Reviewed, UniProtKB/Swiss-Prot Q9X6B0 (KATG_YERPE)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase
    Antigen 5

Gene names

Name:	katG
Synonyms:	katY
Ordered Locus Names:	YPO3319, y0870, YP_0367

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	737 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homotetramer. Ref.1
Subcellular location	Periplasm. Ref.1
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Cellular component	Periplasm
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Ref.1
	Chain	24 – 737	714	Catalase-peroxidase HAMAP MF_01961		PRO_0000055578
Sites
	Active site	103	1	Proton acceptor By similarity
	Metal binding	264	1	Iron (heme axial ligand) By similarity
	Site	99	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	102 ↔ 223		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-249) By similarity
	Cross-link	223 ↔ 249		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-102) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X6B0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: DB870BC41ABD469A	FASTA	737	81,365
	10 20 30 40 50 60 MLKKILPVLI TLAIVHNTPT AWAAEAPKTD SFYLPKSLDL SPLRLHNIES NPYGKDFNYA 70 80 90 100 110 120 QQFKTLDLEA VKKDIKTVLT TSQDWWPADY GNYGPFFIRM AWHGAGTYRI YDGRGGADGG 130 140 150 160 170 180 QQRFEPLNSW PDNANLDKAR RLLWPIKKKY GAKISWGDLM VLTGNVALES MGFKTLGFAG 190 200 210 220 230 240 GREDDWQSDL VYWGAGNKML SDNRDKNGKL PKPLAATQMG LIYVNPEGPN GKPDPVAAAK 250 260 270 280 290 300 DIREAFARMA MNDEETVALI AGGHTFGKAH GAASPEKCLG AAPGEAGLEQ QGLGWANKCG 310 320 330 340 350 360 SGNGKDTITS GLEGAWTTDP THFTMQYLSN LYKHEWVLTK SPAGAWQWKP KNAANVVPDA 370 380 390 400 410 420 TDPTKFHPLM MFTTDIALKV DPEYKKITTR FLENPEEFKM AFARAWFKLT HRDMGPAARY 430 440 450 460 470 480 LGDEVPKETF IWQDPLPAAN YKMIDSADIS ELKDKILKTG LSDTKLIKTA WASASTFRGT 490 500 510 520 530 540 DFRGGDNGAR IRLAPQKDWP VNDPAELHSV LAALMEVQNN FNKDRSDGKK VSLSDLIVLG 550 560 570 580 590 600 GNAAIEDAAK KAGYSISIPF TPGRTDASQE ETDVSSFAVL EPTADGFRNY YDAKRNTLSP 610 620 630 640 650 660 IASLIDRANK LELTVPEMTV LIGGLRVLDV NSGGSKAGVL TNTPGQLNNN FFVNLLDMST 670 680 690 700 710 720 KWTKSPKAEG YFDGYDRKTG KLKWTASSVD LVFGSNPELR AVAEVYASDD AKEKFVHDFT 730 KVWEKVMNLD RFDIKNN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of KatY (antigen 5), a thermoregulated chromosomally encoded catalase-peroxidase of Yersinia pestis." Garcia E., Nedialkov Y.A., Elliott J., Motin V.L., Brubaker R.R. J. Bacteriol. 181:3114-3122(1999) [PubMed: 10322012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 24-31 AND 250-254, SUBCELLULAR LOCATION, SUBUNIT. Strain: KIM.
[2]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[3]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[4]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF135170 Genomic DNA. Translation: AAD37313.1. AL590842 Genomic DNA. Translation: CAL21910.1. AE009952 Genomic DNA. Translation: AAM84455.1. Different initiation. AE017042 Genomic DNA. Translation: AAS60640.1. Different initiation.
PIR	AC0403.
RefSeq	NP_668204.1. NP_991763.1. YP_002348215.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2640. YpCP01.
Genome annotation databases
GeneID	1145817. 1176138. 2764071.
GenomeReviews	Gene locus y0870 in contig AE009952_GR. Gene locus YP_0367 in contig AE017042_GR. Gene locus YPO3319 in contig AL590842_GR.
KEGG	ype:YPO3319. ypk:y0870. ypm:YP_0367.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9X6B0.
OMA	Q9X6B0. GTDMRGG.
Enzyme and pathway databases
BioCyc	YPES187410:Y0870-MON. YPES214092:YPO3319-MON. YPES229193:YP0367-MON.
BRENDA	1.11.1.6. 142588.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_YERPE

Accession

Primary (citable) accession number: Q9X6B0
Secondary accession number(s): Q0WBX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1999
Last modified:	June 16, 2009
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)