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Reviewed, UniProtKB/Swiss-Prot Q9X4Y0 (AGAL_RHIME)

Last modified January 19, 2010. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Melibiase
Gene names
Name: melA
Ordered Locus Names: RB1568
ORF Names: SMb21648
Encoded onPlasmid pSymB (megaplasmid 2)
OrganismRhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier382 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Binds 1 NAD per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 4 family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
   LigandManganese
Metal-binding
NAD
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Plasmid
Gene Ontology (GO)
   Biological processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalpha-galactosidase activity

Inferred from electronic annotation. Source: EC

manganese ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Alpha-galactosidase
PRO_0000169853

Regions

Nucleotide binding4 – 7067NAD By similarity

Sites

Active site1721Proton donor By similarity
Active site2581Proton acceptor By similarity
Metal binding1711Manganese By similarity
Metal binding2011Manganese By similarity
Binding site1501Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X4Y0-1 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B68698CF901F6BCD

FASTA49054,605
        10         20         30         40         50         60 
MASFKIAIIG AGSIGFTKKL FTDILSVPEL RDVEFALTDL SEHNLAMIKS ILDRIVEANE 

        70         80         90        100        110        120 
LPTRVTATTD RRKALEGARY IISCVRVGGL EAYADDIRIP LKYGVDQCVG DTICAGGILY 

       130        140        150        160        170        180 
GQRNIPVILD FCKDIREVAE PGAKFLNYAN PMAMNTWAAI EYGKVDTVGL CHGVQHGAEQ 

       190        200        210        220        230        240 
IAEILGARDG ELDYICSGIN HQTWFVDVRL NGRKVGKDEL VAAFEAHPVF SKQEKLRIDV 

       250        260        270        280        290        300 
LKRFGVYSTE SNGHLSEYLP WYRKRPDEIS RWIDMSDWIH GETGGYLRYS TETRNWFETE 

       310        320        330        340        350        360 
YPRFLEEAGR PLETIRRSNE HASRILEALE TGRVYRGHFN VKNNGVITNL PADAIIESPG 

       370        380        390        400        410        420 
FVDRFGINMV AGITLPEACA ATCISSVNVQ RMSVHAAITG DIDLLKLAVL HDPLVGAICT 

       430        440        450        460        470        480 
PEEVWQMVDE MVVAQAKWLP QYAHAIDAAK ERLARATVAT REWKGAARRE VRSIEEIRAE 

       490 
KEAAKLRAAG 

« Hide

References

« Hide 'large scale' references
[1]"Alpha-galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization."
Gage D.J., Long S.R.
J. Bacteriol. 180:5739-5748(1998) [PubMed: 9791127] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1021.
[2]"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF119834 Genomic DNA. Translation: AAD26273.1.
AL591985 Genomic DNA. Translation: CAC49967.1.
PIRG96037.
RefSeqNP_438107.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyGH4. Glycoside Hydrolase Family 4.

Genome annotation databases

GeneID1237899.
GenomeReviewsGene locus RB1568 in contig AL591985_GR.
KEGGsme:SM_b21648.
NMPDRfig|266834.1.peg.6202.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG511715.
OMATICAGGI.

Enzyme and pathway databases

BioCycSMEL266834:SMB21648-MONOMER.
BRENDA3.2.1.22. 142.

Family and domain databases

InterProIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
PfamPF02056. Glyco_hydro_4. 1 hit.
[Graphical view]
PRINTSPR00732. GLHYDRLASE4.
PROSITEPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGAL_RHIME
AccessionPrimary (citable) accession number: Q9X4Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents