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Q9X5M0

- SYR_MYCS2

UniProt

Q9X5M0 - SYR_MYCS2

Protein

Arginine--tRNA ligase

Gene

argS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

    GO - Molecular functioni

    1. arginine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. arginyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMSME246196:GJ4Y-4958-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arginine--tRNA ligase (EC:6.1.1.19)
    Alternative name(s):
    Arginyl-tRNA synthetase
    Short name:
    ArgRS
    Gene namesi
    Name:argS
    Ordered Locus Names:MSMEG_4959, MSMEI_4832
    OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
    Taxonomic identifieri246196 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacterium
    ProteomesiUP000000757: Chromosome, UP000006158: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 550550Arginine--tRNA ligasePRO_0000151579Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi246196.MSMEG_4959.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9X5M0.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi130 – 14011"HIGH" regionAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0018.
    HOGENOMiHOG000247214.
    KOiK01887.
    OMAiIRNTIND.
    OrthoDBiEOG6JB13C.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_00123. Arg_tRNA_synth.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PANTHERiPTHR11956. PTHR11956. 1 hit.
    PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
    PF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view]
    PRINTSiPR01038. TRNASYNTHARG.
    SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsiTIGR00456. argS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9X5M0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTPADLAELL KATAAAVLTE HDLDVAALPA TVTVERPRNP EHGDYATNLA    50
    LQLGKKVGVN PRELAGWLAT ALTAADGIAV AEVAGPGFVN LRIEASAQGV 100
    IITNVLAAEG SYGSSDQYAG RNVNLEFVSA NPTGPIHIGG TRWAAVGDAL 150
    GRLLATQGAA VTREYYFNDH GAQIDRFVNS LIASAKGEPT PEDGYAGDYI 200
    VDIAQQVIAK APDVLGLPED QQRETFRAIG VDLMFTHIKQ SLHDFGTDFD 250
    VYTHEDSMHT SGRVDQAITQ LREAGSIYEK DGAVWLRTTD FGDDKDRVVI 300
    KSDGNAAYIA GDLAYYLDKR KRGFDLCIYM LGADHHGYIA RLKAAAAALG 350
    DDPDTVEVLI GQMVNLVRDG QPVRMSKRAG TVITLDDLVE AIGVDAARYA 400
    LIRSSVDTPI DIDLELWSSA SNENPVYYVQ YAHARLCALA RNAADLGVSV 450
    NTDHLDLLTH EKEGALIRNL GEFPRVLKTA ASLREPHRVC RYLEDLAGDY 500
    HRFYDSCRVL PQGDEEPGDL HSARLALCRA TRQVIANGLA ILGVSAPERM 550
    Length:550
    Mass (Da):59,429
    Last modified:July 10, 2007 - v2
    Checksum:i4DAB7844FFFCFCEC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti149 – 1491A → T in AAD32590. 1 PublicationCurated
    Sequence conflicti232 – 2321D → N in AAD32590. 1 PublicationCurated
    Sequence conflicti255 – 2573EDS → GDW in AAD32590. 1 PublicationCurated
    Sequence conflicti331 – 3311L → V in AAD32590. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126720 Genomic DNA. Translation: AAD32590.1.
    CP000480 Genomic DNA. Translation: ABK70921.1.
    CP001663 Genomic DNA. Translation: AFP41277.1.
    RefSeqiWP_011730214.1. NC_018289.1.
    YP_006569572.1. NC_018289.1.
    YP_889211.1. NC_008596.1.

    Genome annotation databases

    EnsemblBacteriaiABK70921; ABK70921; MSMEG_4959.
    AFP41277; AFP41277; MSMEI_4832.
    GeneIDi4534266.
    KEGGimsg:MSMEI_4832.
    msm:MSMEG_4959.
    PATRICi18082217. VBIMycSme59918_4838.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF126720 Genomic DNA. Translation: AAD32590.1 .
    CP000480 Genomic DNA. Translation: ABK70921.1 .
    CP001663 Genomic DNA. Translation: AFP41277.1 .
    RefSeqi WP_011730214.1. NC_018289.1.
    YP_006569572.1. NC_018289.1.
    YP_889211.1. NC_008596.1.

    3D structure databases

    ProteinModelPortali Q9X5M0.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246196.MSMEG_4959.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABK70921 ; ABK70921 ; MSMEG_4959 .
    AFP41277 ; AFP41277 ; MSMEI_4832 .
    GeneIDi 4534266.
    KEGGi msg:MSMEI_4832.
    msm:MSMEG_4959.
    PATRICi 18082217. VBIMycSme59918_4838.

    Phylogenomic databases

    eggNOGi COG0018.
    HOGENOMi HOG000247214.
    KOi K01887.
    OMAi IRNTIND.
    OrthoDBi EOG6JB13C.

    Enzyme and pathway databases

    BioCyci MSME246196:GJ4Y-4958-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.30.1360.70. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_00123. Arg_tRNA_synth.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR001278. Arg-tRNA-ligase.
    IPR005148. Arg-tRNA-synth_N.
    IPR008909. DALR_anticod-bd.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    PANTHERi PTHR11956. PTHR11956. 1 hit.
    Pfami PF03485. Arg_tRNA_synt_N. 1 hit.
    PF05746. DALR_1. 1 hit.
    PF00750. tRNA-synt_1d. 1 hit.
    [Graphical view ]
    PRINTSi PR01038. TRNASYNTHARG.
    SMARTi SM01016. Arg_tRNA_synt_N. 1 hit.
    SM00836. DALR_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF55190. SSF55190. 1 hit.
    TIGRFAMsi TIGR00456. argS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A comparison of the construction of unmarked deletion mutations in Mycobacterium smegmatis, M. bovis bacille Calmette-Guerin (BCG) and M. tuberculosis H37Rv by allelic exchange."
      Pavelka M.S. Jr., Jacobs W.R. Jr.
      Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C., Fraser C.M.
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    3. "Interrupted coding sequences in Mycobacterium smegmatis: authentic mutations or sequencing errors?"
      Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C., Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.
      Genome Biol. 8:R20.1-R20.9(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.
    4. "Ortho-proteogenomics: multiple proteomes investigation through orthology and a new MS-based protocol."
      Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M., Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.
      Genome Res. 19:128-135(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 700084 / mc(2)155.

    Entry informationi

    Entry nameiSYR_MYCS2
    AccessioniPrimary (citable) accession number: Q9X5M0
    Secondary accession number(s): A0R223, I7G6F4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 94 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3