Q9X4G0 (HGD_PSEAE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	PA2009

Organism

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228) [Reference proteome] [HAMAP]

Taxonomic identifier

208964 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas aeruginosa group › Pseudomonas aeruginosa

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_0000220249
Sites
	Metal binding	330	1	Iron By similarity
	Metal binding	336	1	Iron By similarity
	Metal binding	366	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9X4G0 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 44B17EC2A0811E2A	FASTA	432	47,841
	10 20 30 40 50 60 MNLDSTALAY QSGFGNEFSS EALPGALPVG QNSPQKAPYG LYAELLSGTA FTMARSEARR 70 80 90 100 110 120 TWLYRITPSA KHPPFRRLER QIAGAELDAP TPNRLRWDPL ALPEQPTDFL DGLLRMAANA 130 140 150 160 170 180 PGDKPAGVSI YQYLANRSME RCFYDADGEL LLVPQLGRLR LCTELGALQV EPLEIAVIPR 190 200 210 220 230 240 GMKFRVELLD GEARGYIAEN HGAPLRLPDL GPIGSNGLAN PRDFLTPVAR YEDSRQPLQL 250 260 270 280 290 300 VQKYLGELWA CELDHSPLDV VAWHGNNVPY KYDLRRFNTI GTVSFDHPDP SIFTVLTSPT 310 320 330 340 350 360 SVPGLANIDF VIFPPRWMVA ENTFRPPWFH RNLMNEFMGL IQGAYDAKAG GFVPGGASLH 370 380 390 400 410 420 SCMSAHGPDA ESCDKAIAAD LKPHRIDQTM AFMFETSQVL RPSRAALETP ALQNDYDACW 430 ASLVSTFNPQ RR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Genes encoding phenylalanine catabolism in Pseudomonas aeruginosa." Beatson S.A., Whitchurch C.B., Mattick J.S. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 71-236. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE004091 Genomic DNA. Translation: AAG05397.1. AF109214 Genomic DNA. Translation: AAD23385.1.
PIR	F83394.
RefSeq	NP_250699.1. NC_002516.2.
3D structure databases
ProteinModelPortal	Q9X4G0.
SMR	Q9X4G0. Positions 142-200.
ModBase	Search...
Protein-protein interaction databases
STRING	208964.PA2009.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	877745.
KEGG	pae:PA2009.
PATRIC	19838425. VBIPseAer58763_2093.
Organism-specific databases
PseudoCAP	PA2009.
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEAE

Accession

Primary (citable) accession number: Q9X4G0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program