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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi70 – 701Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi76 – 761Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi79 – 791Iron-sulfur (2Fe-2S)UniRule annotation
Metal bindingi111 – 1111Iron-sulfur (2Fe-2S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16202.
VCHO:VC2290-MONOMER.
BRENDAi1.6.5.8. 6626.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Ordered Locus Names:VC_2290
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei4 – 2421HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408Na(+)-translocating NADH-quinone reductase subunit FPRO_0000074506Add
BLAST

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

DIPiDIP-61342N.
STRINGi243277.VC2290.

Structurei

Secondary structure

1
408
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 2624Combined sources
Turni27 – 293Combined sources
Beta strandi37 – 437Combined sources
Beta strandi45 – 473Combined sources
Helixi55 – 628Combined sources
Beta strandi70 – 745Combined sources
Helixi92 – 976Combined sources
Beta strandi99 – 1013Combined sources
Turni110 – 1123Combined sources
Beta strandi113 – 1153Combined sources
Beta strandi131 – 14414Combined sources
Beta strandi147 – 1537Combined sources
Helixi155 – 1573Combined sources
Beta strandi167 – 1726Combined sources
Beta strandi174 – 1785Combined sources
Helixi179 – 1813Combined sources
Helixi186 – 1883Combined sources
Helixi190 – 1945Combined sources
Helixi197 – 1993Combined sources
Beta strandi201 – 2044Combined sources
Beta strandi208 – 2147Combined sources
Beta strandi223 – 2297Combined sources
Helixi244 – 2518Combined sources
Beta strandi257 – 2648Combined sources
Beta strandi276 – 2816Combined sources
Helixi282 – 2843Combined sources
Helixi285 – 29713Combined sources
Beta strandi305 – 3139Combined sources
Helixi314 – 3163Combined sources
Helixi320 – 32910Combined sources
Beta strandi333 – 3419Combined sources
Helixi344 – 3463Combined sources
Beta strandi350 – 3534Combined sources
Helixi355 – 3628Combined sources
Turni363 – 3664Combined sources
Helixi370 – 3723Combined sources
Beta strandi373 – 3786Combined sources
Helixi381 – 39313Combined sources
Helixi398 – 4003Combined sources
Beta strandi401 – 4033Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50F1-408[»]
4U9UX-ray1.55A/B130-408[»]
ProteinModelPortaliQ9X4Q8.
SMRiQ9X4Q8. Positions 129-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 127952Fe-2S ferredoxin-typeUniRule annotationAdd
BLAST
Domaini130 – 270141FAD-binding FR-typeUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni273 – 390118CatalyticAdd
BLAST

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.
OrthoDBiEOG6JHRM2.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X4Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIIFGVVM FTLIILALVL VILFAKSKLV PTGDITISIN GDPEKAIVTQ
60 70 80 90 100
PGGKLLTALA GAGVFVSSAC GGGGSCGQCR VKIKSGGGDI LPTELDHISK
110 120 130 140 150
GEAREGERLA CQVAVKADMD LELPEEIFGV KKWECTVISN DNKATFIKEL
160 170 180 190 200
KLAIPDGESV PFRAGGYIQI EAPAHHVKYA DFDVPEKYRG DWDKFNLFRY
210 220 230 240 250
ESKVDEPIIR AYSMANYPEE FGIIMLNVRI ATPPPNNPNV PPGQMSSYIW
260 270 280 290 300
SLKAGDKCTI SGPFGEFFAK DTDAEMVFIG GGAGMAPMRS HIFDQLKRLK
310 320 330 340 350
SKRKMSYWYG ARSKREMFYV EDFDGLAAEN DNFVWHCALS DPQPEDNWTG
360 370 380 390 400
YTGFIHNVLY ENYLKDHEAP EDCEYYMCGP PMMNAAVINM LKNLGVEEEN

ILLDDFGG
Length:408
Mass (Da):45,067
Last modified:November 1, 1999 - v1
Checksum:iF5665E9623CAAD7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29967.1.
AE003852 Genomic DNA. Translation: AAF95434.1.
PIRiB82094.
RefSeqiNP_231921.1. NC_002505.1.
WP_000103964.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95434; AAF95434; VC_2290.
GeneIDi2613212.
KEGGivch:VC2290.
PATRICi20083619. VBIVibCho83274_2184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29967.1.
AE003852 Genomic DNA. Translation: AAF95434.1.
PIRiB82094.
RefSeqiNP_231921.1. NC_002505.1.
WP_000103964.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50F1-408[»]
4U9UX-ray1.55A/B130-408[»]
ProteinModelPortaliQ9X4Q8.
SMRiQ9X4Q8. Positions 129-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61342N.
STRINGi243277.VC2290.

Protocols and materials databases

DNASUi2613212.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95434; AAF95434; VC_2290.
GeneIDi2613212.
KEGGivch:VC2290.
PATRICi20083619. VBIVibCho83274_2184.

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.
OrthoDBiEOG6JHRM2.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16202.
VCHO:VC2290-MONOMER.
BRENDAi1.6.5.8. 6626.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Effects of changes in membrane sodium flux on virulence gene expression in Vibrio cholerae."
    Haese C.C., Mekalanos J.J.
    Proc. Natl. Acad. Sci. U.S.A. 96:3183-3187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 39315 / El Tor Inaba N16961.

Entry informationi

Entry nameiNQRF_VIBCH
AccessioniPrimary (citable) accession number: Q9X4Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1999
Last modified: November 11, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.