Reviewed, UniProtKB/Swiss-Prot Q9X5D1 (AROK_CORGL)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	Cgl1622, cg1828

Organism

Corynebacterium glutamicum (Brevibacterium flavum) [Complete proteome] [HAMAP]

Taxonomic identifier

1718 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity. HAMAP MF_00109
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00109
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity. HAMAP MF_00109
Subcellular location	Cytoplasm Probable HAMAP MF_00109.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Shikimate kinase HAMAP MF_00109		PRO_0000192376
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
Sites
	Metal binding	17	1	Magnesium By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	80	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	153	1	ATP By similarity
Experimental info
	Sequence conflict	1 – 3	3	MLL → MERNEVNDQIHLDHQSDDTS ECSC Ref.2
	Sequence conflict	1 – 3	3	MLL → MERNEVNDQIHLDHQSDDTS ECSC Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q9X5D1-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 84A16FDE5E0C1971	FASTA	169	18,382
	10 20 30 40 50 60 MLLPIVVLVG LPGAGKSTIG RRLARALNTE LVDSDELIER ATGKACGAVF SELGEPAFRE 70 80 90 100 110 120 LEAIHVAEAL KSSGVVSLGG GSVLTESTRE LLKGQDVVWI DVPVEEGIRR TANERSRPVL 130 140 150 160 QAADPAEHYR NLVKVRTPLY EEVATYRLRT NNRSPQQVVA AVLHHLEID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genetic aspects of the prechorismate pathway in Corynebacterium glutamicum." Burke K.G., Joy J., O'Donohue M.R., Dunican L.K. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"Complete genomic sequence of Corynebacterium glutamicum ATCC 13032." Nakagawa S. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed: 12948626] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF124600 Genomic DNA. Translation: AAD27839.1. BA000036 Genomic DNA. Translation: BAB99015.1. BX927152 Genomic DNA. Translation: CAF21631.1. Different initiation.
RefSeq	NP_600836.1. YP_225907.1.
3D structure databases
SMR	Q9X5D1. Positions 4-165.
ModBase	Search...
Genome annotation databases
GeneID	1019591. 3343455.
GenomeReviews	Gene locus Cgl1622 in contig BA000036_GR.
KEGG	cgb:cg1828. cgl:NCgl1560.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335815.
OMA	HANVFFV.
PhylomeDB	Q9X5D1.
Enzyme and pathway databases
BioCyc	CGLU196627:CG1828-MONOMER.
BRENDA	2.7.1.71. 812.
Family and domain databases
HAMAP	MF_00109. Shikimate_kinase. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_CORGL

Accession

Primary (citable) accession number: Q9X5D1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)