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Protein

Na(+)-translocating NADH-quinone reductase subunit F

Gene

nqrF

Organism
Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na+ ions from the cytoplasm to the periplasm. The first step is catalyzed by NqrF, which accepts electrons from NADH and reduces ubiquinone-1 to ubisemiquinone by a one-electron transfer pathway.UniRule annotation

Catalytic activityi

NADH + ubiquinone + n Na+(In) = NAD+ + ubiquinol + n Na+(Out).UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster.UniRule annotation
  • FADUniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi76Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi79Iron-sulfur (2Fe-2S)UniRule annotation1
Metal bindingi111Iron-sulfur (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, NAD, Sodium, Ubiquinone

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16202.
VCHO:VC2290-MONOMER.
BRENDAi1.6.5.8. 6626.

Names & Taxonomyi

Protein namesi
Recommended name:
Na(+)-translocating NADH-quinone reductase subunit FUniRule annotation (EC:1.6.5.8UniRule annotation)
Short name:
Na(+)-NQR subunit FUniRule annotation
Short name:
Na(+)-translocating NQR subunit FUniRule annotation
Alternative name(s):
NQR complex subunit FUniRule annotation
NQR-1 subunit FUniRule annotation
Gene namesi
Name:nqrFUniRule annotation
Ordered Locus Names:VC_2290
OrganismiVibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
Taxonomic identifieri243277 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaVibrionalesVibrionaceaeVibrio
Proteomesi
  • UP000000584 Componenti: Chromosome 1

Subcellular locationi

  • Cell inner membrane UniRule annotation; Single-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei4 – 24HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000745061 – 408Na(+)-translocating NADH-quinone reductase subunit FAdd BLAST408

Interactioni

Subunit structurei

Composed of six subunits; NqrA, NqrB, NqrC, NqrD, NqrE and NqrF.UniRule annotation

Protein-protein interaction databases

DIPiDIP-61342N.
STRINGi243277.VC2290.

Structurei

Secondary structure

1408
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 26Combined sources24
Turni27 – 29Combined sources3
Beta strandi37 – 43Combined sources7
Beta strandi45 – 47Combined sources3
Helixi55 – 62Combined sources8
Beta strandi70 – 74Combined sources5
Helixi92 – 97Combined sources6
Beta strandi99 – 101Combined sources3
Turni110 – 112Combined sources3
Beta strandi113 – 115Combined sources3
Beta strandi131 – 144Combined sources14
Beta strandi147 – 153Combined sources7
Helixi155 – 157Combined sources3
Beta strandi167 – 172Combined sources6
Beta strandi174 – 178Combined sources5
Helixi179 – 181Combined sources3
Helixi186 – 188Combined sources3
Helixi190 – 194Combined sources5
Helixi197 – 199Combined sources3
Beta strandi201 – 204Combined sources4
Beta strandi208 – 214Combined sources7
Beta strandi223 – 229Combined sources7
Helixi244 – 251Combined sources8
Beta strandi257 – 264Combined sources8
Beta strandi276 – 281Combined sources6
Helixi282 – 284Combined sources3
Helixi285 – 297Combined sources13
Beta strandi305 – 313Combined sources9
Helixi314 – 316Combined sources3
Helixi320 – 329Combined sources10
Beta strandi333 – 341Combined sources9
Helixi344 – 346Combined sources3
Beta strandi350 – 353Combined sources4
Helixi355 – 362Combined sources8
Turni363 – 366Combined sources4
Helixi370 – 372Combined sources3
Beta strandi373 – 378Combined sources6
Helixi381 – 393Combined sources13
Helixi398 – 400Combined sources3
Beta strandi401 – 403Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50F1-408[»]
4U9UX-ray1.55A/B130-408[»]
ProteinModelPortaliQ9X4Q8.
SMRiQ9X4Q8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 1272Fe-2S ferredoxin-typeUniRule annotationAdd BLAST95
Domaini130 – 270FAD-binding FR-typeUniRule annotationAdd BLAST141

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni273 – 390CatalyticAdd BLAST118

Sequence similaritiesi

Belongs to the NqrF family.UniRule annotation
Contains 1 2Fe-2S ferredoxin-type domain.UniRule annotation
Contains 1 FAD-binding FR-type domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X4Q8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTIIFGVVM FTLIILALVL VILFAKSKLV PTGDITISIN GDPEKAIVTQ
60 70 80 90 100
PGGKLLTALA GAGVFVSSAC GGGGSCGQCR VKIKSGGGDI LPTELDHISK
110 120 130 140 150
GEAREGERLA CQVAVKADMD LELPEEIFGV KKWECTVISN DNKATFIKEL
160 170 180 190 200
KLAIPDGESV PFRAGGYIQI EAPAHHVKYA DFDVPEKYRG DWDKFNLFRY
210 220 230 240 250
ESKVDEPIIR AYSMANYPEE FGIIMLNVRI ATPPPNNPNV PPGQMSSYIW
260 270 280 290 300
SLKAGDKCTI SGPFGEFFAK DTDAEMVFIG GGAGMAPMRS HIFDQLKRLK
310 320 330 340 350
SKRKMSYWYG ARSKREMFYV EDFDGLAAEN DNFVWHCALS DPQPEDNWTG
360 370 380 390 400
YTGFIHNVLY ENYLKDHEAP EDCEYYMCGP PMMNAAVINM LKNLGVEEEN

ILLDDFGG
Length:408
Mass (Da):45,067
Last modified:November 1, 1999 - v1
Checksum:iF5665E9623CAAD7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29967.1.
AE003852 Genomic DNA. Translation: AAF95434.1.
PIRiB82094.
RefSeqiNP_231921.1. NC_002505.1.
WP_000103964.1. NC_002505.1.

Genome annotation databases

EnsemblBacteriaiAAF95434; AAF95434; VC_2290.
GeneIDi2613212.
KEGGivch:VC2290.
PATRICi20083619. VBIVibCho83274_2184.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF117331 Genomic DNA. Translation: AAD29967.1.
AE003852 Genomic DNA. Translation: AAF95434.1.
PIRiB82094.
RefSeqiNP_231921.1. NC_002505.1.
WP_000103964.1. NC_002505.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4P6VX-ray3.50F1-408[»]
4U9UX-ray1.55A/B130-408[»]
ProteinModelPortaliQ9X4Q8.
SMRiQ9X4Q8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-61342N.
STRINGi243277.VC2290.

Protocols and materials databases

DNASUi2613212.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF95434; AAF95434; VC_2290.
GeneIDi2613212.
KEGGivch:VC2290.
PATRICi20083619. VBIVibCho83274_2184.

Phylogenomic databases

eggNOGiENOG4105D26. Bacteria.
COG2871. LUCA.
KOiK00351.
OMAiEEHGIIM.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16202.
VCHO:VC2290-MONOMER.
BRENDAi1.6.5.8. 6626.

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
HAMAPiMF_00430. NqrF. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR017927. Fd_Rdtase_FAD-bd.
IPR010205. NqrF.
IPR008333. OxRdtase_FAD-bd_dom.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00970. FAD_binding_6. 1 hit.
PF00111. Fer2. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000044. Cis_Diol_DH_RD. 1 hit.
SUPFAMiSSF54292. SSF54292. 1 hit.
SSF63380. SSF63380. 1 hit.
TIGRFAMsiTIGR01941. nqrF. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNQRF_VIBCH
AccessioniPrimary (citable) accession number: Q9X4Q8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: November 1, 1999
Last modified: November 2, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.