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Q9X4K8 (MDH_GEOTD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
OrganismGeobacillus thermodenitrificans
Taxonomic identifier33940 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   PTMPhosphoprotein
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113435

Regions

Nucleotide binding12 – 176NAD By similarity
Nucleotide binding123 – 1253NAD By similarity

Sites

Active site1801Proton acceptor By similarity
Binding site361NAD By similarity
Binding site871Substrate By similarity
Binding site931Substrate By similarity
Binding site1001NAD By similarity
Binding site1251Substrate By similarity
Binding site1561Substrate By similarity

Amino acid modifications

Modified residue1491Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9X4K8 [UniParc].

Last modified November 1, 1999. Version 1.
Checksum: 68C691C462EFF452

FASTA31233,682
        10         20         30         40         50         60 
MAMKRKKISV IGAGFTGATT AFLLAQKELG DIVLVDIPQL ENPTKGKALD MLESSPVLGF 

        70         80         90        100        110        120 
DANIVGTSDY ADTADSDIVV ITAGIARKPG MSRDDLVTTN QKIMKQVTKE VVKYSPNCYI 

       130        140        150        160        170        180 
IVLTNPVDAM SYTVFKESGF PKNRVIGQSG VLDTARFRTF VAQELNISVK DVTGFVLGGH 

       190        200        210        220        230        240 
GDDMVPLVRY SYAGGIPLEK LIPKDRLDAI VERTRKGGGE IVNLLGNGSA YYAPAASLAE 

       250        260        270        280        290        300 
MVEAIVKDQR RILPAITYLE GEYGYEGIYL GVPTILGGNG IEKVIELELT EDEKAALAKS 

       310 
LESVKNVMRV LE 

« Hide

References

[1]"Properties and primary structure of a thermostable L-malate dehydrogenase from 'Bacillus thermodenitrificans'."
Williams R.A.D., Welch S.G., Alawadhi S.A.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 00462.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF114423 Genomic DNA. Translation: AAD28555.1.

3D structure databases

ProteinModelPortalQ9X4K8.
SMRQ9X4K8. Positions 5-308.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01763. MalateDH_bact. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_GEOTD
AccessionPrimary (citable) accession number: Q9X4K8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 1, 1999
Last modified: February 19, 2014
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families