Q9X5M0 (SYR_MYCS2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arginine--tRNA ligase
EC=6.1.1.19

Alternative name(s):
Arginyl-tRNA synthetase
Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	MSMEG_4959

Organism

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

Taxonomic identifier

246196 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW arginine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Arginine--tRNA ligase HAMAP MF_00123		PRO_0000151579
Regions
	Motif	130 – 140	11	"HIGH" region HAMAP MF_00123
Experimental info
	Sequence conflict	149	1	A → T in AAD32590. Ref.1
	Sequence conflict	232	1	D → N in AAD32590. Ref.1
	Sequence conflict	255 – 257	3	EDS → GDW in AAD32590. Ref.1
	Sequence conflict	331	1	L → V in AAD32590. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9X5M0 [UniParc]. Last modified July 10, 2007. Version 2. Checksum: 4DAB7844FFFCFCEC	FASTA	550	59,429
	10 20 30 40 50 60 MTPADLAELL KATAAAVLTE HDLDVAALPA TVTVERPRNP EHGDYATNLA LQLGKKVGVN 70 80 90 100 110 120 PRELAGWLAT ALTAADGIAV AEVAGPGFVN LRIEASAQGV IITNVLAAEG SYGSSDQYAG 130 140 150 160 170 180 RNVNLEFVSA NPTGPIHIGG TRWAAVGDAL GRLLATQGAA VTREYYFNDH GAQIDRFVNS 190 200 210 220 230 240 LIASAKGEPT PEDGYAGDYI VDIAQQVIAK APDVLGLPED QQRETFRAIG VDLMFTHIKQ 250 260 270 280 290 300 SLHDFGTDFD VYTHEDSMHT SGRVDQAITQ LREAGSIYEK DGAVWLRTTD FGDDKDRVVI 310 320 330 340 350 360 KSDGNAAYIA GDLAYYLDKR KRGFDLCIYM LGADHHGYIA RLKAAAAALG DDPDTVEVLI 370 380 390 400 410 420 GQMVNLVRDG QPVRMSKRAG TVITLDDLVE AIGVDAARYA LIRSSVDTPI DIDLELWSSA 430 440 450 460 470 480 SNENPVYYVQ YAHARLCALA RNAADLGVSV NTDHLDLLTH EKEGALIRNL GEFPRVLKTA 490 500 510 520 530 540 ASLREPHRVC RYLEDLAGDY HRFYDSCRVL PQGDEEPGDL HSARLALCRA TRQVIANGLA 550 ILGVSAPERM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, M. bovis bacille Calmette-Guerin (BCG) and M. tuberculosis H37Rv by allelic exchange." Pavelka M.S. Jr., Jacobs W.R. Jr. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M. Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700084 / mc(2)155.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF126720 Genomic DNA. Translation: AAD32590.1. CP000480 Genomic DNA. Translation: ABK70921.1.
RefSeq	YP_889211.1. NC_008596.1.
3D structure databases
ProteinModelPortal	Q9X5M0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9X5M0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000046341; EBMYCP00000044678; EBMYCG00000046336.
GeneID	4534266.
GenomeReviews	Gene locus MSMEG_4959 in contig CP000480_GR.
KEGG	msm:MSMEG_4959.
NMPDR	fig|246196.1.peg.4945.
PATRIC	18082217. VBIMycSme59918_4838.
TIGR	MSMEG_4959.
Phylogenomic databases
eggNOG	COG0018.
GeneTree	EBGT00050000016532.
HOGENOM	HBG695395.
OMA	FDVYFHE.
ProtClustDB	PRK01611.
Enzyme and pathway databases
BioCyc	MSME246196:MSMEG_4959-MONOMER.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ia. IPR015945. Arg-tRNA-synth_Ia_core. IPR005148. Arg-tRNA-synth_N. IPR008909. DALR_anticod-bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01887.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 1 hit. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM01016. Arg_tRNA_synt_N. 1 hit. SM00836. DALR_1. 1 hit. [Graphical view]
SUPFAM	SSF55190. Arg-tRNA-synth_Ic_N. 1 hit. SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00456. ArgS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_MYCS2

Accession

Primary (citable) accession number: Q9X5M0
Secondary accession number(s): A0R223

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	July 10, 2007
Last modified:	January 25, 2012
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program