Q9X4Y0 (AGAL_RHIME) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 80.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-galactosidase EC=3.2.1.22 Alternative name(s): Melibiase | ||||||
| Gene names |
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| Encoded on | Plasmid pSymB (megaplasmid 2) | ||||||
| Organism | Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP] | ||||||
| Taxonomic identifier | 266834 [NCBI] | ||||||
| Taxonomic lineage | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium |
Protein attributes
| Sequence length | 490 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Catalytic activity | Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. |
| Cofactor | Binds 1 manganese ion per subunit By similarity. Binds 1 NAD per subunit By similarity. |
| Subunit structure | Homodimer By similarity. |
| Sequence similarities | Belongs to the glycosyl hydrolase 4 family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Carbohydrate metabolism |
| Ligand | Manganese Metal-binding NAD |
| Molecular function | Glycosidase Hydrolase |
| Technical term | Complete proteome Plasmid |
| Gene Ontology (GO) | |
| Biological process | carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide bindingInferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptorInferred from electronic annotation. Source: InterPro raffinose alpha-galactosidase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 490 | 490 | Alpha-galactosidase | PRO_0000169853 | |||||
Regions | |||||||||
| Nucleotide binding | 4 – 70 | 67 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 172 | 1 | Proton donor By similarity | ||||||
| Active site | 258 | 1 | Proton acceptor By similarity | ||||||
| Metal binding | 171 | 1 | Manganese By similarity | ||||||
| Metal binding | 201 | 1 | Manganese By similarity | ||||||
| Binding site | 150 | 1 | Substrate By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Alpha-galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization." Gage D.J., Long S.R. J. Bacteriol. 180:5739-5748(1998) [PubMed: 9791127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021. |
| [2] | "The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021. |
| [3] | "The composite genome of the legume symbiont Sinorhizobium meliloti." Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. Batut J.Science 293:668-672(2001) [PubMed: 11474104] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AF119834 Genomic DNA. Translation: AAD26273.1. AL591985 Genomic DNA. Translation: CAC49967.1. |
| PIR | G96037. |
| RefSeq | NP_438107.1. NC_003078.1. |
3D structure databases | |
| ProteinModelPortal | Q9X4Y0. |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GH4. Glycoside Hydrolase Family 4. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 1237899. |
| GenomeReviews | Gene locus RB1568 in contig AL591985_GR. |
| KEGG | sme:SM_b21648. |
| NMPDR | fig|266834.1.peg.6202. |
| PATRIC | 23639548. VBISinMel96828_6494. |
Organism-specific databases | |
| CMR | Search... |
Phylogenomic databases | |
| HOGENOM | HBG511715. |
| OMA | TICAGGI. |
| ProtClustDB | PRK15076. |
Enzyme and pathway databases | |
| BioCyc | SMEL266834:SMB21648-MONOMER. |
Family and domain databases | |
| InterPro | IPR019802. GlycHydrolase_4_CS. IPR001088. Glyco_hydro_4. IPR022616. Glyco_hydro_4_C. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| KO | K07406. |
| Pfam | PF02056. Glyco_hydro_4. 1 hit. PF11975. Glyco_hydro_4C. 1 hit. [Graphical view] |
| PRINTS | PR00732. GLHYDRLASE4. |
| SUPFAM | SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit. |
| PROSITE | PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | AGAL_RHIME | ||||||||
| Accession | Primary (citable) accession number: Q9X4Y0 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Glycosyl hydrolases Classification of glycosyl hydrolase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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