - v1
Checksum:iC79A9E1C0020EC40
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF135398 Genomic DNA. Translation: AAD33667.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF135398 Genomic DNA. Translation: AAD33667.1 .

3D structure databases

ProteinModelPortali Q9X6C6.
SMRi Q9X6C6. Positions 1-644.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH42. Glycoside Hydrolase Family 42.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
3.40.50.880. 1 hit.
InterProi IPR013739. Beta_galactosidase_C.
IPR013738. Beta_galactosidase_Trimer.
IPR029062. Class_I_gatase-like.
IPR013780. Glyco_hydro_13_b.
IPR003476. Glyco_hydro_42.
IPR013529. Glyco_hydro_42_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02449. Glyco_hydro_42. 1 hit.
PF08533. Glyco_hydro_42C. 1 hit.
PF08532. Glyco_hydro_42M. 1 hit.
[Graphical view ]
PIRSFi PIRSF001084. B-galactosidase. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF52317. SSF52317. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360."
    Fridjonsson O., Watzlawick H., Gehweiler A., Rohrhirsch T., Mattes R.
    Appl. Environ. Microbiol. 65:3955-3963(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ITI360.
  2. "The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125."
    Fridjonsson O., Watzlawick H., Mattes R.
    Extremophiles 4:23-33(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY.
    Strain: ITI360.

Entry informationi

Entry nameiBGAL_THEBO
AccessioniPrimary (citable) accession number: Q9X6C6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 3, 2011
Last sequence update: November 1, 1999
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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