Reviewed, UniProtKB/Swiss-Prot Q9X5V3 (ATCU_RHILV)

Names and origin

Protein names

Recommended name:
    Copper-transporting P-type ATPase
    EC=3.6.3.4

Gene names

Name:

actP

Organism

Rhizobium leguminosarum bv. viciae

Taxonomic identifier

387 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Rhizobium

Protein attributes

Sequence length	841 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in copper efflux.
Catalytic activity	ATP + H(2)O + Cu(2+)(In) = ADP + phosphate + Cu(2+)(Out).
Subcellular location	Cell membrane; Multi-pass membrane proteinProbable.
Induction	Transcriptionally regulated by hmrR in response to Cu(+) ions.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IB subfamily.

Ontologies

Keywords
Biological process	Copper transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	ATP-binding Copper Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	copper ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 841	841	Copper-transporting P-type ATPase		PRO_0000046326
Regions
	Transmembrane	186 – 206	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	256 – 276	21	Potential
	Transmembrane	285 – 305	21	Potential
	Transmembrane	445 – 465	21	Potential
	Transmembrane	474 – 494	21	Potential
	Transmembrane	602 – 622	21	Potential
	Transmembrane	638 – 658	21	Potential
	Transmembrane	742 – 762	21	Potential
	Transmembrane	800 – 820	21	Potential
	Compositional bias	9 – 24	16	His-rich
Sites
	Active site	530	1	4-aspartylphosphate intermediate By similarity
	Metal binding	729	1	Magnesium By similarity
	Metal binding	733	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X5V3-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: F6BE9E88F4C7BD7D	FASTA	841	88,059
	10 20 30 40 50 60 MNIKQEDDHH HSHAHGDNHC HCGHDQEKAA DAIVRDPICG MTVDPQAGKP SLGHGGRIYH 70 80 90 100 110 120 FCSEHCRTKF AAAPEDYLTA KDPVCGMSVD RSTARYFLKA EGEKFYFCSA ACQAKFEADP 130 140 150 160 170 180 AAYRDGQRPT AKPAPKGTLY TCPMHPEVVS DRPGDCPKCG MALEPMGIPP TDEGPNPELV 190 200 210 220 230 240 DFVRRLWVSA ILALPLLALG MGPMLGLPLR EAIGEPQATF IELLLATPVV LWAALPFFRR 250 260 270 280 290 300 AWASVVNRSP NMWTLIGLGV GTAYLYSVVA TLAPGIFPMS FRGHGAAVPV YFEAAAVIVA 310 320 330 340 350 360 LVFVGQVLEL KARERTGSAI RALLDLAPKT ARRIDAEGNE SDVPVDDINV ADRLRVRPGE 370 380 390 400 410 420 RVPVDGSVLE GQSTVDESMI SGEPLPVEKS KGDPLTGGTI NKNGTFVMSA EKVGADTVLS 430 440 450 460 470 480 RIVDMVAKAQ RSRAPIQGAV DRVSAVFVPA VVAVALLAFL AWAAIGPEPR MANGLLAAVA 490 500 510 520 530 540 VLIIACPCAL GLATPMSIMI ATGRGAGEGV LIKDAEALER FSKGDTLIVD KTGTLTEGKP 550 560 570 580 590 600 KLTDIAAFGR VGEDRLLSLA ASLERGSEHP LAEAIVSGAE ERGVPFVEVT GFEAKTGKGV 610 620 630 640 650 660 QGIADGTMVA LGNSAMLADL GIDPAALSEK TEALRGDGKT VMFVVFDGAL AGLVAVADRI 670 680 690 700 710 720 KPTTAAAIQA LHDSGLKIIM ATGDNERTAR AVAKSLGIDE VRADVLPEGK KALIDELRSK 730 740 750 760 770 780 GAIIAMAGDG VNDAPALAAA DVGIAMGTGA DVAMESAGIT LVKGDLTGIV RARRLAEATM 790 800 810 820 830 840 RNIRQNLGFA FGYNALGVPV AAGVLYPILG LLLSPMIAAA AMSLSSVSVI SNALRLRFAK L

References

[1]

"ActP controls copper homeostasis in Rhizobium leguminosarum bv. viciae and Sinorhizobium meliloti preventing low pH-induced copper toxicity." Reeve W.G., Tiwari R.P., Kale N.B., Dilworth M.J., Glenn A.R. Mol. Microbiol. 43:981-991(2002) [PubMed: 11936079] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: WSM710.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF127795 Genomic DNA. Translation: AAD26860.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006416. ATPase-IB_hvy. IPR001757. ATPase_P. IPR006403. ATPase_P_cat/Cu. IPR001756. ATPase_P_Cu. IPR005834. Dehalogen-like_hydro. IPR008250. E1-E2_ATPase_reg. IPR011017. TRASH. IPR007029. YHS. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. PF04945. YHS. 2 hits. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00943. CUATPASE.
SMART	SM00746. TRASH. 2 hits. [Graphical view]
TIGRFAMs	TIGR01511. ATPase-IB1_Cu. 1 hit. TIGR01525. ATPase-IB_hvy. 1 hit. TIGR01494. ATPase_P-type. 2 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

ATCU_RHILV

Accession

Primary (citable) accession number: Q9X5V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	November 4, 2008
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)