Reviewed, UniProtKB/Swiss-Prot Q9X4Y0 (AGAL_RHIME)

Names and origin

Protein names

Recommended name:
    Alpha-galactosidase
    EC=3.2.1.22
Alternative name(s):
    Melibiase

Gene names

Name:	melA
Ordered Locus Names:	RB1568
ORF Names:	SMb21648

Encoded on

Plasmid pSymB (megaplasmid 2)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	490 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Cofactor	Binds 1 manganese ion per subunit By similarity. Binds 1 NAD per subunit By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 4 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	Manganese Metal-binding NAD
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-galactosidase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 490	490	Alpha-galactosidase		PRO_0000169853
Regions
	Nucleotide binding	4 – 70	67	NAD By similarity
Sites
	Active site	172	1	Proton donor By similarity
	Active site	258	1	Proton acceptor By similarity
	Metal binding	171	1	Manganese By similarity
	Metal binding	201	1	Manganese By similarity
	Binding site	150	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X4Y0-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: B68698CF901F6BCD	FASTA	490	54,605
	10 20 30 40 50 60 MASFKIAIIG AGSIGFTKKL FTDILSVPEL RDVEFALTDL SEHNLAMIKS ILDRIVEANE 70 80 90 100 110 120 LPTRVTATTD RRKALEGARY IISCVRVGGL EAYADDIRIP LKYGVDQCVG DTICAGGILY 130 140 150 160 170 180 GQRNIPVILD FCKDIREVAE PGAKFLNYAN PMAMNTWAAI EYGKVDTVGL CHGVQHGAEQ 190 200 210 220 230 240 IAEILGARDG ELDYICSGIN HQTWFVDVRL NGRKVGKDEL VAAFEAHPVF SKQEKLRIDV 250 260 270 280 290 300 LKRFGVYSTE SNGHLSEYLP WYRKRPDEIS RWIDMSDWIH GETGGYLRYS TETRNWFETE 310 320 330 340 350 360 YPRFLEEAGR PLETIRRSNE HASRILEALE TGRVYRGHFN VKNNGVITNL PADAIIESPG 370 380 390 400 410 420 FVDRFGINMV AGITLPEACA ATCISSVNVQ RMSVHAAITG DIDLLKLAVL HDPLVGAICT 430 440 450 460 470 480 PEEVWQMVDE MVVAQAKWLP QYAHAIDAAK ERLARATVAT REWKGAARRE VRSIEEIRAE 490 KEAAKLRAAG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alpha-galactoside uptake in Rhizobium meliloti: isolation and characterization of agpA, a gene encoding a periplasmic binding protein required for melibiose and raffinose utilization." Gage D.J., Long S.R. J. Bacteriol. 180:5739-5748(1998) [PubMed: 9791127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1021.
[2]	"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti." Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A. Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001) [PubMed: 11481431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF119834 Genomic DNA. Translation: AAD26273.1. AL591985 Genomic DNA. Translation: CAC49967.1.
PIR	G96037.
RefSeq	NP_438107.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH4. Glycoside Hydrolase Family 4.
Genome annotation databases
GeneID	1237899.
GenomeReviews	Gene locus RB1568 in contig AL591985_GR.
KEGG	sme:SM_b21648.
NMPDR	fig|266834.1.peg.6202.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG511715.
OMA	TICAGGI.
Enzyme and pathway databases
BioCyc	SMEL266834:SMB21648-MONOMER.
BRENDA	3.2.1.22. 142.
Family and domain databases
InterPro	IPR019802. GlycHydrolase_4_CS. IPR001088. Glyco_hydro_4. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit.
Pfam	PF02056. Glyco_hydro_4. 1 hit. [Graphical view]
PRINTS	PR00732. GLHYDRLASE4.
PROSITE	PS01324. GLYCOSYL_HYDROL_F4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGAL_RHIME

Accession

Primary (citable) accession number: Q9X4Y0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)