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Protein

Replicative DNA helicase

Gene

dnaB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity.UniRule annotation

Catalytic activityi

ATP + H2O = ADP + phosphate.UniRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: InterPro
  • identical protein binding Source: IntAct

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HelicaseUniRule annotationImported, Hydrolase

Keywords - Biological processi

DNA replicationUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-binding, DNA-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicative DNA helicaseUniRule annotation (EC:3.6.4.12UniRule annotation)
Gene namesi
Name:dnaBImported
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)Imported
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

PrimosomeUniRule annotation

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-6402993,EBI-6402993
dnaGQ9X4D05EBI-6402993,EBI-6403005

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-48437N.
IntActiQ9X4C9. 3 interactions.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R6AX-ray2.90A/B1-454[»]
2R6CX-ray4.00A/B/C/D/E/F1-454[»]
2R6DX-ray3.70A/B/C/D/E/F1-454[»]
2R6EX-ray5.02A/B1-454[»]
4ESVX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-454[»]
4M4WX-ray6.10A/B/C/D/E/F1-454[»]
ProteinModelPortaliQ9X4C9.
SMRiQ9X4C9. Positions 8-441.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X4C9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini179 – 445267SF4 helicaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the helicase family. DnaB subfamily.UniRule annotation
Contains 1 SF4 helicase domain.UniRule annotation

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9X4C9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSELFSERIP PQSIEAEQAV LGAVFLDPAA LVPASEILIP EDFYRAAHQK
60 70 80 90 100
IFHAMLRVAD RGEPVDLVTV TAELAASEQL EEIGGVSYLS ELADAVPTAA
110 120 130 140 150
NVEYYARIVE EKSVLRRLIR TATSIAQDGY TREDEIDVLL DEADRKIMEV
160 170 180 190 200
SQRKHSGAFK NIKDILVQTY DNIEMLHNRD GEITGIPTGF TELDRMTSGF
210 220 230 240 250
QRSDLIIVAA RPSVGKTAFA LNIAQNVATK TNENVAIFSL EMSAQQLVMR
260 270 280 290 300
MLCAEGNINA QNLRTGKLTP EDWGKLTMAM GSLSNAGIYI DDTPSIRVSD
310 320 330 340 350
IRAKCRRLKQ ESGLGMIVID YLQLIQGSGR SKENRQQEVS EISRSLKALA
360 370 380 390 400
RELEVPVIAL SQLSRSVEQR QDKRPMMSDI RESGSIEQDA DIVAFLYRDD
410 420 430 440 450
YYNKDSENKN IIEIIIAKQR NGPVGTVQLA FIKEYNKFVN LERRFDEAQI

PPGA
Length:454
Mass (Da):50,643
Last modified:November 1, 1999 - v1
Checksum:iD6115B868E11BAC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106032 Genomic DNA. Translation: AAD20314.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF106032 Genomic DNA. Translation: AAD20314.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2R6AX-ray2.90A/B1-454[»]
2R6CX-ray4.00A/B/C/D/E/F1-454[»]
2R6DX-ray3.70A/B/C/D/E/F1-454[»]
2R6EX-ray5.02A/B1-454[»]
4ESVX-ray3.20A/B/C/D/E/F/G/H/I/J/K/L1-454[»]
4M4WX-ray6.10A/B/C/D/E/F1-454[»]
ProteinModelPortaliQ9X4C9.
SMRiQ9X4C9. Positions 8-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48437N.
IntActiQ9X4C9. 3 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiQ9X4C9.

Family and domain databases

Gene3Di1.10.860.10. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR007692. DNA_helicase_DnaB.
IPR007694. DNA_helicase_DnaB-like_C.
IPR007693. DNA_helicase_DnaB-like_N.
IPR016136. DNA_helicase_N/primase_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00772. DnaB. 1 hit.
PF03796. DnaB_C. 1 hit.
[Graphical view]
SUPFAMiSSF48024. SSF48024. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00665. DnaB. 1 hit.
PROSITEiPS51199. SF4_HELICASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The Bacillus stearothermophilus replicative helicase: cloning, overexpression and activity."
    Bird L.E., Wigley D.B.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: NCA1503Imported.
  2. "Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase."
    Bailey S., Eliason W.K., Steitz T.A.
    Science 318:459-463(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.90 ANGSTROMS).
  3. "The hexameric helicase DnaB adopts a nonplanar conformation during translocation."
    Itsathitphaisarn O., Wing R.A., Eliason W.K., Wang J., Steitz T.A.
    Cell 151:267-277(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.20 ANGSTROMS).
  4. "Structure of a helicase-helicase loader complex reveals insights into the mechanism of bacterial primosome assembly."
    Liu B., Eliason W.K., Steitz T.A.
    Nat. Commun. 4:2495-2495(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (6.10 ANGSTROMS).

Entry informationi

Entry nameiQ9X4C9_GEOSE
AccessioniPrimary (citable) accession number: Q9X4C9
Entry historyi
Integrated into UniProtKB/TrEMBL: November 1, 1999
Last sequence update: November 1, 1999
Last modified: May 11, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureCombined sources

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.