Q9X528 (RNPH_LEGPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease PH
Short name=RNase PH
EC=2.7.7.56

Alternative name(s):
tRNA nucleotidyltransferase

Gene names

Name:

rph

Organism

Legionella pneumophila

Taxonomic identifier

446 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Legionellales › Legionellaceae › Legionella

Protein attributes

Sequence length	235 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates By similarity. HAMAP-Rule MF_00564
Catalytic activity	tRNA(n+1) + phosphate = tRNA(n) + a nucleoside diphosphate. HAMAP-Rule MF_00564
Sequence similarities	Belongs to the RNase PH family.

Ontologies

Keywords
Biological process	tRNA processing
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological_process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular_function	tRNA binding Inferred from electronic annotation. Source: HAMAP tRNA nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA-specific ribonuclease activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 235	235	Ribonuclease PH HAMAP-Rule MF_00564		PRO_0000139902

Sequences

	Sequence		Length	Mass (Da)
	Q9X528 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B139F9AE430050CE	FASTA	235	26,110
	10 20 30 40 50 60 MRPSNREHDQ LRPVTITRNF TNYAEGSVLV EFGQTKVICN ASIVEGVPRF LKGKNQGWIT 70 80 90 100 110 120 AEYGMLPRAT HSRTEREASK GKQGGRTLEI QRLIGRSLRA CIDLKVLGEN TITLDCDVIQ 130 140 150 160 170 180 ADGGTRTAAI TGSCVAMRDA IHWMVQREKI KKMPAFNYVA AVSVGIYRGQ PVLDLDYAED 190 200 210 220 230 VLAETDMNVV MNEQGHFIEV QGTAEDNSFN REQLNSMLSL AEIGIPQLIE IQKNA

References

[1]	"Cloning and sequencing of the rph gene encoding RNase PH from Legionella pneumophila." Kim S.J., Choe Y.K. Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 130b / Wadsworth / Serogroup 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF120720 Genomic DNA. Translation: AAD28218.1.
3D structure databases
ProteinModelPortal	Q9X528.
SMR	Q9X528. Positions 2-235.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00564. RNase_PH.
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR020568. Ribosomal_S5_D2-typ_fold. IPR002381. RNase_PH_bac-type. IPR018336. RNase_PH_CS. [Graphical view]
Pfam	PF01138. RNase_PH. 1 hit. PF03725. RNase_PH_C. 1 hit. [Graphical view]
SUPFAM	SSF55666. 3_ExoRNase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR01966. RNasePH. 1 hit.
PROSITE	PS01277. RIBONUCLEASE_PH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPH_LEGPN

Accession

Primary (citable) accession number: Q9X528

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2002
Last sequence update:	November 1, 1999
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program