Reviewed, UniProtKB/Swiss-Prot Q9X3X7 (TOP1_ZYMMO)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Omega-protein
    Relaxing enzyme
    Untwisting enzyme
    Swivelase

Gene names

Name:	topA
Ordered Locus Names:	ZMO1193

Organism

Zymomonas mobilis [Complete proteome] [HAMAP]

Taxonomic identifier

542 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas

Protein attributes

Sequence length	1217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Subunit structure	Monomer By similarity.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family.

Ontologies

Keywords
Domain	Zinc-finger
Ligand	ATP-binding DNA-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1217	1217	DNA topoisomerase 1		PRO_0000145175
Regions
	Zinc finger	597 – 624	28	C4-type
	Compositional bias	1114 – 1212	99	Lys-rich
Sites
	Active site	298	1	For DNA cleavage activity By similarity
Experimental info
	Sequence conflict	176 – 177	2	VA → AR in AAD21553. Ref.1
	Sequence conflict	450	1	V → F in AAD21553. Ref.1
	Sequence conflict	486	1	V → F in AAD21553. Ref.1
	Sequence conflict	1022	1	S → F in AAD21553. Ref.1
	Sequence conflict	1179	1	K → I in AAD21553. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9X3X7-1 [UniParc]. Last modified February 15, 2005. Version 2. Checksum: C14C004EA0BC7755	FASTA	1,217	131,276
	10 20 30 40 50 60 MVRSIMKLVV VESPAKAKTI EKYLGSGYQV LASYGHIRDL PSKDGSVNPD NGFSMVWQNY 70 80 90 100 110 120 PDKAHRLKAI EDAVKESDSL ILATDPDREG EAISWHILEL LKTKKLLPDD VERVTFNAIT 130 140 150 160 170 180 KAAVTDAMAH PRQLDNDLIN AYLARRALDY LVGFTLSPIL WRKLPGAKSA GRVQSVALRL 190 200 210 220 230 240 VVDREQEIEG FKPQEYWSVE ADMEADSIGF TSRLIQWRGQ KLEKLSIADK TQAEAAQSDV 250 260 270 280 290 300 ESGHFTVSNV ETKPVSRNPQ PPFTTSTLQQ EAARKLGFAA SHTMRLAQSL YEEGLITYMR 310 320 330 340 350 360 TDGVQMDESA IAAARKTITQ RYDGGYVPDK PRQYQVKAKN AQEAHEAIRP TDFSRDKAAS 370 380 390 400 410 420 GDHARLYELI WKRALASQMA SAKLERTTVD LTDGTGQNVL RVTGQVVLFS GFLTLYEESA 430 440 450 460 470 480 DDNANDRDGK EGRARLPLLR AGDAPLKKEV RADQHFTQPP PRYSEASLVK KMEDLGIGRP 490 500 510 520 530 540 STYASVIQVL KDRAYVTLER NRFIPSEAGR LLTAFLERFF ERYVSYDFTA GLEDSLDEIS 550 560 570 580 590 600 GGRADWQKVL DAFWHDFKPK TAEVMEQQPS AITAELDKFL EPWLFPDKGD GHDPRECPSC 610 620 630 640 650 660 HTGRLALKGG RFGAFIACSN YPECKYTRGF GQGEDGVDDN EPVLLGYMPE NSDDDGYMAF 670 680 690 700 710 720 SDQGDILPAN TASASETGVT DGGIAANAAF SGKGNSASHT DRDDLPFDPD EPASSTGNVA 730 740 750 760 770 780 SSQSRMTGDE TASSGNSRDS SAHGVSTTAG IDAESQAGIS NQALAGKNNA GRTAVSDNKG 790 800 810 820 830 840 NNSSSTIAAA RKGGSTDDNA TVSDPDGDIG SGASSSGQDA DNRLLSHRNG DIDSRAIPAD 850 860 870 880 890 900 HKDSSSDQNA SHALSPDRNS DDASVSNSDK KIDSKAVSTG HDVGNAITSD NSPSDNVAHL 910 920 930 940 950 960 ASTPSSATSS VKVALETENN DTAASKADEQ AKEEEESRKA RAVTRRTGRF GPYIQLGEGK 970 980 990 1000 1010 1020 NAKRVSVPRD VNPREVDFSL ASRLLALPRE IGLHPESGKM IIAGTGRYGP YLNCDGKYAR 1030 1040 1050 1060 1070 1080 LSSTEELLDI DIDRAVVKLA EAAQNKGRTG ATLSREPLKV FGDSPVTEKP VQLMNGRYGP 1090 1100 1110 1120 1130 1140 YVTDGETNAS LPKDTTPESL TFEEALALLE ARAKMPKKKK TKKAAAKKPA AKKTTTKKAA 1150 1160 1170 1180 1190 1200 PKKATTKTAT PKSATTDNAT SEDGDATPAK SPAKKAVAKK TTAKKPASKS ATKKAPSSKT 1210 TAAKKTSKAT PKDEVAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Lee H.J., Kang H.S. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed: 15592456] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF088896 Genomic DNA. Translation: AAD21553.1. AE008692 Genomic DNA. Translation: AAV89817.1.
RefSeq	YP_162928.1.
3D structure databases
HSSP	HSSP built from PDB template 1ECL based on UniProtKB P06612.
ModBase	Search...
Genome annotation databases
GeneID	3189286.
GenomeReviews	Gene locus ZMO1193 in contig AE008692_GR.
KEGG	zmo:ZMO1193.
NMPDR	fig|264203.3.peg.386.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9X3X7.
Enzyme and pathway databases
BioCyc	ZMOB264203:ZMO1193-MON.
BRENDA	5.99.1.2. 1658.
Family and domain databases
InterPro	IPR003601. Topo_IA_2. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013826. Topo_IA_cen_sub3. IPR000380. Topo_IA_core. IPR003602. Topo_IA_DNA_bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac. IPR006154. Toprim_dom_subgr. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:1.10.290.10. Topo_IA_cen_sub3. 1 hit.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 1 hit. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
TIGRFAMs	TIGR01051. topA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TOP1_ZYMMO

Accession

Primary (citable) accession number: Q9X3X7
Secondary accession number(s): Q5NN93

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	February 15, 2005
Last modified:	July 28, 2009
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)