Reviewed, UniProtKB/Swiss-Prot Q9X4H1 (SERC_EDWI9)

Names and origin

Protein names

Recommended name:
    Phosphoserine aminotransferase
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase
      Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	NT01EI_2459

Organism

Edwardsiella ictaluri (strain 93-146) [Complete proteome] [HAMAP]

Taxonomic identifier

634503 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Edwardsiella

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160 Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5. HAMAP MF_00160
Subunit structure	Homodimer By similarity. HAMAP MF_00160
Subcellular location	Cytoplasm By similarity HAMAP MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Pyridoxine biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: HAMAP pyridoxine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Phosphoserine aminotransferase HAMAP MF_00160		PRO_0000150170
Regions
	Region	76 – 77	2	Pyridoxal phosphate binding By similarity
	Region	240 – 241	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	42	1	L-glutamate By similarity
	Binding site	104	1	Pyridoxal phosphate By similarity
	Binding site	155	1	Pyridoxal phosphate By similarity
	Binding site	175	1	Pyridoxal phosphate By similarity
	Binding site	198	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	199	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X4H1-1 [UniParc]. Last modified September 1, 2009. Version 2. Checksum: 945229E9EE3F65FB	FASTA	363	40,210
	10 20 30 40 50 60 MTQVYNFCAG PAMLPVEVLR RAEQELCNWH GLGTSVMEIS HRGEAFMQVA AEAEQDLRDL 70 80 90 100 110 120 LKVPADYRIL FCHGGARGQF AAVPLNLLGS ERTARADYAV GGYWAQSAAK EAQRYCNPHA 130 140 150 160 170 180 IDIAQRVDGK LSMKPMAQWD LLEESAYLHY CPNETIDGVT IDEEPDFGDR VVVADMSSTI 190 200 210 220 230 240 LSRPIDVSRY GLIYAGAQKN VGPAGITLVI VREDLLGRAR REIPSILDYQ VLVESDSMFN 250 260 270 280 290 300 TPPTFAWYLS GMVFKWLKEQ GGVSEMARRN QAKADLLYQA IDGGEFYRND VAPANRSRMN 310 320 330 340 350 360 VPFQMVDSAL DPLFWDEARK AGLHALKGHK VAGGMRASIY NAMPLAGVQA LVAFMADFAR RHG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Edwardsiella ictaluri 93-146." Williams M.L., Gillaspy A.F., Dyer D.W., Thune R.L., Waldbieser G.C., Schuster S.C., Gipson J., Zaitshik J., Landry C., Lawrence M.L. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"An aroA mutant of Edwardsiella ictaluri is safe and efficacious as a live, attenuated vaccine." Thune R.L., Fernandez D.H., Battista J.R. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 313-363.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001600 Genomic DNA. Translation: ACR69629.1. AF110153 Genomic DNA. Translation: AAD28374.1.
RefSeq	YP_002933864.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	7959191.
GenomeReviews	Gene locus NT01EI_2459 in contig CP001600_GR.
KEGG	eic:NT01EI_2459.
Organism-specific databases
CMR	Search...
Enzyme and pathway databases
BRENDA	2.6.1.52. 279047.
Family and domain databases
HAMAP	MF_00160. SerC_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_EDWI9

Accession

Primary (citable) accession number: Q9X4H1
Secondary accession number(s): C5BBS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	September 1, 2009
Last modified:	February 9, 2010
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)