Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Arginine--tRNA ligase

Gene

argS

Organism
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Arginine--tRNA ligase (EC:6.1.1.19)
Alternative name(s):
Arginyl-tRNA synthetase
Short name:
ArgRS
Gene namesi
Name:argS
Ordered Locus Names:MSMEG_4959, MSMEI_4832
OrganismiMycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Taxonomic identifieri246196 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000006158 Componenti: Chromosome
  • UP000000757 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001515791 – 550Arginine--tRNA ligaseAdd BLAST550

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi246196.MSMEG_4959.

Structurei

3D structure databases

ProteinModelPortaliQ9X5M0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi130 – 140"HIGH" regionAdd BLAST11

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C75. Bacteria.
COG0018. LUCA.
HOGENOMiHOG000247214.
KOiK01887.
OMAiHVGHIRN.
OrthoDBiPOG091H01RF.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X5M0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPADLAELL KATAAAVLTE HDLDVAALPA TVTVERPRNP EHGDYATNLA
60 70 80 90 100
LQLGKKVGVN PRELAGWLAT ALTAADGIAV AEVAGPGFVN LRIEASAQGV
110 120 130 140 150
IITNVLAAEG SYGSSDQYAG RNVNLEFVSA NPTGPIHIGG TRWAAVGDAL
160 170 180 190 200
GRLLATQGAA VTREYYFNDH GAQIDRFVNS LIASAKGEPT PEDGYAGDYI
210 220 230 240 250
VDIAQQVIAK APDVLGLPED QQRETFRAIG VDLMFTHIKQ SLHDFGTDFD
260 270 280 290 300
VYTHEDSMHT SGRVDQAITQ LREAGSIYEK DGAVWLRTTD FGDDKDRVVI
310 320 330 340 350
KSDGNAAYIA GDLAYYLDKR KRGFDLCIYM LGADHHGYIA RLKAAAAALG
360 370 380 390 400
DDPDTVEVLI GQMVNLVRDG QPVRMSKRAG TVITLDDLVE AIGVDAARYA
410 420 430 440 450
LIRSSVDTPI DIDLELWSSA SNENPVYYVQ YAHARLCALA RNAADLGVSV
460 470 480 490 500
NTDHLDLLTH EKEGALIRNL GEFPRVLKTA ASLREPHRVC RYLEDLAGDY
510 520 530 540 550
HRFYDSCRVL PQGDEEPGDL HSARLALCRA TRQVIANGLA ILGVSAPERM
Length:550
Mass (Da):59,429
Last modified:July 10, 2007 - v2
Checksum:i4DAB7844FFFCFCEC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149A → T in AAD32590 (Ref. 1) Curated1
Sequence conflicti232D → N in AAD32590 (Ref. 1) Curated1
Sequence conflicti255 – 257EDS → GDW in AAD32590 (Ref. 1) Curated3
Sequence conflicti331L → V in AAD32590 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126720 Genomic DNA. Translation: AAD32590.1.
CP000480 Genomic DNA. Translation: ABK70921.1.
CP001663 Genomic DNA. Translation: AFP41277.1.
RefSeqiWP_011730214.1. NZ_CP009494.1.
YP_889211.1. NC_008596.1.

Genome annotation databases

EnsemblBacteriaiABK70921; ABK70921; MSMEG_4959.
AFP41277; AFP41277; MSMEI_4832.
GeneIDi4534266.
KEGGimsb:LJ00_24520.
msg:MSMEI_4832.
msm:MSMEG_4959.
PATRICi18082217. VBIMycSme59918_4838.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF126720 Genomic DNA. Translation: AAD32590.1.
CP000480 Genomic DNA. Translation: ABK70921.1.
CP001663 Genomic DNA. Translation: AFP41277.1.
RefSeqiWP_011730214.1. NZ_CP009494.1.
YP_889211.1. NC_008596.1.

3D structure databases

ProteinModelPortaliQ9X5M0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246196.MSMEG_4959.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABK70921; ABK70921; MSMEG_4959.
AFP41277; AFP41277; MSMEI_4832.
GeneIDi4534266.
KEGGimsb:LJ00_24520.
msg:MSMEI_4832.
msm:MSMEG_4959.
PATRICi18082217. VBIMycSme59918_4838.

Phylogenomic databases

eggNOGiENOG4105C75. Bacteria.
COG0018. LUCA.
HOGENOMiHOG000247214.
KOiK01887.
OMAiHVGHIRN.
OrthoDBiPOG091H01RF.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.30.1360.70. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_00123. Arg_tRNA_synth. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR001278. Arg-tRNA-ligase.
IPR005148. Arg-tRNA-synth_N.
IPR008909. DALR_anticod-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PANTHERiPTHR11956. PTHR11956. 1 hit.
PfamiPF03485. Arg_tRNA_synt_N. 1 hit.
PF05746. DALR_1. 1 hit.
PF00750. tRNA-synt_1d. 1 hit.
[Graphical view]
PRINTSiPR01038. TRNASYNTHARG.
SMARTiSM01016. Arg_tRNA_synt_N. 1 hit.
SM00836. DALR_1. 1 hit.
[Graphical view]
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF55190. SSF55190. 1 hit.
TIGRFAMsiTIGR00456. argS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYR_MYCS2
AccessioniPrimary (citable) accession number: Q9X5M0
Secondary accession number(s): A0R223, I7G6F4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: July 10, 2007
Last modified: November 2, 2016
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.