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Q9X4Y0 (AGAL_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-galactosidase
EC=3.2.1.22
Alternative name(s):
Melibiase
Gene names
Name:melA
Ordered Locus Names:RB1568
ORF Names:SMb21648
Encoded onPlasmid pSymB (megaplasmid 2)
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length490 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Cofactor

Binds 1 manganese ion per subunit By similarity.

Binds 1 NAD per subunit By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the glycosyl hydrolase 4 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 490490Alpha-galactosidase
PRO_0000169853

Regions

Nucleotide binding4 – 7067NAD By similarity

Sites

Active site1721Proton donor By similarity
Active site2581Proton acceptor By similarity
Metal binding1711Manganese By similarity
Metal binding2011Manganese By similarity
Binding site1501Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X4Y0 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: B68698CF901F6BCD

FASTA49054,605
        10         20         30         40         50         60 
MASFKIAIIG AGSIGFTKKL FTDILSVPEL RDVEFALTDL SEHNLAMIKS ILDRIVEANE 

        70         80         90        100        110        120 
LPTRVTATTD RRKALEGARY IISCVRVGGL EAYADDIRIP LKYGVDQCVG DTICAGGILY 

       130        140        150        160        170        180 
GQRNIPVILD FCKDIREVAE PGAKFLNYAN PMAMNTWAAI EYGKVDTVGL CHGVQHGAEQ 

       190        200        210        220        230        240 
IAEILGARDG ELDYICSGIN HQTWFVDVRL NGRKVGKDEL VAAFEAHPVF SKQEKLRIDV 

       250        260        270        280        290        300 
LKRFGVYSTE SNGHLSEYLP WYRKRPDEIS RWIDMSDWIH GETGGYLRYS TETRNWFETE 

       310        320        330        340        350        360 
YPRFLEEAGR PLETIRRSNE HASRILEALE TGRVYRGHFN VKNNGVITNL PADAIIESPG 

       370        380        390        400        410        420 
FVDRFGINMV AGITLPEACA ATCISSVNVQ RMSVHAAITG DIDLLKLAVL HDPLVGAICT 

       430        440        450        460        470        480 
PEEVWQMVDE MVVAQAKWLP QYAHAIDAAK ERLARATVAT REWKGAARRE VRSIEEIRAE 

       490 
KEAAKLRAAG

« Hide

References

« Hide 'large scale' references
[1]"Alpha-galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization."
Gage D.J., Long S.R.
J. Bacteriol. 180:5739-5748(1998) [PubMed: 9791127] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1021.
[2]"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti."
Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.
Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed: 11474104] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF119834 Genomic DNA. Translation: AAD26273.1.
AL591985 Genomic DNA. Translation: CAC49967.1.
PIRG96037.
RefSeqNP_438107.1. NC_003078.1.

3D structure databases

ProteinModelPortalQ9X4Y0.
ModBaseSearch...

Protein family/group databases

CAZyGH4. Glycoside Hydrolase Family 4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1237899.
GenomeReviewsGene locus RB1568 in contig AL591985_GR.
KEGGsme:SM_b21648.
NMPDRfig|266834.1.peg.6202.
PATRIC23639548. VBISinMel96828_6494.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG511715.
OMATICAGGI.
ProtClustDBPRK15076.

Enzyme and pathway databases

BioCycSMEL266834:SMB21648-MONOMER.

Family and domain databases

InterProIPR019802. GlycHydrolase_4_CS.
IPR001088. Glyco_hydro_4.
IPR022616. Glyco_hydro_4_C.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KOK07406.
PfamPF02056. Glyco_hydro_4. 1 hit.
PF11975. Glyco_hydro_4C. 1 hit.
[Graphical view]
PRINTSPR00732. GLHYDRLASE4.
SUPFAMSSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
PROSITEPS01324. GLYCOSYL_HYDROL_F4. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGAL_RHIME
AccessionPrimary (citable) accession number: Q9X4Y0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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