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Protein

Copper-transporting P-type ATPase

Gene

actP

Organism
Sinorhizobium medicae (strain WSM419) (Ensifer medicae)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in copper efflux.

Catalytic activityi

ATP + H2O + Cu2+(Side 1) = ADP + phosphate + Cu2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261CopperPROSITE-ProRule annotation
Metal bindingi29 – 291CopperPROSITE-ProRule annotation
Metal bindingi93 – 931CopperPROSITE-ProRule annotation
Metal bindingi96 – 961CopperPROSITE-ProRule annotation
Active sitei515 – 51514-aspartylphosphate intermediateBy similarity
Metal bindingi714 – 7141MagnesiumPROSITE-ProRule annotation
Metal bindingi718 – 7181MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Copper transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Copper, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSMED366394:GJAL-4959-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Copper-transporting P-type ATPase (EC:3.6.3.4)
Gene namesi
Name:actP
Ordered Locus Names:Smed_4897
Encoded oniPlasmid pSMED010 Publication
OrganismiSinorhizobium medicae (strain WSM419) (Ensifer medicae)
Taxonomic identifieri366394 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001108 Componenti: Plasmid pSMED01

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Transmembranei210 – 23021HelicalSequence analysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence analysisAdd
BLAST
Transmembranei271 – 29121HelicalSequence analysisAdd
BLAST
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Transmembranei458 – 47821HelicalSequence analysisAdd
BLAST
Transmembranei541 – 56121HelicalSequence analysisAdd
BLAST
Transmembranei569 – 58921HelicalSequence analysisAdd
BLAST
Transmembranei727 – 74721HelicalSequence analysisAdd
BLAST
Transmembranei773 – 79321HelicalSequence analysisAdd
BLAST
Transmembranei795 – 81521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 827827Copper-transporting P-type ATPasePRO_0000046329Add
BLAST

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Transcriptionally regulated by HmrR in response to Cu+ ions.

Structurei

3D structure databases

ProteinModelPortaliQ9X5X3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 8166HMA 1PROSITE-ProRule annotationAdd
BLAST
Domaini83 – 14967HMA 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 HMA domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000250397.
KOiK17686.
OMAiKTGYEAR.
OrthoDBiEOG6742RM.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 2 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
TIGR00003. TIGR00003. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X5X3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALKQTEKS TSLPMSFDFD IEGMTCASCV RRVEKAIAAV PGVASANVNL
60 70 80 90 100
ATERATVQFN GVPETTSVLR AVEKAGYAPR IVTEEIQIEG MTCASCVSRV
110 120 130 140 150
EKALKAVPGV ADASVNLATE KATVRLVSGS AEISALAAAV KGAGYGIRKA
160 170 180 190 200
TPAEAMKEDV DHRTAELRSL KSAVTISSLM TLPLFLLEMG SHFIPGVHDF
210 220 230 240 250
IMGTIGMRNN LYLQFALATL VLFGPGLRFF RKGVPNLLRW TPDMNSLVVL
260 270 280 290 300
GTTAAWGYSV VTTFVPAILP SGTANVYYEA AAVIVTLILV GRYLESRAKG
310 320 330 340 350
RTSQAIKRLV GLQPKTAFVL HSGEFVETEI TEVVTGDVIR IRPGEKIPVD
360 370 380 390 400
GTVTDGSSYV DESMITGEPV PVQKATDSAV IGGTINKTGS ITFKATKVGS
410 420 430 440 450
DTLLAQIIRL VEAAQGSKLP IQALVDRVTA WFVPVVILAA LLTFAAWYVL
460 470 480 490 500
GPSPALSFAL VNAVAVLIIA CPCAMGLATP TSIMVGTGRA AELGILFRKG
510 520 530 540 550
EALQSLRDAD VVAVDKTGTL TKGRPELTDL VAAEGFEPDE VLCLVASLET
560 570 580 590 600
LSEHPIAEAI VSAAKSRGIA TVAVSAFEAT PGFGVSGTVS GRRVLVGADR
610 620 630 640 650
ALVKNGIDIT GFADEAERLG SGGKSPLYAA IDGRLAAIVA VSDPVKESTP
660 670 680 690 700
QAIKSLHALG LKVAMVTGDN RRTAEAIAKK LGIDEVVAEV LPEGKVDAVR
710 720 730 740 750
KLRQGGRSVA FIGDGINDAP ALAEADVGIA VGTGTDIAIE SADVVLMSGD
760 770 780 790 800
LNGVAKALAL SKATIRNIKQ NLFWAFVYNI SLVPVAAGVL YPVNGTLLSP
810 820
IFAAAAMAMS SVFVLGNALR LKSFDPA
Length:827
Mass (Da):86,239
Last modified:November 1, 1999 - v1
Checksum:i707E2148DDDA5004
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129004 Genomic DNA. Translation: AAD27639.1.
CP000739 Genomic DNA. Translation: ABR63681.1.
RefSeqiWP_012061811.1. NC_009620.1.
YP_001313614.1. NC_009620.1.

Genome annotation databases

EnsemblBacteriaiABR63681; ABR63681; Smed_4897.
GeneIDi5318235.
KEGGismd:Smed_4897.
PATRICi23615407. VBISinMed134228_1375.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF129004 Genomic DNA. Translation: AAD27639.1.
CP000739 Genomic DNA. Translation: ABR63681.1.
RefSeqiWP_012061811.1. NC_009620.1.
YP_001313614.1. NC_009620.1.

3D structure databases

ProteinModelPortaliQ9X5X3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABR63681; ABR63681; Smed_4897.
GeneIDi5318235.
KEGGismd:Smed_4897.
PATRICi23615407. VBISinMed134228_1375.

Phylogenomic databases

HOGENOMiHOG000250397.
KOiK17686.
OMAiKTGYEAR.
OrthoDBiEOG6742RM.

Enzyme and pathway databases

BioCyciSMED366394:GJAL-4959-MONOMER.

Family and domain databases

Gene3Di2.70.150.10. 1 hit.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR017969. Heavy-metal-associated_CS.
IPR006122. HMA_Cu_ion-bd.
IPR006121. HMA_dom.
IPR027256. P-typ_ATPase_IB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF00403. HMA. 2 hits.
[Graphical view]
PRINTSiPR00941. CDATPASE.
SUPFAMiSSF55008. SSF55008. 2 hits.
SSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01525. ATPase-IB_hvy. 1 hit.
TIGR01494. ATPase_P-type. 1 hit.
TIGR00003. TIGR00003. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
PS01047. HMA_1. 2 hits.
PS50846. HMA_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "ActP controls copper homeostasis in Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti preventing low pH-induced copper toxicity."
    Reeve W.G., Tiwari R.P., Kale N.B., Dilworth M.J., Glenn A.R.
    Mol. Microbiol. 43:981-991(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "Complete sequence of Sinorhizobium medicae WSM419 plasmid pSMED01."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Reeve W.G., Richardson P.
    Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: WSM419.

Entry informationi

Entry nameiATCU_SINMW
AccessioniPrimary (citable) accession number: Q9X5X3
Secondary accession number(s): A6UJ51
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: April 13, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.