Reviewed, UniProtKB/Swiss-Prot Q9X449 (ACKA2_RHIME)

Names and origin

Protein names

Recommended name:
    Acetate kinase
    EC=2.7.2.1
Alternative name(s):
    Acetokinase

Gene names

Name:

ackA

Organism

Rhizobium meliloti (Sinorhizobium meliloti)

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	393 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP MF_00020 ATP + propanoate = ADP + propanoyl phosphate. HAMAP MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP MF_00020
Subcellular location	Cytoplasm By similarity HAMAP MF_00020.
Sequence similarities	Belongs to the acetokinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	organic acid metabolic process Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 393	393	Acetate kinase HAMAP MF_00020		PRO_0000107604
Experimental info
	Sequence conflict	209 – 212	4	SGAS → AEFR in AAD42996. Ref.2
	Sequence conflict	227 – 238	12	LHRPS…CRWDT → GFTALDGLPMGTR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9X449-1 [UniParc]. Last modified November 1, 1999. Version 1. Checksum: 48FD185524CD6D4C	FASTA	393	42,135
	10 20 30 40 50 60 MDALLVVNAG SSSLKFQVFG IVGMDLTRQI RGKVDGIGTR PRLQATAADG TQLIDQTYDA 70 80 90 100 110 120 KAVRDLPAAI TEARRWLLTL EGFELQAVGH RVVHGGPDYT RPVLIDATVL DHLAGYQDLA 130 140 150 160 170 180 PLHQPNNLAP IRLAMEINPD VPQVACFDTA FHRGHAKHTD CYALPRSFYD EGVRRYGFHG 190 200 210 220 230 240 LSYEYIAERL REVASRAAKG RVVVAHLGSG ASMCGLRDGR SIESTMLHRP STGCRWDTPG 250 260 270 280 290 300 QLDPGVVLYL ILQKGMKAQA VSDLLYHDAG LKGLSGLSND MRDLLASDDP HAALSVAHFV 310 320 330 340 350 360 YRCVLNGGML AAALGGIDAF VFTAGVGENS PPIRARIVEG LAWLGAELDP AANEAGAALI 370 380 390 STATSRVAVH VLPTDEELMI ARHTLALISA PNA

References

[1]	"Genes coding for phosphotransacetylase and acetate kinase in Sinorhizobium meliloti are in an operon that is inducible by phosphate stress and controlled by phoB." Summers M.L., Denton M.C., McDermott T.R. J. Bacteriol. 181:2217-2224(1999) [PubMed: 10094701] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 104A14.
[2]	"Expression and regulation of phosphate stress inducible genes in Sinorhizobium meliloti." Summers M.L., Elkins J.G., Elliott B.A., McDermott T.R. Mol. Plant Microbe Interact. 11:1094-1101(1998) [PubMed: 9805396] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 207-314. Strain: 104A14.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF095903 Genomic DNA. Translation: AAD24358.1. AF074452 Genomic DNA. Translation: AAD42996.1.
3D structure databases
SMR	Q9X449. Positions 4-389.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.1. 142.
Family and domain databases
HAMAP	MF_00020. Acetate_kinase. [Tree]
InterPro	IPR000890. Acetate/butyrate_kinase. IPR004372. AckA. [Graphical view]
PANTHER	PTHR21060. Acetate_kin. 1 hit. PTHR21060:SF11. AckA. 1 hit.
Pfam	PF00871. Acetate_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000722. Acetate_prop_kin. 1 hit.
PRINTS	PR00471. ACETATEKNASE.
TIGRFAMs	TIGR00016. ackA. 1 hit.
PROSITE	PS01075. ACETATE_KINASE_1. 1 hit. PS01076. ACETATE_KINASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACKA2_RHIME

Accession

Primary (citable) accession number: Q9X449
Secondary accession number(s): Q9XDG2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1999
Last modified:	February 9, 2010
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)